scholarly journals High‐Speed Atomic Force Microscopy: Large‐Range HS‐AFM Imaging of DNA Self‐Assembly through In Situ Data‐Driven Control (Small Methods 7/2019)

Small Methods ◽  
2019 ◽  
Vol 3 (7) ◽  
pp. 1970022
Author(s):  
Adrian P. Nievergelt ◽  
Christoph Kammer ◽  
Charlène Brillard ◽  
Eva Kurisinkal ◽  
Maartje M. C. Bastings ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Self Assembly ◽  
High Speed ◽  
Large Range ◽  
Data Driven ◽  
Force Microscopy ◽  
In Situ Data ◽  
Atomic Force ◽  
Afm Imaging

scholarly journals Large‐Range HS‐AFM Imaging of DNA Self‐Assembly through In Situ Data‐Driven Control

Small Methods ◽  
2019 ◽  
Vol 3 (7) ◽  
pp. 1900031 ◽  
Author(s):  
Adrian P. Nievergelt ◽  
Christoph Kammer ◽  
Charlène Brillard ◽  
Eva Kurisinkal ◽  
Maartje M. C. Bastings ◽  
...  
Keyword(s):  
Self Assembly ◽  
Large Range ◽  
Data Driven ◽  
In Situ Data ◽  
Afm Imaging

scholarly journals Quantitative comparison of wideband low-latency phase-locked loop circuit designs for high-speed frequency modulation atomic force microscopy

2018 ◽  
Vol 9 ◽  
pp. 1844-1855 ◽  
Author(s):  
Kazuki Miyata ◽  
Takeshi Fukuma
Keyword(s):  
Atomic Force Microscopy ◽  
Frequency Modulation ◽  
High Speed ◽  
Feedback Loop ◽  
Response Speed ◽  
Low Latency ◽  
Pass Filter ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

A phase-locked loop (PLL) circuit is the central component of frequency modulation atomic force microscopy (FM-AFM). However, its response speed is often insufficient, and limits the FM-AFM imaging speed. To overcome this issue, we propose a PLL design that enables high-speed FM-AFM. We discuss the main problems with the conventional PLL design and their possible solutions. In the conventional design, a low-pass filter with relatively high latency is used in the phase feedback loop, leading to a slow response of the PLL. In the proposed design, a phase detector with a low-latency high-pass filter is located outside the phase feedback loop, while a subtraction-based phase comparator with negligible latency is located inside the loop. This design minimizes the latency within the phase feedback loop and significantly improves the PLL response speed. In addition, we implemented PLLs with the conventional and proposed designs in the same field programmable gate array chip and quantitatively compared their performances. The results demonstrate that the performance of the proposed PLL is superior to that of the conventional PLL: 165 kHz bandwidth and 3.2 μs latency in water. Using this setup, we performed FM-AFM imaging of calcite dissolution in water at 0.5 s/frame with true atomic resolution. The high-speed and high-resolution imaging capabilities of the proposed design will enable a wide range of studies to be conducted on various atomic-scale dynamic phenomena at solid–liquid interfaces.

scholarly journals In Situ Atomic Force Microscopy Studies on Nucleation and Self-Assembly of Biogenic and Bio-Inspired Materials

Minerals ◽  
2017 ◽  
Vol 7 (9) ◽  
pp. 158 ◽  
Author(s):  
Cheng Zeng ◽  
Caitlin Vitale-Sullivan ◽  
Xiang Ma
Keyword(s):  
Atomic Force Microscopy ◽  
Self Assembly ◽  
Force Microscopy ◽  
Atomic Force

In situ studies of thiol self-assembly on gold from solution using atomic force microscopy

1998 ◽  
Vol 108 (12) ◽  
pp. 5002-5012 ◽  
Author(s):  
Song Xu ◽  
Sylvain J. N. Cruchon-Dupeyrat ◽  
Jayne C. Garno ◽  
Gang-Yu Liu ◽  
G. Kane Jennings ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Self Assembly ◽  
Force Microscopy ◽  
In Situ Studies ◽  
Atomic Force

scholarly journals Choosing a Cantilever for In Situ Atomic Force Microscopy

2003 ◽  
Vol 11 (2) ◽  
pp. 42-43
Author(s):  
John T. Woodward
Keyword(s):  
Biological Sciences ◽  
Atomic Force Microscopy ◽  
Biological Samples ◽  
Contact Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging ◽  
Interesting Discussion ◽  
Intermittent Contact

What is the best cantilever for intermittent contact mode (often called Tapping Mode™) atomic force microscope (AFM) imaging under water? This is a question I hear often and one that recently generated some interesting discussion on an AFM newsgroup (more on the newsgroup below). The ability of the AFM to image samples En physiologically relevant environments has made it a popular technique in the biological sciences. However, because scanning the AFM tip in contact mode easily perturbs many biological samples, it was the advent of intermittent contact modes that lead to AFM's widespread use in biology.

scholarly journals Self-assembly of influenza hemagglutinin: studies of ectodomain aggregation by in situ atomic force microscopy

2001 ◽  
Vol 1513 (2) ◽  
pp. 167-175 ◽  
Author(s):  
Raquel F. Epand ◽  
Christopher M. Yip ◽  
Leonid V. Chernomordik ◽  
Danika L. LeDuc ◽  
Yeon-Kyun Shin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Self Assembly ◽  
Force Microscopy ◽  
Influenza Hemagglutinin ◽  
Atomic Force

scholarly journals Structure of Intracellular Mature Vaccinia Virus Visualized by In Situ Atomic Force Microscopy

2003 ◽  
Vol 77 (11) ◽  
pp. 6332-6340 ◽  
Author(s):  
A. J. Malkin ◽  
A. McPherson ◽  
P. D. Gershon
Keyword(s):  
Atomic Force Microscopy ◽  
Vaccinia Virus ◽  
Helical Surface ◽  
Force Microscopy ◽  
Virus Particles ◽  
The Core ◽  
Atomic Force ◽  
Afm Imaging ◽  
Intact Core

ABSTRACT Vaccinia virus, the basis of the smallpox vaccine, is one of the largest viruses to replicate in humans. We have used in situ atomic force microscopy (AFM) to directly visualize fully hydrated, intact intracellular mature vaccinia virus (IMV) virions and chemical and enzymatic treatment products thereof. The latter included virion cores, core-enveloping coats, and core substructures. The isolated coats appeared to be composed of a highly cross-linked protein array. AFM imaging of core substructures indicated association of the linear viral DNA genome with a segmented protein sheath forming an extended ∼16-nm-diameter filament with helical surface topography; enclosure of this filament within a 30- to 40-nm-diameter tubule which also shows helical topography; and enclosure of the folded, condensed 30- to 40-nm-diameter tubule within the core by a wall covered with peg-like projections. Proteins observed attached to the 30- to 40-nm-diameter tubules may mediate folding and/or compaction of the tubules and/or represent vestiges of the core wall and/or pegs. An accessory “satellite domain” was observed protruding from the intact core. This corresponded in size to isolated 70- to 100-nm-diameter particles that were imaged independently and might represent detached accessory domains. AFM imaging of intact virions indicated that IMV underwent a reversible shrinkage upon dehydration (as much as 2.2- to 2.5-fold in the height dimension), accompanied by topological and topographical changes, including protrusion of the satellite domain. As shown here, the chemical and enzymatic dissection of large, asymmetrical virus particles in combination with in situ AFM provides an informative complement to other structure determination techniques.

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