Rapid mass spectrometry analysis of a rectilinear ion trap by continuous secular frequency scanning

2017 ◽  
Vol 31 (12) ◽  
pp. 1031-1040 ◽  
Author(s):  
Xinming Huo ◽  
Jin Chen ◽  
Fei Tang ◽  
Tongtong Yao ◽  
Shiyun Piao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Trap ◽  
Secular Frequency ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
Frequency Scanning ◽  
Rapid Mass

scholarly journals A straightforward gel-free proteomics pipeline assisted by liquid isoelectric focusing (OFFGEL) and mass spectrometry analysis to study bovine meat proteome

2020 ◽  
pp. 108201322092914
Author(s):  
Enrique Sentandreu ◽  
Claudia Fuente-García ◽  
José L Navarro ◽  
Miguel A Sentandreu
Keyword(s):  
Mass Spectrometry ◽  
Isoelectric Focusing ◽  
Quantitative Data ◽  
Ion Trap ◽  
Three Dimensional ◽  
Mass Spectrometry Analysis ◽  
Liquid Chromatography Mass Spectrometry ◽  
Protein Fractionation ◽  
Spectrometry Analysis ◽  
Proteomic Approach

Bovine sarcoplasmic sub-proteome was studied through a straightforward gel-free pipeline supported by liquid isoelectric focusing (OFFGEL) protein fractionation coupled to liquid chromatography-mass spectrometry (LC-MS) analysis. Full-MS and data-dependent MS/MS analyses were simultaneously performed by a conventional three-dimensional ion-trap addressing targeted quantitative and untargeted qualitative research, respectively. There were unambiguously identified 47 proteins distributed along 12 OFFGEL fractions assayed. Regarding intermediate- and high-abundant peptides, bulky quantitative data processing performed by MZmine 2 freeware yielded a satisfactory linearity and coefficient of variation with r2 in the 0.95–0.99 range and about 25%, respectively. Up to 41 peptides from 20 identified proteins were relatively quantified throughout OFFGEL fractions. This reliable, flexible and affordable gel-free proteomic approach could be readily implemented by industry to improve quality assessment of protein-based food products.

Sample preparation and direct electrospray ionization on a tip column for rapid mass spectrometry analysis of complex samples

The Analyst ◽  
2012 ◽  
Vol 137 (19) ◽  
pp. 4593 ◽  
Author(s):  
Yun-Qing Huang ◽  
Jin-Qing You ◽  
Bi-Feng Yuan ◽  
Yu-Qi Feng
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Electrospray Ionization ◽  
Mass Spectrometry Analysis ◽  
Complex Samples ◽  
Spectrometry Analysis ◽  
Rapid Mass

Capillary electrophoresis-ion trap mass spectrometry analysis of Ziagen® and its phosphorylated metabolites

2003 ◽  
Vol 24 (18) ◽  
pp. 3160-3164 ◽  
Author(s):  
Zongwei Cai ◽  
Eliza N. Fung ◽  
Achintya K. Sinhababu
Keyword(s):  
Mass Spectrometry ◽  
Capillary Electrophoresis ◽  
Ion Trap ◽  
Mass Spectrometry Analysis ◽  
Ion Trap Mass Spectrometry ◽  
Spectrometry Analysis

Development of Thermal Dissociation Atmospheric Chemical Ionization Source for Rapid Mass Spectrometry Analysis of Ambient Samples

2011 ◽  
Vol 39 (2) ◽  
pp. 288-292 ◽  
Author(s):  
Jing HAN ◽  
Jian-Qiang LI ◽  
Xie ZHANG ◽  
Bin HU ◽  
Ming-Biao LUO ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Chemical Ionization ◽  
Thermal Dissociation ◽  
Mass Spectrometry Analysis ◽  
Ionization Source ◽  
Spectrometry Analysis ◽  
Chemical Ionization Source ◽  
Rapid Mass
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