Effects of extracts ofZanthoxylum fruit and their constituents on spontaneous beating rate of myocardial cell sheets in culture

Movement of individual cells or of cell sheets and complex patterns of folding play a prominent role in the early developmental stages of the embryo. Our understanding of these processes is based on three- dimensional reconstructions laboriously prepared from serial sections, and from autoradiographic and other studies. Many concepts have also evolved from extrapolation of investigations of cell movement carried out in vitro. The scanning electron microscope now allows us to examine some of these events in situ. It is possible to prepare dissections of embryos and even of tissues of adult animals which reveal existing relationships between various structures more readily than used to be possible vithout an SEM.

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A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100060672 ◽

1967 ◽

Vol 25 ◽

pp. 132-133

Author(s):

R. Stephens ◽

G. Schidlovsky ◽

S. Kuzmic ◽

P. Gaudreau

Keyword(s):

Electron Microscopy ◽

Tissue Culture ◽

Light Microscopy ◽

Cell Sheet ◽

Culture Cell ◽

Tissue Culture Cell ◽

Electron Microscopic ◽

Cell Sheets ◽

Morphological Alterations ◽

Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

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1739: Urothelium Regeneration Using Viable Cultured Urothelial Cell Sheets Grafted on Demucosalized Gastric Flaps

The Journal of Urology ◽

10.1016/s0022-5347(18)38931-6 ◽

2004 ◽

Vol 171 (4S) ◽

pp. 460-460

Author(s):

Yoshiyuki Shiroyanagi ◽

Masayuki Yamato ◽

Yuichiro Yamazaki ◽

Teruo Okano ◽

Hiroshi Toma

Keyword(s):

Urothelial Cell ◽

Cell Sheets

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Melatonin actions in the heart; more than a hormone

Melatonin Research ◽

10.32794/mr11250002 ◽

2018 ◽

Vol 1 (1) ◽

pp. 21-26 ◽

Cited By ~ 9

Author(s):

Darío Acuña-Castroviejo ◽

Maria T Noguiera-Navarro ◽

Russel J Reiter ◽

Germaine Escames

Keyword(s):

Cell Membranes ◽

Myocardial Cell ◽

Rat Tissues ◽

Dependent Manner ◽

Broad Distribution ◽

Multiple Organs ◽

High Doses ◽

Extrapineal Melatonin ◽

Dose Dependent ◽

Different Doses

Due to the broad distribution of extrapineal melatonin in multiple organs and tissues, we analyzed the presence and subcellular distribution of the indoleamine in the heart of rats. Groups of sham-operated and pinealectomized rats were sacrificed at different times along the day, and the melatonin content in myocardial cell membranes, cytosol, nuclei and mitochondria, were measured. Other groups of control animals were treated with different doses of melatonin to monitor its intracellular distribution. The results show that melatonin levels in the cell membrane, cytosol, nucleus, and mitochondria vary along the day, without showing a circadian rhythm. Pinealectomized animals trend to show higher values than sham-operated rats. Exogenous administration of melatonin yields its accumulation in a dose-dependent manner in all subcellular compartments analyzed, with maximal concentrations found in cell membranes at doses of 200 mg/kg bw melatonin. Interestingly, at dose of 40 mg/kg b.w, maximal concentration of melatonin was reached in the nucleus and mitochondrion. The results confirm previous data in other rat tissues including liver and brain, and support that melatonin is not uniformly distributed in the cell, whereas high doses of melatonin may be required for therapeutic purposes.

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STUDIES ON OESTROGENS IN CATTLE

Acta Endocrinologica ◽

10.1530/acta.0.xxxiv0027 ◽

1960 ◽

Vol XXXIV (I) ◽

pp. 27-32 ◽

Cited By ~ 6

Author(s):

Stian Erichsen ◽

Weiert Velle

Keyword(s):

Bovine Liver ◽

Liver Cells ◽

Cell Sheets ◽

Confluent Cell

ABSTRACT The metabolism of some oestrogenic hormones was studied in vitro by the use of cells grown on a medium free from blood. The methods used for the culture of cells from bovine testis, endometrium, amnion, and liver in confluent cell sheets on glass are described. The interconversion of oestrone and oestradiol-17β was demonstrated in the presence of cells from amnion, endometrium, and also from testicles of young calves and bulls. Only trace amounts of oestrone were found following incubations with oestradiol-17α in these tissues. Bovine liver cells grown in vitro showed a very poor capacity to bring about the interconversion mentioned above.

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Liver bud transplantation in rats

Magyar Sebészet (Hungarian Journal of Surgery) ◽

10.1556/1046.71.2018.4.3 ◽

2018 ◽

Vol 71 (4) ◽

pp. 163-169

Author(s):

Eiji Kobayashi ◽

Shin Enosawa

Keyword(s):

Fetal Liver ◽

Liver Parenchyma ◽

Arterial Blood Flow ◽

Silk Suture ◽

Cell Sheets ◽

Human Liver Tissue ◽

Arterial Blood ◽

Matrix Cell ◽

Experimental Microsurgery

Abstract: Introduction: Research has made progress in organ fabrication using an extracellular matrix, cell sheets, or organoids. Human liver tissue has been constructed using a 3-dimensional (3D) bioprinter and showed evidence that an in vitro generated liver bud was reformed in a rodent liver model. This study describes the stages of development of rat fetal organs and liver structure and reviews recent progress in liver organoid transplantation. Methods: The authors developed the procedures for creating a transected plane for use in experimental microsurgery in rats. A liver lobe was fixed vertically with gauze and it was ligated with 6-0 silk suture in the cut line; the parenchyma was cut, and major vessels were ligated to create the transected plane. The ligated tissue was carefully resected. Hemostasis was not required and hepatic components remained on the transected plane. The plane was covered by omentum. Results: Using this model, we transplanted fetal liver or a 3D bioprinted liver organoid. This microsurgical method enabled creation of an intact liver parenchyma plane. No bleeding was observed. The transplanted liver components successfully engrafted on the liver. Conclusion: This method may provide an essential environment for growing liver using portal and arterial blood flow.

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