In vitro study of HIF-1 activation and VEGF release by bFGF in the T47D breast cancer cell line under normoxic conditions: involvement of PI-3K/Akt and MEK1/ERK pathways

2005 ◽  
Vol 205 (4) ◽  
pp. 530-536 ◽  
Author(s):  
Yong-hong Shi ◽  
Yu-xiang Wang ◽  
Lynne Bingle ◽  
Li-hua Gong ◽  
Wan-jie Heng ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
In Vitro Study ◽  
T47d Breast Cancer Cell ◽  
Vegf Release

scholarly journals Improving the Efficacy of Cisplatin using Niosome Nanoparticles Against Human Breast Cancer Cell Line BT-20 : An In Vitro Study

2017 ◽  
Vol 2 (2) ◽  
pp. 27-29 ◽  
Author(s):  
Leila Kanaani ◽  
Maral Mazloumi Tabrizi ◽  
Azim Akbarzadeh Khiyavi ◽  
Iraj Javadi
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
In Vitro Study ◽  
Initial Response ◽  
Lethal Effect

Objective: Today, cancer is one the most important challenges in modern medicine. Meanwhile, breast cancer is one of the most common causes of mortality among cancers. The initial response to the treatment and then becoming resistant to the cisplatin is one of basal challenges of treatment of breast cancer. Recently, using nanotechnology including drug nanocarrier named niosome can decrease adverse effects and increase the efficiency of treatment. Material and Methods: The aim of this research was to investigate using niosome nanoparticles containing cisplatin and investigation of their lethal effect on breast cancer cell line. Results: We found that using niosome nanoparticles can provide a suitable formulation of cisplatin drug. Conclusion: The efficiency of nanoniosome cisplatin was more than free drug, decreasing the administered dose and therefore the damage to other tissues.

scholarly journals The effect of ethanolic and petroleum ether fractions of bawang dayak (Eleutherine palmifolia) bulb extract on expression of p53 mutant in breast cancer cell line T47D

2010 ◽  
Vol 8 (1) ◽  
pp. 17-26
Author(s):  
IVAN HENDRA SUDARMAWAN ◽  
DJOKO DLIDIR ◽  
AMBAR MUDIGDO ◽  
DYAH RATNA BUDIANI
Keyword(s):  
Breast Cancer ◽  
Petroleum Ether ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
T47d Breast Cancer Cell ◽  
Significant Difference

Sudarmawan IH, Dlilir D, Mudigdo A, Budiani DR. 2010. The effect of ethanolic and petroleum ether fractions of bawang dayak (Eleutherine palmifolia) bulb extract on expression of p53 mutant in breast cancer cell line T47D. Biofarmasi 8: 17-26. Breast cancer was still to be the most popular disease. The second highest morbidity and mortality stages after cervix cancer which need to be involved in alternative therapy. Bawang dayak (Eleutherine palmifolia (L.) Merr ) had been used as anti-cancer in empiric therapy by urban society. Therefore, it was needed to determine the influence of etanolic and petroleum eter fractions of bawang dayak extract on the p53 mutant expression in T47D breast cancer cell in vitro. Epithelial cell in ductal mammae breast cancer T47D which had a malignancy and p53 mutant, with ER/PR positive status. As a comparation, it was used MCF7 cell specimen (negative control). They were cultured on 60 wells for T47D and MCF7 in RPMI 1640 media. Each well was filled with 2x105 cells/200 µl media. Thirty wells with a size of 1.5 cm/T47D cell diameter, cultured in etanolic and petroleum eter fractions with under concentration of LC50. The number of sample was determined by using “Rule of Thumb”. The result of LC50 on T47D breast cancer cell were etanolic fraction 125 Î¼g/mL, while petroleum eter fraction 31.25 μg/mL. The result of expression percentage of p53 mutant given by the extract of etanolic fraction of bawang dayak were: 0 μg/mL = 36.11%, 15.625 μg/mL = 28.32%, 31.25 μg/mL = 27.46%, 62.5 μg/mL = 19.67%, and 125 Î¼g/mL = 11.02%. Other result of bawang dayak extract percentage given in petroleum eter fraction were: 0 μg/mL = 26.16%, 3.90625 Î¼g/mL = 25.29%, 7.8125 μg/mL = 22.70%, 15.625 μg/mL = 22.27%, and 31.25 μg/mL =15.78%. The treatment of bawang dayak extract in ethanolic and petroleum eter fractions were able to inhibit the expression of p53 mutant in vitro. The result of this research showed no significant difference in the inhibition expression of p53 mutant in breast cancer cell T47D.

scholarly journals The Effect of Nano-Transferosomal Hydroxyurea on 4T1 animal Breast Cancer Cell Line: An in Vitro Study

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Maral Mazloumi Tabrizi ◽  
Pouria Mohammadi ◽  
Afrooz Ahmadi ◽  
Mohammadreza Malekpour ◽  
Nahid Kabiry ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals Formulation of Piperine–Chitosan-Coated Liposomes: Characterization and In Vitro Cytotoxic Evaluation

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3281
Author(s):  
Syed Sarim Imam ◽  
Sultan Alshehri ◽  
Mohammad A. Altamimi ◽  
Afzal Hussain ◽  
Wajhul Qamar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Therapeutic Efficacy ◽  
Cancer Cell Line ◽  
Permeation Study ◽  
Study Results

The present research work is designed to prepare and evaluate piperine liposomes and piperine–chitosan-coated liposomes for oral delivery. Piperine (PPN) is a water-insoluble bioactive compound used for different diseases. The prepared formulations were evaluated for physicochemical study, mucoadhesive study, permeation study and in vitro cytotoxic study using the MCF7 breast cancer cell line. Piperine-loaded liposomes (PLF) were prepared by the thin-film evaporation method. The selected liposomes were coated with chitosan (PLFC) by electrostatic deposition to enhance the mucoadhesive property and in vitro therapeutic efficacy. Based on the findings of the study, the prepared PPN liposomes (PLF3) and chitosan coated PPN liposomes (PLF3C1) showed a nanometric size range of 165.7 ± 7.4 to 243.4 ± 7.5, a narrow polydispersity index (>0.3) and zeta potential (−7.1 to 29.8 mV). The average encapsulation efficiency was found to be between 60 and 80% for all prepared formulations. The drug release and permeation study profile showed biphasic release behavior and enhanced PPN permeation. The in vitro antioxidant study results showed a comparable antioxidant activity with pure PPN. The anticancer study depicted that the cell viability assay of tested PLF3C2 has significantly (p < 0.001)) reduced the IC50 when compared with pure PPN. The study revealed that oral chitosan-coated liposomes are a promising delivery system for the PPN and can increase the therapeutic efficacy against the breast cancer cell line.

scholarly journals Identifikasi Ekspresi Gen Myc pada Subkultur MCF-7 Breast Cancer Cell Line dengan Sel Punca

2018 ◽  
Vol 7 (3) ◽  
pp. 424
Author(s):  
Yuastika Puspita Sari ◽  
Hirowati Ali ◽  
Aswiyanti Asri
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Luminal A ◽  
Post Hoc ◽  
Mcf 7

Kanker payudara yang paling banyak ditemukan adalah subtipe luminal A dengan karakteristik Estrogen Reseptor+. Subjek penelitian akan diwakili oleh cell line MCF-7 dan Umbilical Cord Blood Mesenchymal Stem Cell (UCBMSC). Over expression Myc pada kanker payudara mengakibatkan sel kanker menjadi lebih invasif dan terjadi resistensi terhadap terapi hormonal. Tujuan penelitian ini adalah mengidentifikasi ekspresi gen Myc pada cell line MCF-7 sebelum dan sesudah pemberian sel punca. Penelitian ini menggunakan desain experimental secara in vitro. Sampel menggunakan MCF-7 dan sel punca yang dibagi menjadi 4 kelompok, yaitu K1 (kelompok kontrol MCF-7), K2 (kelompok kontrol sel punca), P1 (perlakuan subkultur MCF-7 dengan sel punca inkubasi 24 jam), dan P2 (inkubasi 48 jam). Kemudian, dilakukan isolasi RNA, sintesis cDNA, dan ekspresi Myc diperiksa menggunakan PCR dan elektroforesis. Analisa data yang digunakan adalan One Way ANOVA dan post-hoc LSD. Hasil analisis bivariat didapatkan p<0,05. Dari uji post-hoc LSD tidak ditemukan perbedaan yang bermakna antara K1 dengan P1, K1 dengan P2, dan K2 dengan P2. Namun, tetap ditemukan perbedaan yang bermakna antara K2 dengan P1 dan P1 dengan P2. Simpulan penelitian ini adalah tidak terdapat perbedaan bermakna ekspresi gen Myc pada subkultur antara MCF-7 breast cancer cell line dengan pemberian sel punca mesenkimal.

scholarly journals In vitro antineoplastic activity in triple-negative breast cancer cell line and in vivo

2014 ◽  
Vol 8 (Suppl 4) ◽  
pp. P22
Author(s):  
Klesia Madeira ◽  
Murilo Cerri ◽  
Renata Daltoé ◽  
Alice Herlinger ◽  
João Filho ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Triple Negative Breast Cancer ◽  
Breast Cancer Cell Line ◽  
Triple Negative ◽  
Cancer Cell Line
Sign in / Sign up

Export Citation Format

Share Document