Uptake and disposal of bsa-coated latex particles by the rat mesangium: Reaction with subsequently administered heterologous antiserum

1985 ◽  
Vol 147 (3) ◽  
pp. 189-198 ◽  
Author(s):  
Nigel P. Goode ◽  
Alex M. Davison ◽  
Gerald Gowland ◽  
Samuel R. Aparicio ◽  
Michael Shires
Keyword(s):  
Latex Particles ◽  
Heterologous Antiserum

A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 314-315
Author(s):  
Earl R. Walter ◽  
Glen H. Bryant
Keyword(s):  
Sample Preparation ◽  
High Vacuum ◽  
Vacuum System ◽  
Latex Particles ◽  
New Methods ◽  
Diffusion Pump ◽  
Film Forming ◽  
Routine Basis ◽  
Distribution Studies ◽  
Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

Increased Protein Synthesis by Human Platelets during Phagocytosis of Latex Particles in Vitro

1976 ◽  
Vol 35 (02) ◽  
pp. 350-357 ◽  
Author(s):  
Hana Bessler ◽  
Galila Agam ◽  
Meir Djaldetti
Keyword(s):  
Protein Synthesis ◽  
Fold Increase ◽  
Human Platelets ◽  
Polystyrene Latex ◽  
Latex Particles ◽  
The Third ◽  
Platelet Protein ◽  
Dose Dependent ◽  
Phagocytotic Activity

SummaryA three-fold increase of protein synthesis by human platelets during in vitro phagocytosis of polystyrene latex particles was detected. During the first two hours of incubation, the percentage of phagocytizing platelets and the number of latex particles per platelet increased; by the end of the third hour, the first parameter remained stable, while the number of latex particles per cell had decreased.Vincristine (20 μg/ml of cell suspension) inhibited platelet protein synthesis. This effect was both time- and dose-dependent. The drug also caused a decrease in the number of phagocytizing cells, as well as in their phagocytotic activity.

Physiological Effects of Nonimmune Platelet Associated Immunoglobulin G

1981 ◽  
Vol 45 (01) ◽  
pp. 027-033 ◽  
Author(s):  
K Sugiura ◽  
M Steiner ◽  
M Baldini
Keyword(s):  
Shape Change ◽  
Fluorescein Isothiocyanate ◽  
Recovery Phase ◽  
Human Platelets ◽  
Serotonin Release ◽  
Platelet Volume ◽  
Igg Antibody ◽  
Heterologous Antiserum ◽  
Igg Binding ◽  
Series Of Experiments

SummaryThe function of nonimmune IgG associated with platelets is unknown. In a series of experiments we have investigated this problem, relating amount of platelet-associated IgG (PAIgG) to platelet volume, serotonin release, adherence of platelets to monocytes and platelet senescence. Most of these studies were performed with human platelets. Platelets freed of preexisting PAIgG by incubation at 22° C were incubated with IgG in a series of concentrations ranging from 0.4 — 27.0 X10-6 M. The IgG preparations used were demonstrably free of aggregated forms of the protein. The amount of PAIgG bound to platelets was determined by the use of fluorescein isothiocyanate-conjugated anti-IgG antibody (F-anti-IgG antibody) which was quantified in a fluorospectrophotometer. Newly bound IgG was assayed similarly by the use of F-IgG. A dose-dependent increase in platelet volume was associated with the binding of nonimmune IgG by platelets. The process which leveled off at an IgG concentration of 1.2 —1.5 X10-5 M was almost fully reversible and was not due to platelet shape change or aggregation. Release of serotonin from IgG-treated platelets was relatively small but to the extent that it occurred was positively related to the IgG concentration to which platelets were exposed. Adherence to autologous monocytes studied quantitatively by the use of formaldehyde-fixed cells was also positively related to the amount of IgG on the platelets. Normal or IgG-defident serum had a potent inhibitory (noncompetitive) action on the binding of F-IgG and F-anti-human IgG antibody to human platelets. Cohorts of platelets prepared in rabbits during the recovery phase of immunological thrombocytopenia induced by injection of heterologous antiserum, showed an age-dependent increase of PAIgG and of IgG binding. These results suggest that PAIgG plays a role in the clearance of senescent platelets.

Recent Applications of Nonradiative Energy Transfer to Polymer Studies

1993 ◽  
Vol 58 (10) ◽  
pp. 2266-2271 ◽  
Author(s):  
Herbert Morawetz
Keyword(s):  
Energy Transfer ◽  
Polymer Chain ◽  
Cluster Formation ◽  
Nonradiative Energy Transfer ◽  
Molecular Cluster ◽  
Fluorescent Labels ◽  
Latex Particles ◽  
Polymer Latex

Recent studies of polymers in solution and in bulk by energy transfer between two fluorescent labels are reviewed. Such studies are concerned with the equilibrium and dynamics of polymer chain expansion, molecular cluster formation in solution, the miscibility of polymers in bulk, and the interdiffusion of polymer latex particles.

Synthesis and Characterization of Polypyrrole-Coated Sulfur-Rich Latex Particles:  New Synthetic Mimics for Sulfur-Based Micrometeorites

2006 ◽  
Vol 18 (11) ◽  
pp. 2758-2765 ◽  
Author(s):  
Syuji Fujii ◽  
Steven P. Armes ◽  
Richard Jeans ◽  
Robin Devonshire ◽  
Samantha Warren ◽  
...  
Keyword(s):  
Synthesis And Characterization ◽  
Latex Particles

scholarly journals Cellulose Nanocrystals Mimicking Micron-Sized Fibers to Assess the Deposition of Latex Particles on Cotton

2021 ◽  
Author(s):  
Evdokia K. Oikonomou ◽  
Konstantin Golemanov ◽  
Pierre-Emmanuel Dufils ◽  
James Wilson ◽  
Ritu Ahuja ◽  
...  
Keyword(s):  
Cellulose Nanocrystals ◽  
Latex Particles

scholarly journals Microsuspension Polymerization of Styrene Using Cellulose Nanocrystals as Pickering Emulsifiers: On the Evolution of Latex Particles

Langmuir ◽  
2019 ◽  
Vol 36 (3) ◽  
pp. 796-809 ◽  
Author(s):  
Joe Glasing ◽  
Philip G. Jessop ◽  
Pascale Champagne ◽  
Wadood Y. Hamad ◽  
Michael F. Cunningham
Keyword(s):  
Cellulose Nanocrystals ◽  
Latex Particles ◽  
Microsuspension Polymerization
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