Licochalcone A induces apoptotic cell death via JNK/p38 activation in human nasopharyngeal carcinoma cells

2019 ◽  
Vol 34 (7) ◽  
pp. 853-860 ◽  
Author(s):  
Chun‐Yi Chuang ◽  
Cheng‐Ming Tang ◽  
Hsin‐Yu Ho ◽  
Chung‐Han Hsin ◽  
Chia‐Jui Weng ◽  
...  
Keyword(s):  
Cell Death ◽  
Nasopharyngeal Carcinoma ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Carcinoma Cells ◽  
Licochalcone A ◽  
Human Nasopharyngeal Carcinoma

Bufalin Induces Apoptotic Cell Death in Human Nasopharyngeal Carcinoma Cells through Mitochondrial ROS and TRAIL Pathways

2019 ◽  
Vol 47 (01) ◽  
pp. 237-257 ◽  
Author(s):  
En-Yun Su ◽  
Yung-Lin Chu ◽  
Fu-Shin Chueh ◽  
Yi-Shih Ma ◽  
Shu-Fen Peng ◽  
...  
Keyword(s):  
Cell Death ◽  
Nasopharyngeal Carcinoma ◽  
Er Stress ◽  
Apoptotic Cell ◽  
Morphological Changes ◽  
Chromatin Condensation ◽  
Apoptotic Cell Death ◽  
Dapi Staining ◽  
Human Nasopharyngeal Carcinoma ◽  
Associated Proteins

The aim of this study was to investigate the effects of bufalin on human nasopharyngeal carcinoma NPC-TW 076 cells in vitro. Bufalin is a cardiotonic steroid and a key active ingredient of the Chinese medicine ChanSu. The extracts of Chansu are used for various cancer treatments in China. In the present study, bufalin induced cell morphological changes, decreased total cell viability and induced G2/M phase arrest of cell cycle in NPC-TW 076 cells. Results also indicated that bufalin induced chromatin condensation (cell apoptosis) and DNA damage by DAPI staining and comet assay, respectively. The induced apoptotic cell death was further confirmed by annexin-V/PI staining assay. In addition, bufalin also increased ROS and Ca[Formula: see text] production and decreased the levels of [Formula: see text]. Furthermore, the alterations of ROS, ER stress and apoptosis associated protein expressions were investigated by Western blotting. Results demonstrated that bufalin increased the expressions of ROS associated proteins, including SOD (Cu/Zn), SOD2 (Mn) and GST but decreased that of catalase. Bufalin increased ER stress associated proteins (GRP78, IRE-1[Formula: see text], IRE-1[Formula: see text], caspase-4, ATF-6[Formula: see text], Calpain 1, and GADD153). Bufalin increased the pro-apoptotic proteins Bax, and apoptotic associated proteins (cytochrome c, caspase-3, -8 and -9, AIF and Endo G) but reduced anti-apoptotic protein Bcl-2 in NPC-TW 076 cells. Furthermore, bufalin elevated the expressions of TRAIL-pathway associated proteins (TRAIL, DR4, DR5, and FADD). Based on these findings, we suggest bufalin induced apoptotic cell death via caspase-dependent, mitochondria-dependent and TRAIL pathways in human nasopharyngeal carcinoma NPC-TW 076 cells.

scholarly journals Mechanism of apoptotic cell death of human gastric carcinoma cells mediated by transforming growth factor β

1997 ◽  
Vol 324 (3) ◽  
pp. 777-782 ◽  
Author(s):  
Shigeki OHTA ◽  
Kazuyoshi YANAGIHARA ◽  
Kiyoshi NAGATA
Keyword(s):  
Cell Death ◽  
Gastric Carcinoma ◽  
Expression Vector ◽  
Transforming Growth Factor ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Carcinoma Cells ◽  
Human Gastric Carcinoma ◽  
Human Gastric Carcinoma Cells ◽  
Tgf Β1

Human gastric carcinoma cell line HSC-39 has been shown to undergo apoptotic cell death in response to treatment with transforming growth factor β1 (TGF-β1). To understand better the cell death mechanism in this TGF-β1-mediated apoptosis, we investigated the effect of the expression of TGF-β-stimulated clone 22 (TSC-22) on cell death events. TGF-β1 induced TSC-22 gene expression in HSC-39 cells only when the cells had previously been adapted to the serum-free culture conditions required to undergo TGF-β1-mediated apoptosis. HSC-39 cells transfected with a TSC-22 expression vector showed a significant decrease in cell viability compared with those transfected with a control vector. The cellular events characteristic of apoptosis, chromatin condensation and DNA fragmentation were observed only in cells transfected with a TSC-22 expression vector. On immunostaining of the transfected cells, almost every cell that expressed TSC-22 tagged with influenza virus haemagglutinin exhibited the morphology of an apoptotic cell. Partial protection from the cell death effect of TGF-β1 on HSC-39 cells was observed when cells were treated with acetyl-l-aspartyl-l-glutamyl-l-valyl-l-aspart-1-al (Ac-DEVD-CHO, an inhibitor specific for CPP32-type protease). Protection against cell death by the transfection of a TSC-22 expression vector was also offered by Ac-DEVD-CHO addition. These results suggest that TSC-22 elicits the apoptotic cell death of human gastric carcinoma cells through the activation of CPP32-like protease and mediates the TGF-β1 signalling pathway to apoptosis.

scholarly journals Novel oncolytic adenovirus sensitizes renal cell carcinoma cells to radiotherapy via mitochondrial apoptotic cell death

2014 ◽  
Vol 11 (3) ◽  
pp. 2141-2146 ◽  
Author(s):  
REN-FU CHEN ◽  
YUE-YAN LI ◽  
LIAN-TAO LI ◽  
QIAN CHENG ◽  
GUAN JIANG ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Death ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Apoptotic Cell ◽  
Oncolytic Adenovirus ◽  
Apoptotic Cell Death ◽  
Carcinoma Cells

P-glycoprotein enhances radiation-induced apoptotic cell death through the regulation of miR-16 and Bcl-2 expressions in hepatocellular carcinoma cells

APOPTOSIS ◽  
2011 ◽  
Vol 16 (5) ◽  
pp. 524-535 ◽  
Author(s):  
Tsun Yee Tsang ◽  
Wan Yee Tang ◽  
Judy Yuet Wa Chan ◽  
Ngai Na Co ◽  
Chi Lam Au Yeung ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Carcinoma Cells ◽  
Hepatocellular Carcinoma Cells ◽  
P Glycoprotein ◽  
Radiation Induced

Valproic Acid Sensitizes TRAIL-Resistant Anaplastic Thyroid Carcinoma Cells to Apoptotic Cell Death

2013 ◽  
Vol 20 (S3) ◽  
pp. 716-724 ◽  
Author(s):  
Hyun-Young Cha ◽  
Bok-Soon Lee ◽  
Sam Kang ◽  
Yoo Seob Shin ◽  
Jae Won Chang ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Cell Death ◽  
Thyroid Carcinoma ◽  
Apoptotic Cell ◽  
Anaplastic Thyroid Carcinoma ◽  
Apoptotic Cell Death ◽  
Carcinoma Cells

scholarly journals XIAP inhibitor embelin induces autophagic and apoptotic cell death in human oral squamous cell carcinoma cells

2017 ◽  
Vol 32 (11) ◽  
pp. 2371-2378 ◽  
Author(s):  
You-Jin Lee ◽  
Bong-Soo Park ◽  
Hae-Ryoun Park ◽  
Su-Bin Yu ◽  
Hae-Mi Kang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Carcinoma Cells
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