RNA interference of NADPH-cytochrome P450 reductase of the rice brown planthopper,Nilaparvata lugens,increases susceptibility to insecticides

2014 ◽  
Vol 71 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Su Liu ◽  
Qing-Mei Liang ◽  
Wen-Wu Zhou ◽  
Yan-Dong Jiang ◽  
Qing-Zi Zhu ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rna Interference ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Cytochrome P450 Reductase ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase

Downregulation of NADPH‐cytochrome P450 reductase via RNA interference increases the susceptibility of Acyrthosiphon pisum to desiccation and insecticides

2021 ◽  
Author(s):  
Jian‐Wen Qiao ◽  
Yong‐Liang Fan ◽  
Bing‐Jin Wu ◽  
Tian‐Tian Bai ◽  
Ying‐Hao Wang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rna Interference ◽  
Acyrthosiphon Pisum ◽  
Cytochrome P450 Reductase ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase

scholarly journals RNA Interference of NADPH-Cytochrome P450 Reductase Results in Reduced Insecticide Resistance in the Bed Bug, Cimex lectularius

PLoS ONE ◽  
2012 ◽  
Vol 7 (2) ◽  
pp. e31037 ◽  
Author(s):  
Fang Zhu ◽  
Sarah Sams ◽  
Tim Moural ◽  
Kenneth F. Haynes ◽  
Michael F. Potter ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rna Interference ◽  
Insecticide Resistance ◽  
Cimex Lectularius ◽  
Cytochrome P450 Reductase ◽  
Bed Bug ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase

scholarly journals RNA interference of NADPH-cytochrome P450 reductase increases susceptibilities to multiple acaricides in Tetranychus urticae

2020 ◽  
Vol 165 ◽  
pp. 104550 ◽  
Author(s):  
Adekunle W. Adesanya ◽  
Antonio Cardenas ◽  
Mark D. Lavine ◽  
Douglas B. Walsh ◽  
Laura C. Lavine ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rna Interference ◽  
Tetranychus Urticae ◽  
Cytochrome P450 Reductase ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase

scholarly journals RNA interference of NADPH-Cytochrome P450 reductase increases susceptibilities to multiple acaricides in Tetranychus urticae

2019 ◽  
Author(s):  
Adekunle W. Adesanya ◽  
Antonio Cardenas ◽  
Mark D. Lavine ◽  
Doug B. Walsh ◽  
Laura C. Lavine ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Rna Interference ◽  
Tetranychus Urticae ◽  
Cytochrome P450s ◽  
List Type ◽  
Cytochrome P450 Reductase ◽  
Acaricide Resistance ◽  
Nadph Cytochrome ◽  
P450 Reductase ◽  
Nadph Cytochrome P450 Reductase

AbstractThe two-spotted spider mite, Tetranychus urticae, is a polyphagous pest feeding on over 1,100 plant species, including numerous highly valued economic crops. The control of T. urticae largely depends on the use of acaricides, which leads to pervasive development of acaricide resistance. Cytochrome P450-mediated metabolic detoxification is one of the major mechanisms of acaricide resistance in T. urticae. NADPH-cytochrome P450 reductase (CPR) plays as a crucial co-factor protein that donates electron(s) to microsomal cytochrome P450s to complete their catalytic cycle. This study seeks to understand the involvement of CPR in acaricide resistance in urticae. The full-length cDNA sequence of T. urticae’s CPR (TuCPR) was cloned and characterized. TuCPR was ubiquitously transcribed in different life stages of T. urticae and the highest transcription was observed in the nymph and adult stages. TuCPR was constitutively over-expressed in six acaricide resistant populations compared to a susceptible one. TuCPR transcriptional expression was also induced by multiple acaricides in a time-dependent manner. Down-regulation of TuCPR via RNA interference (RNAi) in T. urticae led to reduced enzymatic activities of TuCPR and cytochrome P450s, as well as a significant reduction of resistance to multiple acaricides, abamectin, bifenthrin, and fenpyroximate. The outcome of this study highlights CPR as a potential novel target for eco-friendly control of T. urticae and other related plant-feeding pests.HighlightsPipernoyl butoxide significantly reduced abamectin, bifenthrin, and fenpyroximate resistance in T. urticae populationsT. urticae’s cytochrome P450 reductase (TuCPR) was cloned, sequenced and phylogenetically analyzedAbamectin, bifenthrin and fenpyroximate treatment induced TuCPR gene expressionSilencing of TuCPR in T. urticae caused a reduction in acaricide resistance

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