Intraoviductal introduction of plasmid DNA and subsequent electroporation for efficient in vivo gene transfer to murine oviductal epithelium

2005 ◽  
Vol 71 (3) ◽  
pp. 321-330 ◽  
Author(s):  
Masahiro Sato



2012 ◽  
Vol 9 (7) ◽  
pp. 1962-1970 ◽  
Author(s):  
Shintaro Fumoto ◽  
Sayuri Nakajima ◽  
Toyoharu Mine ◽  
Naoki Yoshikawa ◽  
Takashi Kitahara ◽  
...  


1996 ◽  
Vol 7 (5) ◽  
pp. 589-593 ◽  
Author(s):  
Tetsuo Takehara ◽  
Norio Hayashi ◽  
Masato Yamamoto ◽  
Yasuhide Miyamoto ◽  
Hideyuki Fusamoto ◽  
...  


2004 ◽  
Vol 51 (3) ◽  
pp. 693-702 ◽  
Author(s):  
Aleksander Sochanik ◽  
Tomasz Cichoń ◽  
Monika Makselon ◽  
Małgorzata Strózyk ◽  
Ryszard Smolarczyk ◽  
...  

This report describes gene transfer in vitro as well as in vivo using cetylated low-molecular mass (600 Da) polyethylenimine (28% of amine groups substituted with cetyl moieties), termed CT-PEI. This compound is hydrophobic and has to be incorporated into liposomes in order to be suitable for gene transfer studies. Serum-induced plasmid DNA degradation assay demonstrated that CT-PEI-containing liposomal carriers could protect complexed DNA (probably via condensation). In vitro luciferase gene expression achieved using medium supplemented with 10% serum was comparable to that achieved in serum-reduced medium and was highest for CT-PEI/cholesterol liposomes, followed by CT-PEI/dioleoylphosphatidylcholine liposomes and PEI 600 Da (uncetylated) carrier. In vivo systemic transfer into mice was most efficient when liposome formulations contained CT-PEI and cholesterol. Higher luciferase expression was then observed in lungs than in liver. liposomes containing cetylated polyethylenimine and cholesterol are a suitable vehicle for investigating systemic plasmid DNA transfer into lungs.





2007 ◽  
Vol 177 (4S) ◽  
pp. 617-618
Author(s):  
Hiraki Kubota ◽  
Kevin Coward ◽  
Olivia Hibbitt ◽  
Nilendran Prathalingam ◽  
William Holt ◽  
...  






2006 ◽  
Vol 32 (5) ◽  
pp. 449-453 ◽  
Author(s):  
Pierre Collinet ◽  
Rodolphe Vereecque ◽  
Frédéric Sabban ◽  
Denis Vinatier ◽  
Eric Leblanc ◽  
...  


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