Enhancing the mass spectrometric identification of membrane proteins by combining chemical and enzymatic digestion methods

2015 ◽  
Vol 7 (17) ◽  
pp. 7220-7227 ◽  
Author(s):  
Johanna M. Smeekens ◽  
Weixuan Chen ◽  
Ronghu Wu
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Protein Analysis ◽  
Enzymatic Digestion ◽  
Mass Spectrometric ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification ◽  
Digestion Methods

Combining chemical and enzymatic digestion methods proved to be extremely effective for mass-spectrometry (MS)-based membrane protein analysis.

Improved mass spectrometric identification of gel-separated hydrophobic membrane proteins after sodium dodecyl sulfate removal by ion-pair extraction

PROTEOMICS ◽  
2004 ◽  
Vol 4 (12) ◽  
pp. 3776-3782 ◽  
Author(s):  
Hans Zischka ◽  
Christian Johannes Gloeckner ◽  
Christian Klein ◽  
Stefanie Willmann ◽  
Magdalena Swiatek-de Lange ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Ion Pair ◽  
Mass Spectrometric ◽  
Hydrophobic Membrane ◽  
Sulfate Removal ◽  
Spectrometric Identification ◽  
Dodecyl Sulfate ◽  
Mass Spectrometric Identification

Stoffwechselprodukte von Mikroorganismen, 146 Massenspektrometrische Identifizierung von 3-Cyclohexenylglycin im Kulturfiltrat von Streptomyces tendae / Metabolic Products of Microorganisms, 146 Mass Spectrometric Identification of 3-Cyclohexenylglycine from Streptomyces tendae

1975 ◽  
Vol 30 (7-8) ◽  
pp. 626-628 ◽  
Author(s):  
W. A. König ◽  
H. Hagenmaier ◽  
U. Dähn
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Mass Spectrometric ◽  
Gas Chromatography Mass Spectrometry ◽  
Culture Filtrates ◽  
Chromatography Mass Spectrometry ◽  
Spectrometric Identification ◽  
Metabolic Products ◽  
Mass Spectrometric Identification ◽  
Streptomyces Tendae

From the culture filtrates of Streptomyces tendae (Tü 901) a ninhydrin positive substance was isolated, which was identified as 3-cyclohexenylglycine by gas chromatography-mass spectrometry of some volatile derivatives.

Mass spectrometry of ozonides

1969 ◽  
Vol 47 (6) ◽  
pp. 919-934 ◽  
Author(s):  
J. Castonguay ◽  
M. Bertrand ◽  
J. Carles ◽  
S. Fliszár ◽  
Y. Rousseau
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectra ◽  
Mass Spectrometric ◽  
Fragment Ions ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification ◽  
Fragmentation Patterns

The mass spectra of some ozonides are reported, and the modes of formation of the major fragment ions tentatively described. The use of 18O-labelled compounds has proven to be useful in establishing fragmentation patterns. It is concluded that these follow a characteristic path, which is common to all the ozonides studied and permits mass spectrometric identification.

Sign in / Sign up

Export Citation Format

Share Document