Potential immunosuppressive and antiinflammatory activities of Malaysian medicinal plants characterized by reduced cell surface expression of cell adhesion molecules

2001 ◽  
Vol 15 (8) ◽  
pp. 681-686 ◽  
Author(s):  
Shigeo Tanaka ◽  
Sakata Yoichi ◽  
Lixi Ao ◽  
Mariko Matumoto ◽  
Katsushi Morimoto ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Medicinal Plants ◽  
Cell Surface ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Surface Expression ◽  
Cell Surface Expression

Influence of Natural and Synthetic Compounds on Cell Surface Expression of Cell Adhesion Molecules, ICAM-1 and VCAM-1

Planta Medica ◽  
2001 ◽  
Vol 67 (2) ◽  
pp. 108-113 ◽  
Author(s):  
Shigeo Tanaka ◽  
Yoichi Sakata ◽  
Katsushi Morimoto ◽  
Yukihiro Tambe ◽  
Yoshihiko Watanabe ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Adhesion Molecules ◽  
Cell Adhesion Molecules ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Synthetic Compounds ◽  
Natural And Synthetic

A Cell-Based Adhesion Molecule Bioassay Detects Unexpected Properties of Plasma From Patients with Sickle Cell Disease with Documented Pulmonary Hypertension

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 2120-2120
Author(s):  
Antje Ask ◽  
Laurel G. Mendelsohn ◽  
Shoaib Alam ◽  
Alem Mehari ◽  
Caterina Minniti ◽  
...  
Keyword(s):  
Pulmonary Hypertension ◽  
Cell Adhesion ◽  
Cell Surface ◽  
Adhesion Molecules ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Heart Catheterization ◽  
Soluble Adhesion Molecules

Abstract Abstract 2120 Pulmonary hypertension (PH) is a common complication in adults with sickle cell disease (SCD) associated with early mortality. Several mechanistic pathways appear to be involved in PH in SCD, one of them being activation of pulmonary endothelium and increased adherence of circulation blood cells. In the past, levels of soluble adhesion molecules in the plasma of patients with SCD have been found to correlate with severity of pulmonary hypertension and risk of mortality. We investigated the association between endothelial-cell based adhesion molecules and markers of PH. We developed a new cell-based ELISA assay and evaluated the induction of cell surface expression of adhesion molecules on cultured microvascular endothelium cells by plasma from subjects with SCD who had undergone right heart catheterization. We found no difference in baseline Intercellular Cell Adhesion Molecule-1 (ICAM-1), Vascular Cell Adhesion Molecule-1 (VCAM-1) and P-selectin induction by SCD plasma compared to healthy controls. Surprisingly, we found an inverse relationship of cell surface VCAM-1 induction with diagnosis and severity of PH, as indicated by mean pulmonary artery pressure (mPAP) on right heart catheterization. Patients who fell into the upper quartile of VCAM-1 induction had mPAP of 27.6 ± 3.2 mmHg, compared to the middle two quartiles 32 ± 2.3 mmHg, and lower quartile 38.2 ± 4.0 mmHg, (p=0.034). The prevalence of abnormally high pulmonary vascular resistance (>2 standard deviations above the mean) in the high, medium or low VCAM-1 induction groups was 20%, 35% and 80%, respectively (p=0.0066). We also found statistically significant correlations of cell surface VCAM-1 to cardiac output, transpulmonary gradient, pulse pressure, Doppler echocardiography tricuspid regurgitation velocity (TRV) and a marker of systemic iron overload, serum ferritin. Induced cell surface VCAM-1 expression did not correlate significantly in the same subjects with the plasma level of soluble VCAM-1, a previously documented marker associated with high TRV. We found very similar patterns of induction of cell surface expression of P-selectin. These results indicate that the ability of plasma to induce cell surface expression of cell adhesion molecules is a new marker predictive of the diagnosis of catheterization-proven PH in SCD, but it is independent of the levels of the soluble ectodomains of these cell adhesion molecules. These results are consistent with recent publications in the cell adhesion molecule field indicating that independent inflammation-mediated mechanisms regulate adhesion molecule expression and its ectodomain shedding via sheddases. Our findings lead us to speculate that increased sheddase activity may contribute to the high levels of soluble adhesion molecules found in PH, simultaneously reducing the level of cell surface adhesion molecules. Future studies of sheddase activity in SCD PH would help to elucidate this interesting observation. Disclosures: No relevant conflicts of interest to declare.

Perfusion with sickle erythrocytes up-regulates ICAM-1 andVCAM-1 gene expression in cultured human endothelial cells

Blood ◽  
2000 ◽  
Vol 95 (10) ◽  
pp. 3232-3241 ◽  
Author(s):  
Yan-Ting Shiu ◽  
Mark M. Udden ◽  
Larry V. McIntire
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Cell Adhesion ◽  
Cell Surface ◽  
Sickle Cell ◽  
Cell Adhesion Molecules ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Human Endothelial Cells ◽  
Sickle Erythrocytes

Sickle cell anemia is characterized by periodic vasoocclusive crises. Increased adhesion of sickle erythrocytes to vascular endothelium is a possible contributing factor to vasoocclusion. This study determined the effect of sickle erythrocyte perfusion at a venous shear stress level (1 dyne/cm2) on endothelial cell (EC) monolayers. Sickle erythrocytes up-regulated intercellular adhesion molecule-1 (ICAM-1) gene expression in cultured human endothelial cells. This was accompanied by increased cell surface expression of ICAM-1 and also elevated release of soluble ICAM-1 molecules. Expression of vascular cell adhesion molecule-1 (VCAM-1) messenger RNA (mRNA) was also strikingly elevated in cultured ECs after exposure to sickle cell perfusion, although increases in membrane-bound and soluble VCAM-1 levels were small. The presence of cytokine interleukin-1β in the perfusion system enhanced the production of ICAM-1 and VCAM-1 mRNA, cell surface expression, and the concentrations of circulating forms. This is the first demonstration that sickle erythrocytes have direct effects on gene regulation in cultured human ECs under well-defined flow environments. The results suggest that perfusion with sickle erythrocytes increases the expression of cell adhesion molecules on ECs and stimulates the release of soluble cell adhesion molecules, which may serve as indicators of injury and/or activation of endothelial cells. The interactions between sickle red blood flow, inflammatory cytokines, and vascular adhesion events may render sickle cell disease patients vulnerable to vasoocclusive crises.

Perfusion with sickle erythrocytes up-regulates ICAM-1 andVCAM-1 gene expression in cultured human endothelial cells

Blood ◽  
2000 ◽  
Vol 95 (10) ◽  
pp. 3232-3241 ◽  
Author(s):  
Yan-Ting Shiu ◽  
Mark M. Udden ◽  
Larry V. McIntire
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Cell Adhesion ◽  
Cell Surface ◽  
Sickle Cell ◽  
Cell Adhesion Molecules ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Human Endothelial Cells ◽  
Sickle Erythrocytes

Abstract Sickle cell anemia is characterized by periodic vasoocclusive crises. Increased adhesion of sickle erythrocytes to vascular endothelium is a possible contributing factor to vasoocclusion. This study determined the effect of sickle erythrocyte perfusion at a venous shear stress level (1 dyne/cm2) on endothelial cell (EC) monolayers. Sickle erythrocytes up-regulated intercellular adhesion molecule-1 (ICAM-1) gene expression in cultured human endothelial cells. This was accompanied by increased cell surface expression of ICAM-1 and also elevated release of soluble ICAM-1 molecules. Expression of vascular cell adhesion molecule-1 (VCAM-1) messenger RNA (mRNA) was also strikingly elevated in cultured ECs after exposure to sickle cell perfusion, although increases in membrane-bound and soluble VCAM-1 levels were small. The presence of cytokine interleukin-1β in the perfusion system enhanced the production of ICAM-1 and VCAM-1 mRNA, cell surface expression, and the concentrations of circulating forms. This is the first demonstration that sickle erythrocytes have direct effects on gene regulation in cultured human ECs under well-defined flow environments. The results suggest that perfusion with sickle erythrocytes increases the expression of cell adhesion molecules on ECs and stimulates the release of soluble cell adhesion molecules, which may serve as indicators of injury and/or activation of endothelial cells. The interactions between sickle red blood flow, inflammatory cytokines, and vascular adhesion events may render sickle cell disease patients vulnerable to vasoocclusive crises.

Cytokine-induced cell surface expression of adhesion molecules in vascular endothelial cellsIn vitro

2001 ◽  
Vol 21 (1) ◽  
pp. 68-71 ◽  
Author(s):  
Chen Honghui ◽  
Liu Changqin ◽  
Sun Shenggang ◽  
Mei Yuanwu ◽  
Tong E’tang
Keyword(s):  
Cell Surface ◽  
Adhesion Molecules ◽  
Surface Expression ◽  
Cell Surface Expression ◽  
Vascular Endothelial

scholarly journals Clinical Mutations in the L1 Neural Cell Adhesion Molecule Affect Cell-Surface Expression

2000 ◽  
Vol 20 (15) ◽  
pp. 5696-5702 ◽  
Author(s):  
Hugh D. Moulding ◽  
Robert L. Martuza ◽  
Samuel D. Rabkin
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Neural Cell Adhesion Molecule ◽  
Surface Expression ◽  
Neural Cell ◽  
Cell Surface Expression ◽  
Neural Cell Adhesion

scholarly journals Identification of novel cell-adhesion molecules in peripheral nerves using a signal-sequence trap

2005 ◽  
Vol 2 (1) ◽  
pp. 27-38 ◽  
Author(s):  
IVO SPIEGEL ◽  
KONSTANTIN ADAMSKY ◽  
MENAHEM EISENBACH ◽  
YAEL ESHED ◽  
ADRIAN SPIEGEL ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Sciatic Nerve ◽  
Cell Surface ◽  
Adhesion Molecules ◽  
Schwann Cells ◽  
Cell Adhesion Molecules ◽  
Signal Sequence ◽  
Cdna Libraries ◽  
Rat Sciatic Nerve ◽  
Myelinated Nerves

The development and maintenance of myelinated nerves in the PNS requires constant and reciprocal communication between Schwann cells and their associated axons. However, little is known about the nature of the cell-surface molecules that mediate axon–glial interactions at the onset of myelination and during maintenance of the myelin sheath in the adult. Based on the rationale that such molecules contain a signal sequence in order to be presented on the cell surface, we have employed a eukaryotic-based, signal-sequence-trap approach to identify novel secreted and membrane-bound molecules that are expressed in myelinating and non-myelinating Schwann cells. Using cDNA libraries derived from dbcAMP-stimulated primary Schwann cells and 3-day-old rat sciatic nerve mRNAs, we generated an extensive list of novel molecules expressed in myelinating nerves in the PNS. Many of the identified proteins are cell-adhesion molecules (CAMs) and extracellular matrix (ECM) components, most of which have not been described previously in Schwann cells. In addition, we have identified several signaling receptors, growth and differentiation factors, ecto-enzymes and proteins that are associated with the endoplasmic reticulum and the Golgi network. We further examined the expression of several of the novel molecules in Schwann cells in culture and in rat sciatic nerve by primer-specific, real-time PCR and in situ hybridization. Our results indicate that myelinating Schwann cells express a battery of novel CAMs that might mediate their interactions with the underlying axons.

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