2-D reference map of Bacillus anthracis vaccine strain A16R proteins

PROTEOMICS ◽  
2005 ◽  
Vol 5 (17) ◽  
pp. 4488-4495 ◽  
Author(s):  
Junjun Wang ◽  
Tianyi Ying ◽  
Hengliang Wang ◽  
Zhaoxing Shi ◽  
Mingzhu Li ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Reference Map

scholarly journals Use of Canonical Single Nucleotide Polymorphism (CanSNPs) to characterizeBacillus anthracisoutbreak strains in Zambia between 1990 and 2014

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5270 ◽  
Author(s):  
Antonio Fasanella ◽  
Luigina Serrecchia ◽  
Alexandra Chiaverini ◽  
Giuliano Garofolo ◽  
Geoffrey M. Muuka ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Genetic Structure ◽  
Bacillus Anthracis ◽  
Molecular Characterization ◽  
Vaccine Strain ◽  
Southern Africa ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Ancestral Origin

Anthrax caused byBacillus anthracisis an old and neglected zoonosis that continues to raise concerns in Southern Africa. In this study, twenty (20) slides with suspected isolates ofB. anthracisfrom anthrax cases between 1990 and 2014 and two (2) from that of a vaccine strain were analysed using MLVA with 15 VNTRs and CanSNPs test. The results from the CanSNPs indicate that all anthrax outbreaks in Zambia between 1990 and 2014 were caused by the lineage A.Br.005/006 of the clade A. This indicates a common ancestral origin of theB. anthraciscirculating in the country. This data has described several environmental, wildlife, livestock and human cases that occurred in a 24 year period, from the major areas where anthrax is endemic. The molecular characterization of isolates from anthrax outbreaks in Zambia has revealed a genetic structure in agreement with previous studies from neighbouring countries. Further studies are needed to elucidate how to better manage anthrax outbreaks and define the risk maps of Zambia.

scholarly journals Bovine abortion by a vaccine strain of Bacillus anthracis

2020 ◽  
Vol 50 (12) ◽  
Author(s):  
Daniele Mariath Bassuino ◽  
Franciele Maboni Siqueira ◽  
Guilherme Konradt ◽  
Andréia Vielmo ◽  
Verônica Machado Rolim ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Chromosomal Marker ◽  
Commercial Vaccine ◽  
Gram Staining ◽  
Microbiological Examination ◽  
Vaccinal Strain ◽  
High Level ◽  
Bovine Abortion ◽  
Residual Virulence

ABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, various organs showed hemorrhage, fibrin exudation, necrosis associated with countless bacillary bacterial clumps and severe neutrophilic inflammatory infiltrate. In the microbiological examination, numerous rough, nonhemolytic, gray and dry colonies with irregular edges were isolated from liver, lung and abomasum content samples. Gram staining revealed square-ended Gram-positive rods arranged in chains. B. anthracis identification was confirmed by detection of the molecular chromosomal marker Ba813. The genomes from the isolated B. anthracis (named SPV842_15) and from the isolated vaccinal strain (Brazilian vaccinal strain), which was recovered from a commercial vaccine used in the pregnant cow, were sequenced. Genomic comparisons displayed a high level of nucleotide identity in the comparisons between B. anthracis SPV842_15 and the B. anthracis Brazilian vaccinal strain (98,2%). Furthermore, in both strains, only the plasmid pX01 sequence was detected. Although, vaccination against anthrax is characterized by an elevated protective profile and very low residual virulence, immunization with Sterne strains can cause abortion in cattle, presumably by the plasmid pX01 toxins in rare or special situations.

scholarly journals Genome Sequence of the Soviet/Russian Bacillus anthracis Vaccine Strain 55-VNIIVViM

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Jason Farlow ◽  
Adam Kotorashvili
Keyword(s):  
Population Structure ◽  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Genome Sequence ◽  
Anthrax Vaccine ◽  
Phylogenetic Placement ◽  
Global Population

Bacillus anthracis strain 55-VNIIVViM is a live-attenuated nonencapsulated Soviet/Russian veterinary anthrax vaccine strain. We report here the genome of 55-VNIIVViM and confirm its phylogenetic placement in the global population structure of B. anthracis .

scholarly journals Genome Sequence of the Attenuated Carbosap Vaccine Strain of Bacillus anthracis

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
R. Harrington ◽  
B. D. Ondov ◽  
D. Radune ◽  
M. B. Friss ◽  
J. Klubnik ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Genome Sequence

scholarly journals Genome Sequence of Bacillus anthracis STI, a Sterne-Like Georgian/Soviet Vaccine Strain

2014 ◽  
Vol 2 (5) ◽  
Author(s):  
R. T. Okinaka ◽  
J. Challacombe ◽  
K. Drees ◽  
D. N. Birdsell ◽  
N. Janke ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Genome Sequence

scholarly journals Morphological and immunological characterization of anthrax vaccine in cattle

2014 ◽  
Vol 11 (1) ◽  
pp. 43-49 ◽  
Author(s):  
M Dipti ◽  
MM Rashid ◽  
MJ Ferdoush ◽  
P Roy ◽  
MAHNA Khan ◽  
...  
Keyword(s):  
Antibody Response ◽  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Antibiotic Sensitivity ◽  
Igg Antibody ◽  
Anthrax Vaccine ◽  
Post Immunization ◽  
Antibiotic Sensitivity Test ◽  
Bacterium Bacillus ◽  
High Level

Anthrax is a zoonotic disease caused by the bacterium Bacillus anthracis that normally affects animals, especially ruminants (such as cattle, goats, sheep, and horses) and humans. This study was planned to characterize the morphology of anthrax vaccine bacteria by using Gram’s stain, polychrome methylene blue stain, culture on nutrient agar and nutrient broth media and to determine the immunological status of this vaccine by indirect ELISA and slide agglutination test in cattle. Antibiotic sensitivity test of vaccine strain of bacteria was also done. This study provided evidence that vaccine strain of Bacillus anthracis was gram-positive rod-shaped bacteria appeared as single to short-chained bacilli with blunted ends. Serum from anthrax vaccinated cattle agglutinated anthrax antigen on Day 30 (+++ within 5 min and ++ within 7 min at 1:100 dilution of test sera) and Day 90 (+++ within 5 min at 1:100 dilution of test sera) of post immunization. Immunization of cattle with anthrax vaccine generated high level of anti-anthrax IgG antibody response at Day 30 (0.789}0.014) of post immunization and reached its peak at Day 90 (0.991}0.047). This study also provided evidence that anthrax vaccine bacteria were sensitive to penicillin, streptomycin, amoxicillin and kenamycin. It may be recommended that the anthrax vaccine currently in use in Bangladesh is proved to be effective in term of morphology of Bacillus antharis and raising anti anthrax IgG antibody response in cattle with no side effect. DOI: http://dx.doi.org/10.3329/bjvm.v11i1.17732 Bangl. J. Vet. Med. (2013). 11 (1): 43-49

scholarly journals Identification of the Immunogenic Spore and Vegetative Proteins of Bacillus anthracis Vaccine Strain A16R

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e57959 ◽  
Author(s):  
Xiankai Liu ◽  
Dongshu Wang ◽  
Jingxiao Ren ◽  
Chao Tong ◽  
Erling Feng ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Vaccine Strain
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