Two-dimensional electrophoresis for the isolation of integral membrane proteins and mass spectrometric identification

PROTEOMICS ◽  
2004 ◽  
Vol 4 (9) ◽  
pp. 2567-2571 ◽  
Author(s):  
Isam Rais ◽  
Michael Karas ◽  
Hermann Schägger
Keyword(s):  
Membrane Proteins ◽  
Mass Spectrometric ◽  
Integral Membrane Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification ◽  
Dimensional Electrophoresis

Characterization of microvillar membrane proteins of dog small intestine by two-dimensional electrophoresis

1995 ◽  
Vol 110 (3) ◽  
pp. 483-492 ◽  
Author(s):  
Philip W. Pemberton ◽  
Robert W. Lobley ◽  
Raymond Holmes ◽  
Susanne H. Sørensen ◽  
Kenneth W. Simpson ◽  
...  
Keyword(s):  
Small Intestine ◽  
Membrane Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Two-dimensional electrophoresis of membrane proteins

2007 ◽  
Vol 389 (4) ◽  
pp. 1033-1045 ◽  
Author(s):  
Ralf J. Braun ◽  
Norbert Kinkl ◽  
Monika Beer ◽  
Marius Ueffing
Keyword(s):  
Membrane Proteins ◽  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Enhancing the mass spectrometric identification of membrane proteins by combining chemical and enzymatic digestion methods

2015 ◽  
Vol 7 (17) ◽  
pp. 7220-7227 ◽  
Author(s):  
Johanna M. Smeekens ◽  
Weixuan Chen ◽  
Ronghu Wu
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Analysis ◽  
Enzymatic Digestion ◽  
Mass Spectrometric ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification ◽  
Digestion Methods

Combining chemical and enzymatic digestion methods proved to be extremely effective for mass-spectrometry (MS)-based membrane protein analysis.

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