Successful Nonsurgical Management of a Traumatic Dens Fracture in a Cat with Clinical and Radiographic Resolution

ABSTRACT An adult domestic shorthair presented with obtundation, vestibular ataxia, head tilt, and visible evidence of facial injury following motor vehicle trauma. Plain radiographs and computed tomography imaging revealed a complete minimally displaced transverse fracture of the caudal aspect of the dens of the C2 vertebra and multiple minimally displaced cranial fractures. The dens fracture was managed with 8 wk of strict rest, followed by 4 wk of supervised activity at home. No external immobilization was performed. Neurological examinations at 8 days, 10 wk, and 9 mo following initial presentation were normal. Repeat radiographic and computed tomography examinations at 10 wk and 9 mo following the traumatic event demonstrated progressive and eventual complete osseous union of the fractured dens. To the authors’ knowledge, this is the first report of successful nonsurgical management of a traumatic dens fracture in an adult cat with documented radiographic and clinical resolution. This report suggests that nonsurgical management can be considered in such cats and that complete resolution with osseous union is feasible.

Case Report: Primary Diffuse Leptomeningeal Oligodendrogliomatosis in a Young Adult Cat

A 2-year-old cat was presented with progressive ataxia. Despite treatment the animal died. Pathomorphological examination revealed a widespread leptomeningeal mass at all levels of the central nervous system accentuated on the cervical spinal cord and the medulla oblongata without presence of a primary intraaxial tumor. The neoplasm was mainly composed of round, uninucleate cells with hyperchromatic nuclei, which were immunopositive for OLIG2, doublecortin, MAP2, synaptophysin, and vimentin, indicating components of both oligodendroglial and neuronal differentiation. Ki-67 immunohistochemistry indicated a high proliferation activity of the neoplasm. Few GFAP positive and Iba-1 positive cells were interpreted as reactive astrocytes and macrophages or microglia, respectively. The tumor was immunonegative for CD3, CD20, PAX5, MUM1, pan-cytokeratin, S100, NSE, p75NTR, NeuN and periaxin. These findings led to the diagnosis of primary diffuse leptomeningeal oligodendrogliomatosis. This is the first reported case of this entity in a young cat, which should be considered as a differential diagnosis for diffuse subarachnoidal round cell infiltrates.

118 The Use of Meta-analysis in Companion Animal Research

In a world of the “Three Rs” (replace, reduce and refine), combined with more research published via open access research journals, there is increasing interest in the statistical analysis of existing literature. Meta-analysis – the combination of multiple studies, can be used to get better oversight into a specific question of interest. Additionally, it can be used to identify gaps in knowledge. For example, while there are a number of publications investigating energy requirements in adult cat and dog, few studies assess older animals. Similarly, in the dog, there is a lack of literature around dogs at the extremes of body size (i.e. giant and toy breeds). Herein, we describe several published examples that have been used to determine energy requirements of cats and dogs, and more recently, the impacts of diet on the microbiome of the cat and dog. This includes the use of Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) guidelines, research findings and general findings related to research design and quality.

A-177 Classification Statistics of the Clinical Assessment of Attention Deficit – Adult (CAT-A) Negative Impression Scale for ADHD Evaluations in an Active Duty Sample

Objective To evaluate the classification statistics of the Clinical Assessment of Attention Deficit – Adult (CAT-A) Negative Impression (NI) scale, relative to the MMPI-2-RF in an active duty military sample. We hypothesized that MMPI-2-RF RBS would have the largest AUC. Method Retrospective review of 108 active duty service members consecutively referred to neuropsychology for clinical ADHD evaluations in a Midwest Army Health Center. Cases with missing data (10) and failures of MMPI-2-RF response consistency scales (3) were removed from analysis, resulting in final sample of 95. Average age was 32.62 (SD = 8.87) and average education was 14.66 (SD = 2.66). MMPI-2-RF symptom validity scales (F-r, Fp-r, Fs, FBS-r, and RBS) were employed as criterion measures at all standard cut-offs to evaluate the CAT-A NI scale. Results ROC analyses for the MMPI-2-RF over-reporting symptom validity scales ranged from 0.599 to 0.808, with the MMPI-2-RF RBS scale >79 demonstrating the lowest AUC (0.599; p 52. With specificity held at or above 0.90, sensitivities ranged from 22.86 to 60.00, with positive and negative predictive values ranging from 11.1 to 58.8 and 66.7 to 98.8, respectively. Conclusions The CAT-A NI scale demonstrated reasonable AUC values for nearly all MMPI-2-RF over-reporting scales. Psychologically-based over-reporting scales were superior to cognitively-based over-reporting scales in terms of classification statistics. The optimal cut in the current data (>52) is slightly higher (though roughly commensurate) to the cut score for atypical responses in the CAT-A manual (>45).

A Validation of Supervised Deep Learning for Gait Analysis in the Cat

Gait analysis in cats and other animals is generally performed with custom-made or commercially developed software to track reflective markers placed on bony landmarks. This often involves costly motion tracking systems. However, deep learning, and in particular DeepLabCutTM (DLC), allows motion tracking without requiring placing reflective markers or an expensive system. The purpose of this study was to validate the accuracy of DLC for gait analysis in the adult cat by comparing results obtained with DLC and a custom-made software (Expresso) that has been used in several cat studies. Four intact adult cats performed tied-belt (both belts at same speed) and split-belt (belts operating at different speeds) locomotion at different speeds and left-right speed differences on a split-belt treadmill. We calculated several kinematic variables, such as step/stride lengths and joint angles from the estimates made by the two software and assessed the agreement between the two measurements using intraclass correlation coefficient or Lin’s concordance correlation coefficient as well as Pearson’s correlation coefficients. The results showed that DLC is at least as precise as Expresso with good to excellent agreement for all variables. Indeed, all 12 variables showed an agreement above 0.75, considered good, while nine showed an agreement above 0.9, considered excellent. Therefore, deep learning, specifically DLC, is valid for measuring kinematic variables during locomotion in cats, without requiring reflective markers and using a relatively low-cost system.

Diversified physiological sensory input connectivity questions the existence of distinct classes of spinal interneurons in the adult cat in vivo

The spinal cord is engaged in all forms of motor performance but its functions are far from understood. Because network connectivity defines function, we explored the connectivity for muscular, tendon and tactile sensory inputs among a wide population of spinal interneurons in the lower cervical segments. Using low noise intracellular whole cell recordings in the decerebrated, non-anesthetized cat in vivo, we could define mono-, di-, trisynaptic inputs as well as the weights of each input. Whereas each neuron had a highly specific input, and each indirect input could moreover be explained by inputs in other recorded neurons, we unexpectedly also found the input connectivity of the spinal interneuron population to form a continuum. Our data hence contrasts with the currently widespread notion of distinct classes of interneurons. We argue that this more diversified physiological connectivity, which likely requires a major component of circuitry learning, implies a more flexible functionality.

Oocyte Meiotic Competence in the Domestic Cat Model: Novel Roles for Nuclear Proteins BRD2 and NPM1

To participate in fertilization and embryo development, oocytes stored within the mammalian female ovary must resume meiosis as they are arrested in meiotic prophase I. This ability to resume meiosis, known as meiotic competence, requires the tight regulation of cellular metabolism and chromatin configuration. Previously, we identified nuclear proteins associated with the transition from the pre-antral to the antral follicular stage, the time at which oocytes gain meiotic competence. In this study, the objective was to specifically investigate three candidate nuclear factors: bromodomain containing protein 2 (BRD2), nucleophosmin 1 (NPM1), and asparaginase-like 1 (ASRGL1). Although these three factors have been implicated with folliculogenesis or reproductive pathologies, their requirement during oocyte maturation is unproven in any system. Experiments were conducted using different stages of oocytes isolated from adult cat ovaries. The presence of candidate factors in developing oocytes was confirmed by immunostaining. While BRD2 and ASRGL1 protein increased between pre-antral and the antral stages, changes in NPM1 protein levels between stages were not observed. Using protein inhibition experiments, we found that most BRD2 or NPM1-inhibited oocytes were incapable of participating in fertilization or embryo development. Further exploration revealed that inhibition of BRD2 and NPM-1 in cumulus-oocyte-complexes prevented oocytes from maturing to the metaphase II stage. Rather, they remained at the germinal vesicle stage or arrested shortly after meiotic resumption. We therefore have identified novel factors playing critical roles in domestic cat oocyte meiotic competence. The identification of these factors will contribute to improvement of domestic cat assisted reproduction and could serve as biomarkers of meiotically competent oocytes in other species.

Prevalence of gastro-intestinal parasitic infections of cats and efficacy of antiparasitics against these infections in Mymensingh sadar, Bangladesh

Background: Gastro-intestinal parasitic infections in cats is a major concern for public health as they have zoonotic importance. The present research was conducted to determine the prevalence of gastro-intestinal parasitic infection in cats in different areas of Mymensingh Sadar between December, 2018 to May, 2019. Methods: The fecal samples were examined by simple sedimentation and stoll’s ova counting method for detection of eggs/cysts/oocysts of parasites. The efficacy of antiparasitics against the parasitic infections in cats was evaluated. Results: The overall prevalence of gastrointestinal parasites was 62.9% (39/62) and the mixed parasitic infection was 20.9% (13/62). The prevalence of Toxocara cati and Ancylostoma tubaeforme infections were 17.7% and 6.5%, respectively. The prevalence of Taenia pisiformis infection was 3.22%. However, the prevalence of Isospora felis, Toxoplasma gondii and Balantidium coli infections were 4.8%, 3.2% and 6.5%. The prevalence of infection was significantly (P<0.008) higher in kitten than that in adult cat. The efficacy of albendazole, fenbendazole against single helminth infection was 100%. However, the efficacy of the drug was reduced against mixed helminth infection. The efficacy of metronidazole, pyrimithamine plus sulfadoxine was 100% against single protozoa infection but the efficacy of the drug was reduced against mixed protozoal infection. Conclusions: Kittens should be dewormed with effective anthelmintics as they are more vulnerable to parasitic diseases. The T. cati, A. tubaeforme, T. gondii prevalent in cats are zoonotic and owners awareness need to be increased to prevent human infections.

Botanicals Reduce Circulating Concentrations of Cholesterol and Triglycerides and Work Synergistically With Arachidonic Acid to Reduce Inflammatory Cytokines in Cats

Forty Eight cats were used to measure the effects of feeding a traditional adult cat food supplemented with either arachidonic acid (ARA), a botanical mix (botanicals) or both on circulating biochemical parameters and inflammatory cytokines. The cats were healthy adults (mean age, 3.0; range, 1.3–6.4 years). The adult cats were fed one of four foods (n = 12 per group) for 84 days (dietary changes reported as fed): a traditional adult cat food (control, 0.05% ARA no added botanicals), or control food supplemented with arachidonic acid from chicken liver (0.13% ARA when supplemented), control food supplemented with botanicals (green tea 0.5%, fenugreek 0.05%, and tulsi 0.003%), and control plus ARA (0.13% as fed) with botanicals (green tea 0.5%, fenugreek 0.05%, and tulsi 0.003%). Response variables were compared between treatments: initially, and at 84 days (end of study). The measurements were standard complete blood counts and chemistries as well as circulating cytokines. Botanical inclusion reduced (P &lt; 0.05) circulating cholesterol and triglycerides while arachidonic acid increased (P &lt; 0.05) their concentrations. The pro-inflammatory cytokines MCP-1, TNFα, SDF-1, Flt3L, IL-8, IL-12p40, IL-13, and IL-18 were all reduced (P &lt; 0.05) in cats after consuming the ARA + botanicals food for 84 days with little change after consuming the other foods. Therefore, this combination of ARA and botanicals may be of value in reducing inflammation.