scholarly journals Bovine abortion by a vaccine strain of Bacillus anthracis

2020 ◽  
Vol 50 (12) ◽  
Author(s):  
Daniele Mariath Bassuino ◽  
Franciele Maboni Siqueira ◽  
Guilherme Konradt ◽  
Andréia Vielmo ◽  
Verônica Machado Rolim ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Chromosomal Marker ◽  
Commercial Vaccine ◽  
Gram Staining ◽  
Microbiological Examination ◽  
Vaccinal Strain ◽  
High Level ◽  
Bovine Abortion ◽  
Residual Virulence

ABSTRACT: This paper reports the abortion of a male Aberdeen Angus bovine by a vaccine strain of Bacillus anthracis, describing the pathological and microbiological findings and the genome sequence. Necropsy findings included multifocal areas of hemorrhage in different organs. Histologically, various organs showed hemorrhage, fibrin exudation, necrosis associated with countless bacillary bacterial clumps and severe neutrophilic inflammatory infiltrate. In the microbiological examination, numerous rough, nonhemolytic, gray and dry colonies with irregular edges were isolated from liver, lung and abomasum content samples. Gram staining revealed square-ended Gram-positive rods arranged in chains. B. anthracis identification was confirmed by detection of the molecular chromosomal marker Ba813. The genomes from the isolated B. anthracis (named SPV842_15) and from the isolated vaccinal strain (Brazilian vaccinal strain), which was recovered from a commercial vaccine used in the pregnant cow, were sequenced. Genomic comparisons displayed a high level of nucleotide identity in the comparisons between B. anthracis SPV842_15 and the B. anthracis Brazilian vaccinal strain (98,2%). Furthermore, in both strains, only the plasmid pX01 sequence was detected. Although, vaccination against anthrax is characterized by an elevated protective profile and very low residual virulence, immunization with Sterne strains can cause abortion in cattle, presumably by the plasmid pX01 toxins in rare or special situations.

scholarly journals Morphological and immunological characterization of anthrax vaccine in cattle

2014 ◽  
Vol 11 (1) ◽  
pp. 43-49 ◽  
Author(s):  
M Dipti ◽  
MM Rashid ◽  
MJ Ferdoush ◽  
P Roy ◽  
MAHNA Khan ◽  
...  
Keyword(s):  
Antibody Response ◽  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Antibiotic Sensitivity ◽  
Igg Antibody ◽  
Anthrax Vaccine ◽  
Post Immunization ◽  
Antibiotic Sensitivity Test ◽  
Bacterium Bacillus ◽  
High Level

Anthrax is a zoonotic disease caused by the bacterium Bacillus anthracis that normally affects animals, especially ruminants (such as cattle, goats, sheep, and horses) and humans. This study was planned to characterize the morphology of anthrax vaccine bacteria by using Gram’s stain, polychrome methylene blue stain, culture on nutrient agar and nutrient broth media and to determine the immunological status of this vaccine by indirect ELISA and slide agglutination test in cattle. Antibiotic sensitivity test of vaccine strain of bacteria was also done. This study provided evidence that vaccine strain of Bacillus anthracis was gram-positive rod-shaped bacteria appeared as single to short-chained bacilli with blunted ends. Serum from anthrax vaccinated cattle agglutinated anthrax antigen on Day 30 (+++ within 5 min and ++ within 7 min at 1:100 dilution of test sera) and Day 90 (+++ within 5 min at 1:100 dilution of test sera) of post immunization. Immunization of cattle with anthrax vaccine generated high level of anti-anthrax IgG antibody response at Day 30 (0.789}0.014) of post immunization and reached its peak at Day 90 (0.991}0.047). This study also provided evidence that anthrax vaccine bacteria were sensitive to penicillin, streptomycin, amoxicillin and kenamycin. It may be recommended that the anthrax vaccine currently in use in Bangladesh is proved to be effective in term of morphology of Bacillus antharis and raising anti anthrax IgG antibody response in cattle with no side effect. DOI: http://dx.doi.org/10.3329/bjvm.v11i1.17732 Bangl. J. Vet. Med. (2013). 11 (1): 43-49

Molecular typing of Iranian field isolatesMycoplasma synoviaeand their differentiation from the live commercial vaccine strain MS-H usingvlhAgene

2014 ◽  
Vol 55 (2) ◽  
pp. 148-156 ◽  
Author(s):  
Mohammad Ali Bayatzadeh ◽  
Seyed Ali Pourbakhsh ◽  
Abass Ashtari ◽  
Ali Reza Abtin ◽  
Mohammad Abdoshah
Keyword(s):  
Vaccine Strain ◽  
Molecular Typing ◽  
Commercial Vaccine

2-D reference map of Bacillus anthracis vaccine strain A16R proteins

PROTEOMICS ◽  
2005 ◽  
Vol 5 (17) ◽  
pp. 4488-4495 ◽  
Author(s):  
Junjun Wang ◽  
Tianyi Ying ◽  
Hengliang Wang ◽  
Zhaoxing Shi ◽  
Mingzhu Li ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Vaccine Strain ◽  
Reference Map

scholarly journals ECOLOGICAL AND MICROBIOLOGICAL EXAMINATION OF TERRITORIES NON-WELFARE FOR ANTHRAX IN THE REPUBLIC OF BURYATIA

2016 ◽  
pp. 22-28
Author(s):  
Z. F. Dugarzhapova ◽  
N. B. Badmaev ◽  
V. E. Takaishvili ◽  
E. V. Kravets ◽  
B. Z. Tsydypov ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Legal Status ◽  
Stationary Points ◽  
Aim Analysis ◽  
Novel Approach ◽  
Microbiological Examination ◽  
Analysis Of Results ◽  
The Republic ◽  
Bone Remains

Aim. Analysis of results of ecological and microbiological examination of territories nonwelfare for anthrax in territories of Barguzinsky and Kurumkansky districts of the Republic of Buryatia for justification of prophylaxis measures. Materials and methods. Space photographs were used and area and landscape signs of cattle grave sites were established. 174 samples of soil and 6 samples of bone remains of agricultural animals were obtained and studied. Results. Examination of 15 objects in 12 non-welfare for anthrax stationary points of the Republic of Buryatia was carried out in August 2014. A novel approach to detection of abandoned cattle grave sites, where DNA of Bacillus anthracis had been detected in samples, was developed and proposed. Conclusion. Ecological properties of soils 2 districts of the Republic facilitate prolonged conservation of B.anthracis in the environment. Measures of sanitary clean-up of non-welfare territories, utilization of biological waste and decision on legal status of abandoned objects are recommended.

Isolation and Properties of a Deuterium-Tolerant Mutant of Pasteurella tularensis

1970 ◽  
Vol 1 (1) ◽  
pp. 56-60
Author(s):  
Jerry J. Tulis ◽  
Henry T. Eigelsbach
Keyword(s):  
Vaccine Strain ◽  
Parent Strain ◽  
Laboratory Animals ◽  
Live Vaccine Strain ◽  
Inhibitory Effects ◽  
Tolerant Mutant ◽  
Schu S4 ◽  
Immunogenic Properties ◽  
Highly Virulent ◽  
Residual Virulence

A spontaneously occurring stable deuterium-tolerant mutant was selected from highly virulent Pasteurella tularensis strain SCHU S4; the frequency of mutants in the cell populations was between 10 −3 and 10 −4 . Upon cultivation of parent strain SCHU S4 in media containing D 2 O in lieu of H 2 O, inhibitory effects became manifest at 18% D 2 O and increased significantly beyond 54% D 2 O; mutant strain SCHU DT was not inhibited by media containing as much as 98% D 2 O. When cultivated in media containing D 2 O, mutant SCHU DT, in comparison with parent strain SCHU S4, showed a significant reduction in dermal and respiratory virulence for laboratory animals. Its immunogenic properties were comparable to viable P. tularensis vaccine strain LVS. Data on the comparative growth of various P. tularensis strains on media containing D 2 O suggested that “Old World” strains might be more tolerant to isotopic substitution of deuterium for protium than North American strains. Because of the residual virulence of SCHU DT for experimental animals it is believed to have limited value as a potential live vaccine strain.

Bacillus anthracis as a cause of bovine abortion – a necropsy case requiring special biosafety measures

2018 ◽  
Vol 160 (9) ◽  
pp. 547-552 ◽  
Author(s):  
M Dettwiler ◽  
K Mehinagic ◽  
S Gobeli Brawand ◽  
A Thomann ◽  
S Feyer ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Bovine Abortion

scholarly journals Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain

2018 ◽  
Vol 49 (1) ◽  
pp. 18-19 ◽  
Author(s):  
Franciele Maboni Siqueira ◽  
Samuel Paulo Cibulski ◽  
Fabiana Quoos Mayer ◽  
David Driemeier ◽  
Saulo Petinatti Pavarini ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Genome Sequencing ◽  
Virulent Strain ◽  
Vaccinal Strain

scholarly journals Effects of Long-Term Storage on Plasmid Stability in Bacillus anthracis

2005 ◽  
Vol 71 (12) ◽  
pp. 7778-7780 ◽  
Author(s):  
Chung K. Marston ◽  
Alex R. Hoffmaster ◽  
Kathy E. Wilson ◽  
Sandra L. Bragg ◽  
Brian Plikaytis ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Plasmid Stability ◽  
Linear Trend ◽  
Chromosomal Marker ◽  
Virulence Plasmids ◽  
Long Term Storage ◽  
Response Network ◽  
Significant Difference ◽  
Term Storage

ABSTRACT The plasmid profiles of 619 cultures of Bacillus anthracis which had been isolated and stored between 1954 and 1989 were analyzed using the Laboratory Response Network real-time PCR assay targeting a chromosomal marker and both virulence plasmids (pXO1 and pXO2). The cultures were stored at ambient temperature on tryptic soy agar slants overlaid with mineral oil. When data were stratified by decade, there was a decreasing linear trend in the proportion of strains containing both plasmids with increased storage time (P < 0.001). There was no significant difference in the proportion of strains containing only pXO1 or strains containing only pXO2 (P = 0.25), but there was a statistical interdependence between the two plasmids (P = 0.004). Loss of viability of B. anthracis cultures stored on agar slants is also discussed.

Sign in / Sign up

Export Citation Format

Share Document