Effects of the genotoxic carcinogen chromium(VI) on basal and hormone-inducible phosphoenolpyruvate carboxykinase gene expression in vivo: Correlation with glucocorticoid-and developmentally regulated expression

1994 ◽  
Vol 10 (4) ◽  
pp. 189-198 ◽  
Author(s):  
Jennifer McCaffrey ◽  
Chad M. Wolf ◽  
Joshua W. Hamilton
Keyword(s):  
Gene Expression ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Genotoxic Carcinogen ◽  
Developmentally Regulated ◽  
Chromium Vi ◽  
Regulated Expression ◽  
Phosphoenolpyruvate Carboxykinase Gene

scholarly journals Developmentally regulated interactions of liver nuclear factors with the rat phosphoenolpyruvate carboxykinase promoter.

1990 ◽  
Vol 10 (5) ◽  
pp. 2418-2422 ◽  
Author(s):  
M Trus ◽  
N Benvenisty ◽  
H Cohen ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fetal Liver ◽  
Sequential Pattern ◽  
Late Development ◽  
Developmentally Regulated ◽  
Nuclear Factors

A sequential pattern of interactions of trans-acting factors in rat liver with the phosphoenolpyruvate carboxykinase promoter during late development was observed. A liver-enriched factor, possibly AF1, interacted with the promoter in fetal liver, whereas a factor with the characteristics of C/EBP bound the promoter after birth with the onset of the gene expression.

Hormonal Regulation of Phosphoenolpyruvate Carboxykinase Gene Expression

1985 ◽  
pp. 347-368 ◽  
Author(s):  
David S. Loose ◽  
Anthony Wynshaw-Boris ◽  
Herman M. Meisner ◽  
Yaacov Hod ◽  
Richard W. Hanson
Keyword(s):  
Gene Expression ◽  
Hormonal Regulation ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Phosphoenolpyruvate Carboxykinase Gene

Developmentally regulated interactions of liver nuclear factors with the rat phosphoenolpyruvate carboxykinase promoter

1990 ◽  
Vol 10 (5) ◽  
pp. 2418-2422
Author(s):  
M Trus ◽  
N Benvenisty ◽  
H Cohen ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fetal Liver ◽  
Sequential Pattern ◽  
Late Development ◽  
Developmentally Regulated ◽  
Nuclear Factors

A sequential pattern of interactions of trans-acting factors in rat liver with the phosphoenolpyruvate carboxykinase promoter during late development was observed. A liver-enriched factor, possibly AF1, interacted with the promoter in fetal liver, whereas a factor with the characteristics of C/EBP bound the promoter after birth with the onset of the gene expression.

scholarly journals Tumorigenicity of simian virus 40-hepatocyte cell lines: effect of in vitro and in vivo passage on expression of liver-specific genes and oncogenes.

1988 ◽  
Vol 8 (10) ◽  
pp. 4492-4501 ◽  
Author(s):  
C D Woodworth ◽  
J W Kreider ◽  
L Mengel ◽  
T Miller ◽  
Y L Meng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Tumor Cell ◽  
Cell Lines ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Tumor Cell Lines ◽  
Glutathione S Transferase

Five simian virus 40 (SV40)-hepatocyte cell lines were examined for tumorigenicity and the effect of in vitro passage on the expression of four liver-specific genes (albumin, transferrin, alpha 1-antitrypsin, and phosphoenolpyruvate carboxykinase), two oncogenes (c-Ha-ras and c-raf), and two genes associated with hepatocarcinogenesis (alpha-fetoprotein and placental-type glutathione-S-transferase). At low passage (12 to 22), all five cell lines expressed the four liver-specific genes at levels similar to those in the liver and were not tumorigenic or were weakly tumorigenic. At high passage (33 to 61), the cell lines formed carcinomas, and four out of five cell lines produced primary tumors that metastasized. At least two cell lines produced well-differentiated hepatocellular carcinomas that expressed liver-specific RNAs. Levels of expression of liver-specific genes changed with time in culture. Some of the changes in liver-specific gene expression in the tumor tissue (such as for the phosphoenolpyruvate carboxykinase gene) paralleled those that occurred with in vitro passage, while other changes (such as for the albumin gene) did not parallel those that occurred with in vitro passage. Correlations between enhanced expression of c-Ha-ras and tumorigenic potential and between the process of SV40 immortalization and induced expression of c-raf and glutathione-S-transferase-P were observed. Induction of alpha-fetoprotein was detected with in vitro and in vivo passage only in the CWSV14 cell line and was paralleled by diminished albumin expression. In conclusion, we developed a model system with five SV40-hepatocyte cell lines, tumors induced by them, and tumor cell lines to examine changes in gene expression that accompany the progression from a normal cell to a hepatocellular carcinoma. Because the SV40-hepatocyte cell lines and tumor cell lines remain highly differentiated and vary in the magnitude of expression of specific genes, they can be used to study the molecular mechanisms regulating gene expression, in particular those regulating specific genes associated with differentiation.

The genotoxic carcinogen chromium(VI) alters the metal-inducible expression but not the basal expression of the metailothionein gene in vivo

1994 ◽  
Vol 15 (5) ◽  
pp. 1089-1092 ◽  
Author(s):  
Joy A. Alcedo ◽  
Manoj Misra ◽  
Joshua W. Hamilton ◽  
Karen E. Wetterhahn
Keyword(s):  
Inducible Expression ◽  
Genotoxic Carcinogen ◽  
Chromium Vi ◽  
Basal Expression
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