A Fluorometric Method for Measurement of Oxygen Radical-Scavenging Activity of Water-Soluble Antioxidants

2000 ◽  
Vol 284 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Yousry M.A. Naguib
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxygen Radical ◽  
Water Soluble ◽  
Scavenging Activity ◽  
Fluorometric Method

Oxygen radical scavenging activity of curcumin

1990 ◽  
Vol 58 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Elizabeth Kunchandy ◽  
M.N.A. Rao
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxygen Radical ◽  
Scavenging Activity

scholarly journals Changes in Metabolisms of Antioxidant and Cell Wall in Three Pummelo Cultivars during Postharvest Storage

Biomolecules ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 319 ◽  
Author(s):  
Liu ◽  
Liang ◽  
Jiang ◽  
Chen
Keyword(s):  
Cell Wall ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Pectate Lyase ◽  
Water Soluble ◽  
Scavenging Activity ◽  
Total Phenols ◽  
Postharvest Storage ◽  
Total Antioxidant ◽  
Juice Sacs

The juice sacs of pummelo fruit is susceptible to softening during storage at 25 °C, which causes quality deterioration and flavor loss during postharvest pummelo storage. This study investigated the changes in metabolisms of antioxidant and cell wall in juice sacs of three pummelo cultivars—Hongroumiyou (HR), Bairoumiyou (BR) and Huangroumiyou (HuR)—during postharvest storage. The results revealed that, with the extension of storage, the juice sacs of three pummelo cultivars exhibited a decrease in total antioxidant capacity (TAC), DPPH and ABTS radical scavenging activity; a decline in total phenols (TP) content and an increase firstly then a decrease in total ascorbic acid (TAA) content; and a decrease in lipoxygenase (LOX) activity and a rise initially, but a decline in activities of ascorbate peroxidase (APX) and glutathione peroxidase (GPX). Additionally, increased water-soluble pectin (WSP), but declined propectin, ionic-soluble pectin (ISP) and chelator-soluble pectin (CSP); as well as an increase from 0 d to 60 d then followed by a decline in activities of pectinesterase (PE), polygalacturonase (PG) and pectate lyase (PL) were observed. These results suggested that the metabolisms of antioxidant and cell wall could result in softening and senescence of pummelo fruit.

scholarly journals Synthesis of a novel green carbon dots as a bioimaging agent and a drug delivery system for enhanced antioxidant and antibacterial efficacy

2020 ◽  
Author(s):  
Neeraj Tejwan ◽  
Mousumi Kundu ◽  
Anirudh Sharma ◽  
Joydeep Das ◽  
Parames C Sil
Keyword(s):  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Carbon Dots ◽  
Radical Scavenging Activity ◽  
Drug Loading ◽  
Radical Scavenging ◽  
Water Soluble ◽  
Root Extract ◽  
Scavenging Activity

Abstract Background Now-a-days, synthesis of carbon dots (CDs) from renewable sources has attracted considerable attention due to their wide spread availability, lower cost, higher aqueous solubility and more biocompatibility. Here, we have synthesized water soluble CDs from the root extract of red Korean ginseng via simple microwave irradiation. The CDs were further loaded with a natural flavonoid, rutin, to check its efficacy as a drug delivery system. Result The CDs showed a size distribution between 1–4 nm and excitation-dependent fluorescence emission, and could be used as a bio-imaging agent.The CDs showed a drug loading content of 14% and a sustained drug release behavior at physiological conditions. CDs showed excellent free radical scavenging activity both in cell free systems and intra-cellular conditions. Besides, loading with the drug further improved the intra-cellular radical scavenging activity. The CDs and the nanohybrids also showed very little toxicity upto the concentration of 100 µg/mL in normal kidney epithelial cells. The CDs and the nanohybrids also showed a dose-dependent antibacterial effect against both gram negative as well as gram positive bacteria. Conclusion Our synthesized green CDs can be considered as a promising candidate for the biomedical applications due to its facile synthesis, low cytotoxicity, excellent fluorescent properties and potential as drug delivery systems.

scholarly journals Bioactive chemical constituents, acute toxicity and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity of Polyalthia longifolia root

2021 ◽  
Vol 14 (1) ◽  
pp. 018-026
Author(s):  
Charles Akhadelor Unuigbe ◽  
Chinem Fumi Unula ◽  
Anthony Aiwonegbe ◽  
Jeremiah Ogboma Uadia ◽  
Isaac Akhigbe ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Soluble ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Polyalthia Longifolia ◽  
Crude Methanol Extract

Polyalthia longifolia (masquerade tree) is a plant which is believed to possess varied pharmacological and therapeutic values among different populations. The present report investigated the phytochemical composition, proximate, acute toxicity and antioxidant potential of P. longfolia root. All analyses were carried out using established methods; the antioxidant activity of the crude methanol extract and its fractions (n-hexane and ethyl acetate) were examined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay while the total phenolic and flavonoid contents were assessed using the Folin-Ciocalteu and the aluminum chloride calibration methods respectively. The phytochemical analysis revealed the presence of alkaloids, carbohydrate, reducing sugars, tannins, saponins, flavonoids, phenolic compounds and protein in aqueous extract. The proximate analysis showed moisture content, total ash, alcohol extractive value, water extractive value, acid insoluble ash and water soluble ash at 8.80, 9.35, 3.28, 3.29, 2.27 and 7.29% respectively. The ethyl acetate fraction showed the highest antioxidant property compared to the n-hexane fraction and crude methanol extract in all assays conducted. Also, the methanol fraction was found to have the highest flavonoids and phenolic content among the extract and fractions. Oral administration of crude methanol extract of P. longifolia to Swiss mice was relatively non-toxic at a maximum dose of 5000 mg/kg. The root extract and fractions of P. longifolia indicated moderately high level of some phytochemicals with outstanding radical scavenging activity, and therefore substantiate its use as a conventional and comparatively non-toxic plant antioxidant.

scholarly journals Studies on Persicae Semen. III. Oxygen Radical Scavenging Activity of PR-B, an Anti-inflammatory Protein of Persicae Semen

1985 ◽  
Vol 105 (9) ◽  
pp. 895-901 ◽  
Author(s):  
SHIGERU ARICHI ◽  
MICHINORI KUBO ◽  
TADATO TANI ◽  
HIDEO NAKAMURA ◽  
CHIEKO IMAZU ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxygen Radical ◽  
Scavenging Activity ◽  
Inflammatory Protein ◽  
Persicae Semen ◽  
Anti Inflammatory

scholarly journals Characterization of fermented Okara powder and its effect on lipid oxidation of emulsion-type pork sausage during cold storage

2016 ◽  
Author(s):  
Chuanhai Tu ◽  
Shanshan Zhang ◽  
Qiuqin Zhang ◽  
Yu Xiao ◽  
Guangliang Xing ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Dietary Fiber ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Thiobarbituric Acid ◽  
Fiber Content ◽  
Water Soluble ◽  
Good Effect ◽  
Scavenging Activity ◽  
Pork Sausage

In the present study, Okara, a soybean by-product from the production of tofu and soymilk, was processed by solid state fermentation (SSF) with Neurospora crassa Mai 1-1, a filamentous and edible fungus which was firstly isolated in our group. The proximate composition, dietary fiber content, water-soluble polysaccharides and antioxidant activity of Okara was investigated. The crude fat and protein, soluble dietary fiber content and ergosterol content of Okara was significantly increased by SSF with N.crassa . And the antioxidant activity (evaluated by the ABTS radical cation scavenging activity and DPPH radical scavenging activity) of Okara was significantly enhanced by SSF with N. crassa . In addition, emulsion-type sausage was taken as a model system for farther investigation, sausage with fermented Okara power had reduced values of thiobarbituric acid reactive substances (TBARS) ( p < 0.05 ), peroxide value. Therefore, this study demonstrated that fermented Okara showed higher nutrition value and antioxidant activity compared to the non-fermented Okara counterpart, and as an additive has good effect on antioxidant of emulsion-type sausage.

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