scholarly journals Engineering of Dominant Active Basic Helix-Loop-Helix Proteins That Are Resistant to Negative Regulation by Postnatal Central Nervous System Antineurogenic Cues

Stem Cells ◽  
2009 ◽  
Vol 27 (4) ◽  
pp. 847-856 ◽  
Author(s):  
Cédric G. Geoffroy ◽  
James A. Critchley ◽  
Diogo S. Castro ◽  
Sandra Ramelli ◽  
Christelle Barraclough ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Negative Regulation ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

scholarly journals Helt, a Novel Basic-Helix-Loop-Helix Transcriptional Repressor Expressed in the Developing Central Nervous System

2004 ◽  
Vol 279 (16) ◽  
pp. 16356-16367 ◽  
Author(s):  
Tomoya Nakatani ◽  
Eri Mizuhara ◽  
Yasuko Minaki ◽  
Yoshimasa Sakamoto ◽  
Yuichi Ono
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Transcriptional Repressor ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

scholarly journals Motor neurons involved in fine motor control are labeled by tracing Atoh1-lineage neurons in the spinal cord

2020 ◽  
Author(s):  
Osita W. Ogujiofor ◽  
Iliodora V. Pop ◽  
Felipe Espinosa ◽  
Razaq O. Durodoye ◽  
Michael L. Viacheslavov ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Motor Neurons ◽  
Fine Motor ◽  
Bhlh Transcription Factor ◽  
Mouse Line ◽  
Developmental History ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
The Central Nervous System

AbstractMotor neurons (MNs) innervating the digit muscles of the intrinsic hand and foot (IH and IF) control fine motor movements. Previous studies suggest that the IH and IF MN pools have a unique developmental history in comparison to limb MN pools. Consistent with having this unique development, we find that the IH and IF MN pools are labeled postnatally using a CRE knock-in mouse line of Atoh1, a developmentally expressed basic helix-loop-helix (bHLH) transcription factor, while limb-innervating MN pools are not. Approximately 60% of the IH and IF MN pools are labeled and are a mixture of alpha and gamma-MNs. In addition, because Atoh1 is known developmentally to specify many cerebellar-projecting neurons, we tested the hypothesis that IH and IF MNs can send axon collaterals to the cerebellum as a mechanism of corollary discharge. Using intersectional genetic, viral labeling, and retrograde labeling strategies, we were unable to provide evidence in support of this idea. As a secondary finding of our viral labeling experiments, we report here that injection of both AAV and Lentiviruses in the periphery can cross the blood-brain barrier to infect Purkinje cells within the central nervous system. Altogether, though, we find that labeling of the IH and IF motor neurons using the Atoh1 CRE knock-in mouse suggests that IH and IF MNs have a unique developmental history and that this mouse strain might be a useful tool to target these specific sets of neurons allowing for functional studies of fine motor control.Significance StatementMotor neurons (MNs) of the intrinsic hand and foot (IH and IF) are labeled postnatally using a CRE knock-in mouse line of the basic helix-loop-helix (bHLH) transcription factor Atoh1 indicating a unique developmental history. We tested whether IH and IF MNs send axon collaterals rostrally to the cerebellum as a mechanism of direct corollary discharge from MNs, but the question remains unresolved. As a resource for the community, we report that injection of both AAV and Lentiviruses in the periphery can cross the blood-brain barrier and infect Purkinje cells within the central nervous system.

scholarly journals Persistent expression of helix-loop-helix factor HES-1 prevents mammalian neural differentiation in the central nervous system.

1994 ◽  
Vol 13 (8) ◽  
pp. 1799-1805 ◽  
Author(s):  
M. Ishibashi ◽  
K. Moriyoshi ◽  
Y. Sasai ◽  
K. Shiota ◽  
S. Nakanishi ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Neural Differentiation ◽  
Helix Loop Helix ◽  
The Central Nervous System

scholarly journals Characterization of Npas3 , a novel basic helix-loop-helix PAS gene expressed in the developing mouse nervous system

1999 ◽  
Vol 88 (2) ◽  
pp. 237-241 ◽  
Author(s):  
Eric W Brunskill ◽  
David P Witte ◽  
Andrew B Shreiner ◽  
S.Steven Potter
Keyword(s):  
Nervous System ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix

scholarly journals Control of CNS midline transcription by asymmetric E-box-like elements: similarity to xenobiotic responsive regulation

Development ◽  
1994 ◽  
Vol 120 (12) ◽  
pp. 3563-3569 ◽  
Author(s):  
K.A. Wharton ◽  
R.G. Franks ◽  
Y. Kasai ◽  
S.T. Crews
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Germ Line ◽  
Embryonic Cells ◽  
Helix Loop Helix ◽  
E Box ◽  
Cns Midline ◽  
Midline Cells ◽  
Separate Identity ◽  
Xenobiotic Response

Central nervous system midline cells constitute a discrete group of Drosophila embryonic cells with numerous functional and developmental roles. Corresponding to their separate identity, the midline cells display patterns of gene expression distinct from the lateral central nervous system. A conserved 5 base pair sequence (ACGTG) was identified in central nervous system midline transcriptional enhancers of three genes. Germ-line transformation experiments indicate that this motif forms the core of an element required for central nervous system midline transcription. The central nervous system midline element is related to the mammalian xenobiotic response element, which regulates transcription of genes that metabolize aromatic hydrocarbons. These data suggest a model whereby related basic-helix-loop-helix-PAS proteins interact with asymmetric E-box-like target sequences to control these disparate processes.

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