Calcofluor white hypersensitive proteins contribute to stress tolerance and pathogenicity in entomopathogenic fungus, Metarhizium acridum

2020 ◽  
Author(s):  
Xueling Su ◽  
Xi Yan ◽  
Xing Chen ◽  
Mei Guo ◽  
Yuxian Xia ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Entomopathogenic Fungus ◽  
Calcofluor White ◽  
Metarhizium Acridum

scholarly journals The adenylate cyclase gene MaAC is required for virulence and multi-stress tolerance of Metarhizium acridum

2012 ◽  
Vol 12 (1) ◽  
pp. 163 ◽  
Author(s):  
Shuyang Liu ◽  
Guoxiong Peng ◽  
Yuxian Xia
Keyword(s):  
Adenylate Cyclase ◽  
Stress Tolerance ◽  
Metarhizium Acridum

scholarly journals MaAreB, a GATA Transcription Factor, Is Involved in Nitrogen Source Utilization, Stress Tolerances and Virulence in Metarhizium acridum

2021 ◽  
Vol 7 (7) ◽  
pp. 512
Author(s):  
Chaochuang Li ◽  
Qipei Zhang ◽  
Yuxian Xia ◽  
Kai Jin
Keyword(s):  
Cell Wall ◽  
Transcription Factor ◽  
Functional Characterization ◽  
Turgor Pressure ◽  
Nitrogen Utilization ◽  
Cell Wall Integrity ◽  
Homologous Proteins ◽  
Calcofluor White ◽  
Metarhizium Acridum ◽  
Gata Transcription Factor

The nitrogen catabolite repression (NCR) pathway is involved in nitrogen utilization, in which the global GATA transcription factor AreA plays an indispensable role and has been reported in many fungi. However, relatively few studies are focused on AreB, another GATA transcription factor in the NCR pathway and the functions of AreB are largely unknown in entomopathogenic fungi. Here, we characterized MaAreB in the model entomopathogenic fungus Metarhizium acridum. Sequence arrangement found that MaAreB had a conserved GATA zinc finger DNA binding domain and a leucine zipper domain. Disruption of MaAreB affected the nitrogen utilization and led to decelerated conidial germination and hyphal growth, decreased conidial yield, and lower tolerances to UV-B irradiation and heat-shock. Furthermore, the MaAreB mutant (ΔMaAreB) exhibited increased sensitivity to CFW (Calcofluor white), decreased cell wall contents (chitin and β-1,3-glucan) and reduced expression levels of some genes related to cell wall integrity, indicating that disruption of MaAreB affected the cell wall integrity. Bioassays showed that the virulence of the ΔMaAreB strain was decreased in topical inoculation but not in intra-hemocoel injection. Consistently, deletion of MaAreB severely impaired the appressorium formation and reduced the turgor pressure of appressorium. These results revealed that MaAreB regulated fungal nitrogen utilization, cell wall integrity and biological control potential, which would contribute to the functional characterization of AreB homologous proteins in other insect fungal pathogens, and even filamentous fungi.

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