Effects of emulsifiers on an in vitro model of intestinal epithelial tight junctions and the transport of food allergens

2021 ◽  
pp. 2100576
Author(s):  
Sefat E Khuda ◽  
Ann V. Nguyen ◽  
Girdhari M. Sharma ◽  
Mohammad S. Alam ◽  
Kannan V. Balan ◽  
...  
Keyword(s):  
Tight Junctions ◽  
In Vitro Model ◽  
Intestinal Epithelial ◽  
Food Allergens ◽  
Epithelial Tight Junctions ◽  
Vitro Model

Villus contraction aids repair of intestinal epithelium after injury

1989 ◽  
Vol 257 (2) ◽  
pp. G274-G283 ◽  
Author(s):  
R. Moore ◽  
S. Carlson ◽  
J. L. Madara
Keyword(s):  
In Vitro Model ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Villus Height ◽  
Epithelial Barrier Function ◽  
Epithelial Repair ◽  
Vitro Model ◽  
Energy Dependent ◽  
Cytoplasmic Processes

A highly reproducible in vitro model of intestinal epithelial injury in guinea pig ileum was used to study the structural and functional events that accompany rapid epithelial repair. This model is characterized by denudation of the villus tip followed by rapid restitution of the epithelial barrier. Using standard electrophysiological and quantitative morphometric techniques, we found that immediately after injury the number of cells lost exceeded the number of empty cell positions on the denuded basement membrane by 100%. Concurrently, cytoplasmic processes of subepithelial myofibroblasts contained condensations of microfilaments that were not apparent in controls. Additionally, villus height was diminished immediately after injury, and progressively decreased during the restitution period. In tissues that were depleted of ATP using the uncoupler dinitrophenol and in tissues functionally denervated by tetrodotoxin, villus shortening after injury was significantly reduced. Denervation also retarded functionally and structurally defined reestablishment of epithelial barrier function. These data suggest that intestinal epithelial repair is aided by energy-dependent, neurally mediated villus contraction. We speculate that the subepithelial network of myofibroblasts is responsible for this process, which effectively minimizes the denuded surface area to be reepithelialized.

Effect of amyloid curli fibrils and curli CsgA monomers from Escherichia coli on in vitro model of intestinal epithelial barrier stimulated with cytokines

2019 ◽  
Vol 309 (5) ◽  
pp. 274-282
Author(s):  
Beata Sobieszczańska ◽  
Barbara Pawłowska ◽  
Anna Duda-Madej ◽  
Krzysztof Pawlik ◽  
Jerzy Wiśniewski ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
In Vitro Model ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Vitro Model
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