Developing a non‐invasive method of detecting elephant endotheliotropic herpesvirus infections using faecal samples

2021 ◽  
Author(s):  
Sophie M. Common ◽  
Yaoprapa Yun ◽  
Ayona Silva‐Fletcher ◽  
Chatchote Thitaram ◽  
Thittaya Janyamethakul ◽  
...  
Keyword(s):  
Non Invasive ◽  
Faecal Samples ◽  
Invasive Method

scholarly journals Sex determination in gibbons of genus Nomascus using non-invasive method

2016 ◽  
Vol 85 (4) ◽  
pp. 363-366 ◽  
Author(s):  
Petra Bolechová ◽  
Kateřina Ječmínková ◽  
Michal Hradec ◽  
Tomáš Kott ◽  
Jana Doležalová
Keyword(s):  
Sex Determination ◽  
Direct Examination ◽  
Chain Reactions ◽  
Polymerase Chain Reactions ◽  
Non Invasive ◽  
Faecal Samples ◽  
Invasive Method ◽  
Polymerase Chain ◽  
Template Dna

Gibbons of the genus Nomascus have a strong sexual dimorphism and dichromatism. As they mature, both sexes develop sex-specific pelage colour. In combination with physical similarities in the genitalia with both sexes, there are problems with determining the sex of young individuals compared to other genus of gibbons. This is a pilot study applying a multiplex polymerase chain reactions based on a non-invasive method for sex determination of gibbons. The study was conducted on 22 faecal samples from gibbons of the genus Nomascus. The animals were monitored by staff so that the samples were identified correctly and each sample was collected immediately after the defecation. Results confirmed the sex in all adult and juvenile animals with known sex; and 2 females and 5 males in juveniles were determined with unknown sex. The results of direct examination completely corresponded with the PCR results. The PCR reaction with template DNA isolated from faecal material required BSA usage, however, we observed the occurrence of nonspecific fragments. This did not affect the reliability of our results and we confirmed the usability of this method for this genus.

scholarly journals X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats

2017 ◽  
Vol 65 (4) ◽  
pp. 500-504 ◽  
Author(s):  
Renáta Fábián ◽  
András Kovács ◽  
Viktor Stéger ◽  
Krisztián Frank ◽  
István Egerszegi ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Sex Reversal ◽  
Agarose Gel Electrophoresis ◽  
Amelogenin Gene ◽  
Non Invasive ◽  
Faecal Samples ◽  
Pcr Products ◽  
Invasive Method ◽  
Pcr Method ◽  
Male Sex

The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.

Measurement of immunoreactive progestins excreted in faeces as a potential indicator of pregnancy

1993 ◽  
Vol 20 (6) ◽  
pp. 739 ◽  
Author(s):  
NC Larter ◽  
P Arcese ◽  
R Rajamahendran ◽  
CC Gates
Keyword(s):  
Pregnancy Rate ◽  
Bison Bison ◽  
Non Invasive ◽  
Faecal Samples ◽  
Potential Indicator ◽  
Invasive Method ◽  
Wood Bison ◽  
Free Ranging

We measured the concentration of immunoreactive progestins extracted from 41 faecal samples of female wood bison (Bison bison athabascae) as a potential indicator of pregnancy. The concentration of faecal progestins fell into two distinct groups, with an approximate 4-fold difference in mean progestin level (1391.9ng g-1 and 58269ng g-1). We suggest that measuring progestin concentration in the faeces of free-ranging animals is a non-invasive method that could be used to assess pregnancy rate.

scholarly journals Diet of the Viviparous lizard Zootoca vivipara (Lichtenstein, 1823) (Reptilia: Lacertidae) from its southern range

2019 ◽  
Vol 2 ◽  
Author(s):  
Emiliya Vacheva ◽  
Borislav Naumov
Keyword(s):  
Dietary Habits ◽  
Food Preferences ◽  
Wide Distribution ◽  
Prey Category ◽  
Active Season ◽  
Non Invasive ◽  
Faecal Samples ◽  
Zootoca Vivipara ◽  
Invasive Method ◽  
Glacial Relict

The Viviparous lizard Zootoca vivipara (Lichtenstein, 1823) is widespread across Eurasia, but in Bulgaria it is considered a glacial relict, restricted to the high mountains. In spite of its wide distribution, there is still lack of knowledge on its dietary habits, especially from the southern part of the range. In order to shed light on its diet, we conducted а study in three mountain sites in Bulgaria (Vitosha, Rila and Stara Planina Mtns.). They were visited in 2016 and 2017 during the lizard's active season (May–September); 343 Viviparous lizards were captured by hand and measured and weighed. To investigate the food preferences, we adopted a non-invasive method, obtaining faecal samples, which were then analysed under a stereoscopic microscope. A total of 290 faecal samples that could be assigned to individual specimens with determined age and sex were collected. We calculated the relative abundance and occurrence of each prey category. Data were compared by sex and age classes (immatures vs. adults) as a whole and for each of the three sites. A total of 19 prey categories were detected and the number of categories per study site varied between 16 and 18. The most common invertebrates were Araneae and Auchenorrhyncha. Statistically significant differences between immatures and adults were found as adults showed more diverse diet. No such differences between sexes were detected.

scholarly journals Stool serology: development of a non-invasive immunological method for the detection of Enterovirus-specific antibodies in Congo gorilla faeces

2020 ◽  
Author(s):  
Youssouf Sereme ◽  
Sandra Madariaga Zarza ◽  
Hacène Medkour ◽  
Inestin Amona ◽  
Florence Fenollar ◽  
...  
Keyword(s):  
Recombinant Virus ◽  
Democratic Republic Of Congo ◽  
Vaccine Antigen ◽  
Paralytic Poliomyelitis ◽  
Specific Antibodies ◽  
Non Invasive ◽  
Polio Vaccine ◽  
Faecal Samples ◽  
Invasive Method ◽  
Humoral Responses

AbstractThe incidence of poliovirus has significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Recently, a new recombinant virus (Enterovirus C/Poliovirus) was identified in humans working as eco-guards and in gorillas in Democratic Republic of Congo, including one gorilla with polio-like sequelae. A strain of this recombinant virus (Ibou002) was also isolated from gorilla faeces. In order to assess the potential role of poliovirus infection, we have developed and optimised a protocol, based on the lyophilisation and solubilisation of small volumes of stool extracts, to detect specific antibodies. First, total immunoglobulins was detected in the concentrated stool extracts. Specific antibodies were then detected in 4/16 gorilla samples and 2/3 human samples by western blot using both the polio vaccine antigen and the Ibou002 antigen and by ELISA using the polio vaccine antigen. Humoral responses were greater with the Ibou002 antigen. We therefore suggest that this recombinant virus could lead to a polio-like disease in the endangered western lowland gorilla. The development of a non-invasive method to detect microorganism-specific immunoglobulins from faecal samples opens up new perspectives for the exploration of humoral responses of pathogens in animals and a greater understanding of zoonotic infectious diseases.

Malfunctional Reorganization in the Developing Limbo-Prefrontal System in Animals: Implications for Human Psychoses?

2001 ◽  
Vol 12 (1) ◽  
pp. 8-14
Author(s):  
Gertraud Teuchert-Noodt ◽  
Ralf R. Dawirs
Keyword(s):  
Prefrontal Cortex ◽  
Mental Disorders ◽  
Dentate Gyrus ◽  
Cognitive Functions ◽  
Experimental Approach ◽  
Regulatory Mechanisms ◽  
Hippocampal Dentate Gyrus ◽  
Non Invasive ◽  
Invasive Method ◽  
Activity Dependent

Abstract: Neuroplasticity research in connection with mental disorders has recently bridged the gap between basic neurobiology and applied neuropsychology. A non-invasive method in the gerbil (Meriones unguiculus) - the restricted versus enriched breading and the systemically applied single methamphetamine dose - offers an experimental approach to investigate psychoses. Acts of intervening affirm an activity dependent malfunctional reorganization in the prefrontal cortex and in the hippocampal dentate gyrus and reveal the dopamine position as being critical for the disruption of interactions between the areas concerned. From the extent of plasticity effects the probability and risk of psycho-cognitive development may be derived. Advance may be expected from insights into regulatory mechanisms of neurogenesis in the hippocampal dentate gyrus which is obviously to meet the necessary requirements to promote psycho-cognitive functions/malfunctions via the limbo-prefrontal circuit.

Bioimpedance Spectroscopy as a Non-invasive Method of Determination of the Hydration Status in Hemodialysis Patients

2010 ◽  
Vol 14 (2) ◽  
pp. 72-77
Author(s):  
J. Rosina ◽  
Jana Vranova ◽  
Ondrej Remes ◽  
Katarina Nehezova ◽  
I. Rychlik
Keyword(s):  
Hemodialysis Patients ◽  
Hydration Status ◽  
Bioimpedance Spectroscopy ◽  
Non Invasive ◽  
Method Of Determination ◽  
Invasive Method

scholarly journals CT perfusion in peripheral arterial disease—hemodynamic differences before and after revascularisation

2021 ◽  
Author(s):  
Patrick Veit-Haibach ◽  
Martin W. Huellner ◽  
Martin Banyai ◽  
Sebastian Mafeld ◽  
Johannes Heverhagen ◽  
...  
Keyword(s):  
Peripheral Arterial Disease ◽  
Ct Perfusion ◽  
Arterial Disease ◽  
Lower Leg ◽  
Therapy Control ◽  
Non Invasive ◽  
Before And After ◽  
Invasive Method ◽  
The Mean ◽  
Peripheral Arterial

Abstract Objectives The purpose of this study was the assessment of volumetric CT perfusion (CTP) of the lower leg musculature in patients with symptomatic peripheral arterial disease (PAD) before and after interventional revascularisation. Methods Twenty-nine consecutive patients with symptomatic PAD of the lower extremities requiring interventional revascularisation were assessed prospectively. All patients underwent a CTP scan of the lower leg, and hemodynamic and angiographic assessment, before and after intervention. Ankle-brachial pressure index (ABI) was determined. CTP parameters were calculated with a perfusion software, acting on a no outflow assumption. A sequential two-compartment model was used. Differences in CTP parameters were assessed with non-parametric tests. Results The cohort consisted of 24 subjects with an occlusion, and five with a high-grade stenosis. The mean blood flow before/after (BFpre and BFpost, respectively) was 7.42 ± 2.66 and 10.95 ± 6.64 ml/100 ml*min−1. The mean blood volume before/after (BVpre and BVpost, respectively) was 0.71 ± 0.35 and 1.25 ± 1.07 ml/100 ml. BFpost and BVpost were significantly higher than BFpre and BVpre in the treated limb (p = 0.003 and 0.02, respectively), but not in the untreated limb (p = 0.641 and 0.719, respectively). Conclusions CTP seems feasible for assessing hemodynamic differences in calf muscles before and after revascularisation in patients with symptomatic PAD. We could show that CTP parameters BF and BV are significantly increased after revascularisation of the symptomatic limb. In the future, this quantitative method might serve as a non-invasive method for surveillance and therapy control of patients with peripheral arterial disease. Key Points • CTP imaging of the lower limb in patients with symptomatic PAD seems feasible for assessing hemodynamic differences before and after revascularisation in PAD patients. • This quantitative method might serve as a non-invasive method, for surveillance and therapy control of patients with PAD.

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