Novaluron prevents oogenesis and oviposition by inducing ultrastructural changes in ovarian tissue of young adult Lygus lineolaris

2020 ◽  
Vol 76 (12) ◽  
pp. 4057-4063
Author(s):  
Beverly Catchot ◽  
Chance JH Anderson ◽  
Jeffrey Gore ◽  
Ryan Jackson ◽  
Kuntol Rakshit ◽  
...  
Keyword(s):  
Young Adult ◽  
Ovarian Tissue ◽  
Ultrastructural Changes ◽  
Lygus Lineolaris

Ultrastructural changes in avian toxic heterophils

1988 ◽  
Vol 46 ◽  
pp. 360-361
Author(s):  
S. Latimer ◽  
W.L. Steffens ◽  
Mark Goodwin
Keyword(s):  
Young Adult ◽  
The Other ◽  
Ultrastructural Changes ◽  
White Leghorn ◽  
Sterile Saline ◽  
Commercial Grade ◽  
Avian Cell ◽  
Blood Neutrophils ◽  
The Right ◽  
Morphologic Changes

In mammals, morphologic changes in peripheral blood neutrophils are often reflections of bacterial infection and severe inflammation. These light microscopic changes as evidenced by Romanowsky staining may include appearance of Döhle bodies, toxic granules, vacuolation, and cytoplasmic basophilia. Cells exhibiting such changes are referred to as “toxic” neutrophils. Studies of ultrastructural alterations in toxic neutrophils are reported and well-documented in humans (1) and dogs (2). Detailed controlled investigations of leukocyte changes in response to inflammation in birds are lacking. It was the purpose of this study to document toxic changes induced by intramuscular turpentine injection in the equivalent avian cell, the heterophil.Young adult female White Leghorn chickens were obtained and divided into 2 groups, one receiving injection with 1 ml of filter-sterilized commercial grade turpentine in the right breast muscle, and the other (controls) receiving a similar volume of sterile saline.

SURVIVAL TIME OF OVARIAN HOMOGRAFTS IN TWO STRAINS OF RATS

1956 ◽  
Vol 13 (2) ◽  
pp. 201-NP ◽  
Author(s):  
A. S. PARKES
Keyword(s):  
Young Adult ◽  
Medical Research ◽  
Survival Time ◽  
Histological Examination ◽  
Functional Activity ◽  
Ovarian Tissue ◽  
Corpora Lutea ◽  
Adult Females ◽  
Latent Interval ◽  
Follicular Cysts

SUMMARY 1. Autografts and intra- and inter-strain homografts of ovarian tissue were made among rats of the two strains, albino and hooded, maintained at the National Institute for Medical Research. 2. Young adult females were ovariectomized and ovarian tissue transplanted subcutaneously. Surviving animals were killed 12–13 months after operation and the grafts, where present, removed for histological examination. 3. The occurrence of vaginal cornification was taken to indicate functional activity on the part of the graft. The effectiveness of the type of graft was assessed by the proportion of 'takes' within a group, by the average latent interval before the appearance of vaginal cornification and by the functional survival time of the graft. 4. The proportion of 'takes' was maximal in the autografts and intra-strain homografts, and only slightly less (16/20) in the inter-strain homografts. The length of the latent interval was not significantly different with the six types of graft. After 12–13 months, when the animals were killed, all the autografts, 11/14 of the intra-strain homografts, and 7/18 of the inter-strain homografts were still functioning. Of the thirty-six homografts which became established sufficiently to cause vaginal cornification, nine failed during the 1st month and three only during the 2nd and 3rd months. There were no later failures. 5. Histologically, most of the grafts were characterized by large follicular or lutein cysts, but normal eggs, follicles and corpora lutea were found in grafts of each type. There was a close connexion between the occurrence of follicular cysts and of persistent vaginal cornification. 6. The comparative importance of immunological and endocrinological factors in the evolution of the ovarian homograft is discussed.

scholarly journals METABOLIC AND ULTRASTRUCTURAL CHANGES IN THE FROG OVARIAN FOLLICLE IN RESPONSE TO PITUITARY STIMULATION

1970 ◽  
Vol 46 (3) ◽  
pp. 491-504 ◽  
Author(s):  
John Walberg Anderson ◽  
Milton B. Yatvin
Keyword(s):  
Epithelial Cells ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Ovarian Tissue ◽  
Ovarian Follicle ◽  
Ovarian Follicles ◽  
Ultrastructural Changes ◽  
Surface Epithelium

Frog ovarian fragments were prevented from ovulating in vitro by the addition of actinomycin D up to 3 hr following pituitary stimulation; but addition of Actinomycin D 6 hr after stimulation was far less effective. Puromycin, on the other hand, effectively inhibited ovulation when added as late as 6 hr after pituitary stimulation. Although actinomycin D reduced uptake of uridine-3H, and puromycin reduced uptake of leucine-3H and lysine-14 by pituitary-stimulated ovarian tissue minus oocytes (OTMO) in vitro, it was found that pituitary stimulation did not significantly increase uptake of these compounds by OTMO. Radioautographs of ovarian follicles fixed 6 hr after the addition of pituitary extract and uridine-3H in vitro revealed increased RNA synthesis in the peritoneal surface epithelium, compared with unstimulated controls, while the ovarian sac epithelium showed no increase. Gross ultrastructural changes occurred in the peritoneal area of ovarian follicles following pituitary stimulation in vivo, including loss of collagen fibrils, and general disorganization of the connective tissue theca. Changes in the rough endoplasmic reticulum of the peritoneal epithelial cells, while frequently encountered, were less pronounced. None of these changes was observed in the ovarian sac area, or in the interfollicular region. The above data are consistent with the hypothesis that pituitary stimulation of the frog ovary results in increased synthesis of RNA and protein by the peritoneal epithelial cells, and that the protein may be collagenase.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

Basal Lamina Formation in DMBA Induced Mammary Carcinoma

1977 ◽  
Vol 35 ◽  
pp. 534-535
Author(s):  
I. Russo ◽  
J. Saby ◽  
J. Russo
Keyword(s):  
Mammary Carcinoma ◽  
Virgin Female ◽  
Mammary Glands ◽  
Sesame Oil ◽  
Female Rats ◽  
Ultrastructural Changes ◽  
Post Inoculation ◽  
Intermediate Type ◽  
Sprague Dawley ◽  
Intercellular Spaces

It has been previously demonstrated that DMBA-induced rat mammary carcinoma originates in the terminal end bud (TEB) of the mammary gland by proliferation of intermediate type cells (1). The earliest lesion identified is the intraductal proliferation (IDP), which gives rise to intraductal carcinomas. These evolve to cribriform, papillary and comedo types (2). In the present work, we report the ultrastructural changes that take place in the IDP for the formation of a cribriform pattern.Fifty-five-day-old Sprague Dawley virgin female rats were inoculated intra- gastrically with 20 mg 7,12-dimethylbenz(a)anthracene (DMBA) in 1 ml sesame oil. Non-inoculated, age-matched females were used as controls. Mammary glands from both control and experimental rats were removed weekly from the time of inoculation until 86 days post-inoculation. The glands were fixed and processed for electron microscopy (2).The first change observed in IDP's was the widening of intercellular spaces and the secretion of an electron dense material into these spaces (Fig. 1).

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

Ultrastructural Changes in Patients With Heat Stroke

1970 ◽  
Vol 28 ◽  
pp. 210-211
Author(s):  
O. T. Minick ◽  
M. C. Kew
Keyword(s):  
Liver Damage ◽  
Heat Stroke ◽  
Free State ◽  
Ultrastructural Changes ◽  
Gold Mines

The effects of heat stroke on hepatic structure were studied in 32 Bantu patients who worked underground in the Transvaal and Orange Free State Gold Mines.Judging from biochemical and morphologic findings, liver damage is an invariable complication of heat stroke. In the milder cases (90 per cent) raised enzyme levels, bromsulphalein retention, and increased prothrombin times were the most common abnormalities.

Fine Structural Alterations in Thyroid Follicular Cells (FC) and Changes in Serum Thyroxine Produced by Feeding Polychlorinated Biphenyl (PCB) to Rats

1977 ◽  
Vol 35 ◽  
pp. 498-499
Author(s):  
W.T. Collins ◽  
Charles C. Capen ◽  
Louis Kasza
Keyword(s):  
Feed Efficiency ◽  
Polychlorinated Biphenyl ◽  
Serum Proteins ◽  
Skin Lesions ◽  
Hepatic Necrosis ◽  
Ultrastructural Changes ◽  
Lymphoid Tissues ◽  
Heat Transfer Fluids ◽  
Thyroid Follicular Cells ◽  
Peripheral Metabolism

The widespread contamination of the environment with PCB, a compound used extensively by industry in hydraulic and heat transfer fluids as well as plasticizers and solvents in adhesives and sealants, has resulted in detectable tissue levels in a large portion of the human population, domestic animals, and wildlife. Intoxication with PCB produces severe hepatic necrosis, degeneration of lymphoid tissues and kidney, skin lesions, decreased reproductive performance, reduced feed efficiency, and decreased weight gain. PCB also has been reported to reduce the binding of thyroid hormone to serum proteins and enhance the peripheral metabolism of thyroxine with increased excretion of thyroxine-glucuronide in the bile (Bastomsky, Endocrinology 95: 1150-1155, 1974).The objectives of this investigation were (1) to investigate the histopathologic, histochemical, and ultrastructural changes in thyroid FC produced by the acute (4 week) and chronic (12 week) administration of low (50 ppm) and high (500 ppm) doses of PCB to rats, (2) to correlate these alterations to changes in serum immunoreactive thyroxine concentration, and (3) to investigate the persistence of the effects of PCB on the thyroid gland.

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