Nucleolus-Targeted Red Emissive Carbon Dots with Polarity-Sensitive and Excitation-Independent Fluorescence Emission: High-Resolution Cell Imaging and in Vivo Tracking

2019 ◽  
Vol 11 (36) ◽  
pp. 32647-32658 ◽  
Author(s):  
Xian-Wu Hua ◽  
Yan-Wen Bao ◽  
Jia Zeng ◽  
Fu-Gen Wu
Keyword(s):  
High Resolution ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Fluorescence Emission ◽  
Polarity Sensitive ◽  
Resolution Cell

Biocompatible Carbon Dots Clusters with Tunable Sizes for Bioimaging Application

Nano LIFE ◽  
2016 ◽  
Vol 06 (01) ◽  
pp. 1640001
Author(s):  
Guan Wang ◽  
Junlong Geng ◽  
Ye Liu
Keyword(s):  
High Resolution ◽  
Renal Clearance ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Pluronic F127 ◽  
High Brightness ◽  
Engineering Strategy ◽  
Low Cytotoxicity

Carbon dots (Cdots) have been widely applied in labeling cellular or subcellular targets with high resolution due to their small sizes and benign biocompatibility. However, their use for in vivo application suffered from the rapid renal clearance due to their tiny size (c.a. [Formula: see text]6[Formula: see text]nm in diameter). To overcome the drawback, we herein report a sonication assisted method for the preparation of Cdots clusters with tunable sizes. Pluronic F127 was used as the carrier to form the clusters and the feeding ratio of the Cdots to F127 was varied in order to tune the sizes of clusters. The clusters were also successfully demonstrated in cell imaging applications with high brightness. Together with their low cytotoxicity, the clusters are potential for bioimaging applications. Hence, this work provides a convenient engineering strategy to prepare Cdots clusters with tunable sizes for bioimaging applications.

Green and Simple Synthesis of Photoluminescence-Tunable Carbon Dots for Sensing and Cell Imaging Applications

2021 ◽  
Vol 21 (12) ◽  
pp. 6101-6110
Author(s):  
Dong Sun ◽  
Shu-Jun Li ◽  
Chun-Feng Wang ◽  
Tian-Tian Liu ◽  
Guang-Yue Bai ◽  
...  
Keyword(s):  
Boric Acid ◽  
Carbon Dots ◽  
Luminescence Property ◽  
Cell Imaging ◽  
Fluorescence Emission ◽  
Fluorescent Imaging ◽  
Simple Synthesis ◽  
Low Toxicity ◽  
Doped Carbon Dots ◽  
Co Doped

Innovative nitrogen and boron co-doped carbon dots are hydrothermally produced using fructose, urea, and boric acid as precursors. The synthesized carbon dots possess a uniform morphology, and exhibit excellent fluorescence stability, tunable luminescence property, strong resistance to photobleaching, low-toxicity, and excellent biocompatibility. It is also found more dopant urea is conducive to the formation of the carbon dots with more B–N bonds, and shorter wavelength of fluorescence emission. Meanwhile, the synthesized carbon dots are well utilized as a photoluminescent probe for facile Hg2+ determination and fluorescent imaging reagent in cells.

Carbon dots of different composition and surface functionalization: cytotoxicity issues relevant to fluorescence cell imaging

2011 ◽  
Vol 236 (11) ◽  
pp. 1231-1238 ◽  
Author(s):  
Yanli Wang ◽  
Parambath Anilkumar ◽  
Li Cao ◽  
Jia-Hui Liu ◽  
Pengju G Luo ◽  
...  
Keyword(s):  
Surface Functionalization ◽  
Carbon Dots ◽  
Carbon Nanoparticles ◽  
Cell Imaging ◽  
Surface Passivation ◽  
Particle Surface ◽  
Live Cells ◽  
Carbon Particles

Nanoscale carbon particles have emerged as versatile precursors for a new class of highly fluorescent nanomaterials that resemble semiconductor quantum dots. The surface-passivated fluorescent carbon nanoparticles, dubbed ‘carbon dots’, were already demonstrated for their potential optical bioimaging applications in vitro and in vivo. In this study, we conducted a systematic cytotoxicity evaluation on the carbon dots prepared by various combinations of precursor carbon nanoparticles and molecules for the particle surface functionalization. The results suggested that the cytotoxicity of carbon dots was dependent on the selection of surface passivation molecules. Those dots showing more significant cytotoxicity at higher concentrations were also evaluated for their effects on the fluorescence imaging of live cells. The implications of the results on the eventual use of carbon dots as cell imaging agents are discussed.

scholarly journals Photoswitchable Nanoparticles Enable High-Resolution Cell Imaging: PULSAR Microscopy

2008 ◽  
Vol 130 (46) ◽  
pp. 15279-15281 ◽  
Author(s):  
Dehong Hu ◽  
Zhiyuan Tian ◽  
Wuwei Wu ◽  
Wei Wan ◽  
Alexander D. Q. Li
Keyword(s):  
High Resolution ◽  
Cell Imaging ◽  
Resolution Cell

Fluorescent Carbon Dots (CDs) as a pH Sensor

2011 ◽  
Vol 415-417 ◽  
pp. 1319-1322 ◽  
Author(s):  
Feng Lin ◽  
Wei Na He ◽  
Xiang Qun Guo
Keyword(s):  
Carbon Dots ◽  
Spectral Response ◽  
Strong Dependence ◽  
Ph Sensor ◽  
Fluorescence Emission ◽  
Intracellular Imaging ◽  
Green Material ◽  
Ph Dependent ◽  
Fluorescent Carbon Dots

The pH-dependent properties of carbon dots (CDs), decorated with surface carboxylic acid functional groups, are described. Absorption, fluorescence emission measurements were used to study the reversible pH-dependent spectral response. The pH-dependent emissions of CDs demonstrate a strong dependence on the excitation wavelengths and make the as-prepared CDs a good ratiometric pH-sensor candidate. This green material CDs-based probe shows promising potential for probing pH in real sample and in vivo intracellular imaging.

High photoluminescent nitrogen-doped carbon dots with unique double wavelength fluorescence emission for cell imaging

2018 ◽  
Vol 216 ◽  
pp. 84-87 ◽  
Author(s):  
Wenli Gao ◽  
Yiming Ma ◽  
Yanmei Zhou ◽  
Haohan Song ◽  
Li Li ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Cell Imaging ◽  
Fluorescence Emission ◽  
Nitrogen Doped ◽  
Doped Carbon Dots ◽  
Nitrogen Doped Carbon

scholarly journals Assurance of monoclonality in one round of cloning through cell sorting for single cell deposition coupled with high resolution cell imaging

2015 ◽  
Vol 31 (5) ◽  
pp. 1172-1178 ◽  
Author(s):  
Krista Evans ◽  
Thomas Albanetti ◽  
Raghavan Venkat ◽  
Ronald Schoner ◽  
James Savery ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Cell ◽  
Cell Sorting ◽  
Cell Imaging ◽  
Resolution Cell
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