Cloning and characterization of a new gene from the PAT protein family, in a marsupial, the stripe-faced dunnart (Sminthopsis macroura )

2010 ◽  
Vol 77 (4) ◽  
pp. 373-383 ◽  
Author(s):  
Phil Chi Khang Au ◽  
Lynne Selwood ◽  
Mary Familari
Keyword(s):  
Protein Family ◽  
New Gene ◽  
Sminthopsis Macroura ◽  
Pat Protein

scholarly journals Biochemical characterization of specific Alanine Decarboxylase (AlaDC) and its ancestral enzyme Serine Decarboxylase (SDC) in tea plants (Camellia sinensis)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Peixian Bai ◽  
Liyuan Wang ◽  
Kang Wei ◽  
Li Ruan ◽  
Liyun Wu ◽  
...  
Keyword(s):  
Gene Duplication ◽  
Camellia Sinensis ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Protein Sequences ◽  
Biochemical Properties ◽  
High Similarity ◽  
New Gene ◽  
Tea Plants

Abstract Background Alanine decarboxylase (AlaDC), specifically present in tea plants, is crucial for theanine biosynthesis. Serine decarboxylase (SDC), found in many plants, is a protein most closely related to AlaDC. To investigate whether the new gene AlaDC originate from gene SDC and to determine the biochemical properties of the two proteins from Camellia sinensis, the sequences of CsAlaDC and CsSDC were analyzed and the two proteins were over-expressed, purified, and characterized. Results The results showed that exon-intron structures of AlaDC and SDC were quite similar and the protein sequences, encoded by the two genes, shared a high similarity of 85.1%, revealing that new gene AlaDC originated from SDC by gene duplication. CsAlaDC and CsSDC catalyzed the decarboxylation of alanine and serine, respectively. CsAlaDC and CsSDC exhibited the optimal activities at 45 °C (pH 8.0) and 40 °C (pH 7.0), respectively. CsAlaDC was stable under 30 °C (pH 7.0) and CsSDC was stable under 40 °C (pH 6.0–8.0). The activities of the two enzymes were greatly enhanced by the presence of pyridoxal-5′-phosphate. The specific activity of CsSDC (30,488 IU/mg) was 8.8-fold higher than that of CsAlaDC (3467 IU/mg). Conclusions Comparing to CsAlaDC, its ancestral enzyme CsSDC exhibited a higher specific activity and a better thermal and pH stability, indicating that CsSDC acquired the optimized function after a longer evolutionary period. The biochemical properties of CsAlaDC might offer reference for theanine industrial production.

Characterization of Spi-B, a transcription factor related to the putative oncoprotein Spi-1/PU.1

1992 ◽  
Vol 12 (10) ◽  
pp. 4297-4304 ◽  
Author(s):  
D Ray ◽  
R Bosselut ◽  
J Ghysdael ◽  
M G Mattei ◽  
A Tavitian ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
New Gene ◽  
Carboxy Terminal Region ◽  
Carboxy Terminal ◽  
Hematopoietic Cell Lines

We have cloned a human cDNA from a new gene, spi-B, on the basis of its homology with the DNA-binding domain of the Spi-1/PU.1 putative oncogene product. spi-B codes for a protein of 262 amino acids presenting 43% overall identity with Spi-1. Its highly basic carboxy-terminal region exhibits 34% sequence identity with the DNA-binding domain of the Ets-1 protein. We showed that the Spi-B protein is able to bind the purine-rich sequence (PU box) recognized by Spi-1/PU.1 and to activate transcription of a reporter plasmid containing PU boxes. Chromosome in situ hybridization allowed us to map spi-B to the 19q13.3-19q13.4 region of the human genome. spi-B, like spi-1, was found to be expressed in various murine and human hematopoietic cell lines except T lymphoid cell lines.

scholarly journals Characterization of the pixB gene in Xenorhabdus nematophila and discovery of a new gene family

Microbiology ◽  
2018 ◽  
Vol 164 (4) ◽  
pp. 495-508 ◽  
Author(s):  
John Lucas ◽  
Mary Goetsch ◽  
Matt Fischer ◽  
Steven Forst
Keyword(s):  
Gene Family ◽  
Xenorhabdus Nematophila ◽  
New Gene

scholarly journals Meiosis in Polyploids and Implications for Genetic Mapping: A Review

Genes ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1517
Author(s):  
Nina Reis Soares ◽  
Marcelo Mollinari ◽  
Gleicy K. Oliveira ◽  
Guilherme S. Pereira ◽  
Maria Lucia Carneiro Vieira
Keyword(s):  
Oilseed Rape ◽  
Genetic Maps ◽  
Crop Species ◽  
Map Construction ◽  
Complex Process ◽  
New Gene ◽  
Specific Proteins ◽  
Bivalent Formation ◽  
Essential Knowledge

Plant cytogenetic studies have provided essential knowledge on chromosome behavior during meiosis, contributing to our understanding of this complex process. In this review, we describe in detail the meiotic process in auto- and allopolyploids from the onset of prophase I through pairing, recombination, and bivalent formation, highlighting recent findings on the genetic control and mode of action of specific proteins that lead to diploid-like meiosis behavior in polyploid species. During the meiosis of newly formed polyploids, related chromosomes (homologous in autopolyploids; homologous and homoeologous in allopolyploids) can combine in complex structures called multivalents. These structures occur when multiple chromosomes simultaneously pair, synapse, and recombine. We discuss the effectiveness of crossover frequency in preventing multivalent formation and favoring regular meiosis. Homoeologous recombination in particular can generate new gene (locus) combinations and phenotypes, but it may destabilize the karyotype and lead to aberrant meiotic behavior, reducing fertility. In crop species, understanding the factors that control pairing and recombination has the potential to provide plant breeders with resources to make fuller use of available chromosome variations in number and structure. We focused on wheat and oilseed rape, since there is an abundance of elucidating studies on this subject, including the molecular characterization of the Ph1 (wheat) and PrBn (oilseed rape) loci, which are known to play a crucial role in regulating meiosis. Finally, we exploited the consequences of chromosome pairing and recombination for genetic map construction in polyploids, highlighting two case studies of complex genomes: (i) modern sugarcane, which has a man-made genome harboring two subgenomes with some recombinant chromosomes; and (ii) hexaploid sweet potato, a naturally occurring polyploid. The recent inclusion of allelic dosage information has improved linkage estimation in polyploids, allowing multilocus genetic maps to be constructed.

scholarly journals Characterization of COMMD protein–protein interactions in NF-κB signalling

2006 ◽  
Vol 398 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Prim de Bie ◽  
Bart van de Sluis ◽  
Ezra Burstein ◽  
Karen J. Duran ◽  
Ruud Berger ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Copper Metabolism ◽  
Nuclear Factor Κb ◽  
Inhibitory Effect ◽  
Protein Family ◽  
Amino Acid Residues ◽  
Protein Protein Interactions ◽  
Necrosis Factor

COMMD [copper metabolism gene MURR1 (mouse U2af1-rs1 region 1) domain] proteins constitute a recently identified family of NF-κB (nuclear factor κB)-inhibiting proteins, characterized by the presence of the COMM domain. In the present paper, we report detailed investigation of the role of this protein family, and specifically the role of the COMM domain, in NF-κB signalling through characterization of protein–protein interactions involving COMMD proteins. The small ubiquitously expressed COMMD6 consists primarily of the COMM domain. Therefore COMMD1 and COMMD6 were analysed further as prototype members of the COMMD protein family. Using specific antisera, interaction between endogenous COMMD1 and COMMD6 is described. This interaction was verified by independent techniques, appeared to be direct and could be detected throughout the whole cell, including the nucleus. Both proteins inhibit TNF (tumour necrosis factor)-induced NF-κB activation in a non-synergistic manner. Mutation of the amino acid residues Trp24 and Pro41 in the COMM domain of COMMD6 completely abolished the inhibitory effect of COMMD6 on TNF-induced NF-κB activation, but this was not accompanied by loss of interaction with COMMD1, COMMD6 or the NF-κB subunit RelA. In contrast with COMMD1, COMMD6 does not bind to IκBα (inhibitory κBα), indicating that both proteins inhibit NF-κB in an overlapping, but not completely similar, manner. Taken together, these data support the significance of COMMD protein–protein interactions and provide new mechanistic insight into the function of this protein family in NF-κB signalling.

scholarly journals Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium.

1997 ◽  
Vol 179 (13) ◽  
pp. 4399-4402 ◽  
Author(s):  
E Webb ◽  
K Claas ◽  
D M Downs
Keyword(s):  
Salmonella Typhimurium ◽  
New Gene

FUNCTIONAL CHARACTERIZATION OF AN LCCL–LECTIN DOMAIN CONTAINING PROTEIN FAMILY IN PLASMODIUM BERGHEI

2004 ◽  
Vol 90 (5) ◽  
pp. 1062-1071 ◽  
Author(s):  
Holly E. Trueman ◽  
J. Dale Raine ◽  
Laurence Florens ◽  
Johannes T. Dessens ◽  
Jacqui Mendoza ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Functional Characterization ◽  
Protein Family ◽  
Lectin Domain

Extensive Characterization of the Human Salivary Basic Proline-Rich Protein Family by Top-Down Mass Spectrometry

2018 ◽  
Vol 17 (9) ◽  
pp. 3292-3307 ◽  
Author(s):  
Alessandra Padiglia ◽  
Roberto Orrù ◽  
Mozhgan Boroumand ◽  
Alessandra Olianas ◽  
Barbara Manconi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Family ◽  
Top Down ◽  
Proline Rich Protein ◽  
Top Down Mass Spectrometry

Identification and characterization of rDJL, a novel member of the DnaJ protein family, in rat testis

FEBS Letters ◽  
2005 ◽  
Vol 579 (25) ◽  
pp. 5734-5740 ◽  
Author(s):  
Chunbo Yang ◽  
Shiying Miao ◽  
Shudong Zong ◽  
Samuel S. Koide ◽  
Linfang Wang
Keyword(s):  
Protein Family ◽  
Rat Testis ◽  
Dnaj Protein ◽  
Identification And Characterization
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