Myeloma cells regulate miRNA transfer from fibroblast‐derived exosomes by expression of lncRNAs

A thickening of ER membranes in murine myeloma cells was attributed by de Harven to the assembly of intracisternal virus particles. We observed similar thickening of GER membranes in Friend leukemia cells (FLC) apparently associated with Friend leukemia virus (FLV) assembly. We reinvestigated the problem of GER involvement in FLV assembly using high pressure cryofixed FLC.FLC (745A clone growing in suspension and FF clone growing in monolayer) were immersed in Hexadecene (Fluka, Switzerland) and rapidly frozen in Balzers HPM 010 freezing machine working at 2200 bar. All cells were freeze substituted at -90°C in 2% OsO4 in absolute acetone. Serial sections cut to avoid misinterpretations due to the geometry of sections, were collected on carbon coated 100 mesh grids.

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Faculty Opinions recommendation of Proteasome inhibitors disrupt the unfolded protein response in myeloma cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1015247.196201 ◽

2003 ◽

Author(s):

David Ron

Keyword(s):

Unfolded Protein Response ◽

Proteasome Inhibitors ◽

Myeloma Cells ◽

Unfolded Protein ◽

The Unfolded Protein Response ◽

Protein Response

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Faculty Opinions recommendation of A small molecule inhibitor of ubiquitin-specific protease-7 induces apoptosis in multiple myeloma cells and overcomes bortezomib resistance.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.717957380.793461863 ◽

2012 ◽

Author(s):

Pengbo Zhou ◽

Jeffrey Hannah

Keyword(s):

Multiple Myeloma ◽

Small Molecule ◽

Small Molecule Inhibitor ◽

Myeloma Cells ◽

Molecule Inhibitor ◽

Specific Protease ◽

Ubiquitin Specific Protease

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Faculty Opinions recommendation of Lenalidomide causes selective degradation of IKZF1 and IKZF3 in multiple myeloma cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.718194536.793522194 ◽

2016 ◽

Author(s):

Parameswaran Hari ◽

Binod Dhakal

Keyword(s):

Multiple Myeloma ◽

Myeloma Cells ◽

Selective Degradation

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Betulinic Acid Enhances the Sensitivity of Multiple Myeloma Cells to Bortezomib by the Inactivation of the AKT/mTOR Pathway

Current Topics in Nutraceutical Research ◽

10.37290/ctnr2641-452x.18:241-246 ◽

2020 ◽

Vol 18 (3) ◽

pp. 241-246

Author(s):

Yu Dan ◽

Wan Sheng ◽

Hu Lili

Keyword(s):

Multiple Myeloma ◽

Betulinic Acid ◽

Myeloma Cell ◽

Mtor Pathway ◽

Prolonged Treatment ◽

Myeloma Cells ◽

Multiple Myeloma Cell ◽

Increased Sensitivity ◽

Colony Number ◽

Rpmi 8226

This study aimed to investigate the mechanism of betulinic acid on multiple myeloma cell resistance to bortezomib. To this end, the bortezomib-resistant RPMI-8226-R cells were generated by prolonged treatment of RPMI-8226 cells with increasing concentrations of bortezomib. Based on the measurements of cell viability and colony number, RPMI-8226-R cells exhibited enhanced resistance to bortezomib than RPMI-8226 cells. Treatment with betulinic acid resulted in increased sensitivity of RPMI-8226-R to bortezomib. When RPMI-8226-R cells were co-treated with bortezomib and betulinic acid, there was an increase in apoptosis rate, cleaved caspase-3, cleaved caspase-9 expression and the decrease in p-AKT/AKT and p-mTOR/mTOR levels. These results suggest that betulinic acid enhances the sensitivity of RPMI-8226-R cells to bortezomib by inhibiting the activation of the AKT/mTOR pathway in bortezomib-resistant multiple myeloma cells.

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