Programmable Electrodeposition of Janus Alginate/Poly‐L‐Lysine/Alginate (APA) Microcapsules for High‐Resolution Cell Patterning and Compartmentalization

Small ◽  
2021 ◽  
pp. 2106363
Author(s):  
Zeyang Liu ◽  
Haochen Nan ◽  
Yike Jiang ◽  
Tao Xu ◽  
Xiaohua Gong ◽  
...  
Keyword(s):  
High Resolution ◽  
Cell Patterning ◽  
Resolution Cell

scholarly journals Method for fast staining and obtaining high-magnification and high-resolution cell images of Nicotiana benthamiana

2021 ◽  
Vol 27 (1) ◽  
pp. 181-188
Author(s):  
Yuanyuan Jiang ◽  
Jiangrong Peng ◽  
Yunpeng Cao ◽  
Zhiqiang Han ◽  
Ling Zhang ◽  
...  
Keyword(s):  
High Resolution ◽  
Nicotiana Benthamiana ◽  
High Magnification ◽  
Resolution Cell

scholarly journals Biofilm Lithography enables high-resolution cell patterning via optogenetic adhesin expression

2018 ◽  
Vol 115 (14) ◽  
pp. 3698-3703 ◽  
Author(s):  
Xiaofan Jin ◽  
Ingmar H. Riedel-Kruse
Keyword(s):  
Escherichia Coli ◽  
Cell Patterning ◽  
Biophysical Model ◽  
Microbial Consortia ◽  
Bacterial Biofilms ◽  
Surface Pretreatment ◽  
Resolution Cell ◽  
Stimulation Of

Bacterial biofilms represent a promising opportunity for engineering of microbial communities. However, our ability to control spatial structure in biofilms remains limited. Here we engineerEscherichia coliwith a light-activated transcriptional promoter (pDawn) to optically regulate expression of an adhesin gene (Ag43). When illuminated with patterned blue light, long-term viable biofilms with spatial resolution down to 25 μm can be formed on a variety of substrates and inside enclosed culture chambers without the need for surface pretreatment. A biophysical model suggests that the patterning mechanism involves stimulation of transiently surface-adsorbed cells, lending evidence to a previously proposed role of adhesin expression during natural biofilm maturation. Overall, this tool—termed “Biofilm Lithography”—has distinct advantages over existing cell-depositing/patterning methods and provides the ability to grow structured biofilms, with applications toward an improved understanding of natural biofilm communities, as well as the engineering of living biomaterials and bottom–up approaches to microbial consortia design.

scholarly journals High-Resolution Cell Surface Dynamics of Germinating Aspergillus fumigatus Conidia

2008 ◽  
Vol 94 (2) ◽  
pp. 656-660 ◽  
Author(s):  
Etienne Dague ◽  
David Alsteens ◽  
Jean-Paul Latgé ◽  
Yves F. Dufrêne
Keyword(s):  
High Resolution ◽  
Cell Surface ◽  
Aspergillus Fumigatus ◽  
Surface Dynamics ◽  
Resolution Cell

scholarly journals High resolution cell lineage tracing reveals developmental variability in leech

2009 ◽  
Vol 238 (12) ◽  
pp. 3139-3151 ◽  
Author(s):  
Stephanie E. Gline ◽  
Dian-Han Kuo ◽  
Alberto Stolfi ◽  
David A. Weisblat
Keyword(s):  
High Resolution ◽  
Cell Lineage ◽  
Lineage Tracing ◽  
Resolution Cell
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