Prevalence of H1299R polymorphism in the Factor V gene among the Taif-Saudi Arabia population using polymerase chain reaction-reverse hybridization technique

2011 ◽  
Vol 39 (1) ◽  
pp. 439-443 ◽  
Author(s):  
Nabil Saied Awad ◽  
Adel El-Sayed El-Tarras
Keyword(s):  
Polymerase Chain Reaction ◽  
Saudi Arabia ◽  
Hybridization Technique ◽  
Factor V ◽  
Chain Reaction ◽  
Reverse Hybridization ◽  
Factor V Gene ◽  
Polymerase Chain ◽  
V Gene

scholarly journals Circulation of Dengue Virus Serotypes in the City of Makkah, Saudi Arabia, as Determined by Reverse Transcription Polymerase Chain Reaction

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Sameer R. Organji ◽  
Hussein H. Abulreesh ◽  
Gamal E. H. Osman
Keyword(s):  
Polymerase Chain Reaction ◽  
Saudi Arabia ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Denv Serotypes ◽  
Polymerase Chain ◽  
Low Prevalence ◽  
The City

The present study was aimed to investigate the circulation of four dengue virus (DENV) serotypes in Makkah, Western Saudi Arabia. Blood samples were collected from 25 dengue fever-suspected patients and were subjected to molecular typing for DENV-1, DENV-2, DENV-3, and DENV-4 serotypes of dengue virus, by reverse transcription polymerase chain reaction (RT-PCR), using six sets of primers. Of the 25 samples, only six samples (24%) were found to be positive for dengue virus infection. The prevalence of DENV-1 was higher (50% of DENV-positive samples), as compared to DENV-2 (33.3%) and DENV-3 (16.6%) serotypes. The fourth serotype, DENV-4, was not detected in any of the DENV-positive samples. Although Makkah is considered endemic to dengue fever, we observed low prevalence of dengue virus in the city, which may be attributed to various factors. Nonetheless, the results presented herein confirm the circulation of DENV serotypes in the Western region of Saudi Arabia. To the best of our knowledge, the current study so far is the first report demonstrating the prevalence of the DENV-1 serotype in the city Makkah, Saudi Arabia.

scholarly journals Detection of Campylobacter jejuni and Salmonella typhimurium in chicken using PCR for virulence factor hipO and invA genes (Saudi Arabia)

2021 ◽  
Vol 41 (9) ◽  
Author(s):  
Khaloud M. Alarjani ◽  
Manal F. Elkhadragy ◽  
Abdulrahman H. Al-Masoud ◽  
Hany M. Yehia
Keyword(s):  
Polymerase Chain Reaction ◽  
Saudi Arabia ◽  
16S Rrna ◽  
Campylobacter Jejuni ◽  
Molecular Size ◽  
Strain Atcc ◽  
Widal Test ◽  
Chain Reaction ◽  
Inva Gene ◽  
Polymerase Chain

Abstract Campylobacter jejuni and Salmonella typhimurium are the leading causes of bacterial food contamination in chicken carcasses. Contamination is particularly associated with the slaughtering process. The present study isolated C. jejuni and S. typhimurim from fifty chicken carcass samples, all of which were acquired from different companies in Riyadh, Saudi Arabia. The identification of C. jejuni was performed phenotypically by using a hippurate test and genetically using a polymerase chain reaction with primers for 16S rRNA and hippurate hydrolase (hipO gene). For the dentification of S. typhimurim, a serological Widal test was carried out using serum anti-S. typhimurium antibodies. Strains were genetically detected using invA gene primers. The positive isolates for C. jejuni showed a specific molecular size of 1448 bp for 16S rRNA and 1148 bp for hipO genes. However, the positive isolates of the invA gene exhibited a specific molecular size at 244 bp using polymerase chain reaction (PCR). Comparing sequencing was performed with respect to the invA gene and the BLAST nucleotide isolates that were identified as Salmonella enterica subsp. enterica serovar typhimurium strain ST45, thereby producing a similarity of 100%. The testing identified C.jejuni for hippuricase, GenBank: Z36940.1. While many isolates of Salmonella spp. that contained the invA gene were not necessarily identified as S. typhimurim, the limiting factor for the Widal test used antiS. typhimurum antibodies. The multidrug resistance (MDR) of C. jejuni isolates in chickens was compared with the standard C. jejuni strain ATCC 22931. Similarly, S. typhimurium isolates were compared with the standard S. typhimurium strain ATCC 14028.

Molecular Pathology of Soft Tissue and Bone Tumors

1999 ◽  
Vol 123 (12) ◽  
pp. 1246-1259
Author(s):  
Andrzej Slominski ◽  
Jacobo Wortsman ◽  
Andrew Carlson ◽  
Martin Mihm ◽  
Brian Nickoloff ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Growth Factors ◽  
Soft Tissue ◽  
Reverse Transcription ◽  
Intracellular Signaling ◽  
Bone Tumors ◽  
Cellular Proliferation ◽  
Hybridization Technique ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Objective.—To present recent concepts on the molecular pathogenesis of tumors of soft tissue and bone, and on the use of molecular genetic methods, including their significance as diagnostic markers and prognostic indicators. Data Sources and Study Selection.—Reports on tumors of bone and/or soft tissue published in the English language literature and observations made using specimens available at the Departments of Pathology at Albany Medical College and Loyola University Medical Center. Data Extraction and Synthesis.—Studies on bone and soft tissue tumors containing chromosomal or genetic evaluation were selected for further analysis. Specific chromosomal abnormalities, such as numerical aberrations or translocations with production of fusion genes, were classified according to the tumor of origin. Data were also collected on mutations in tumor suppressor genes, genes coding for growth factors or their receptors, and genes coding for tyrosine kinases. Also noted were mutations of uncertain significance, for which the pathogenic connection between tumor production and mutated gene function is still unclear. Conclusions.—In general, the mutations reported interfere with the action of peptide growth factors coordinating mesenchyme proliferation and differentiation, although membrane-bound receptors expressing the intracellular signaling modifier, tyrosine kinase activity, have also been involved. Functional types of genes most commonly affected include tumor suppressors, oncogenes, and nuclear transcription factors. Thus, the mutations involved in the pathogenesis of soft tissue and bone tumors have affected multiple genes. Moreover, aberrant fusion gene products may be formed in tumoral tissue and may then act as transcription regulators stimulating cellular proliferation. Cytogenetic studies help at the clinical level by demonstrating aneuploidy and increased ploidy, which may correlate with malignant behavior. Diagnostic tumor-specific chromosomal translocations may be detected with Southern hybridization analysis, polymerase chain reaction, reverse-transcription polymerase chain reaction, or with the fluorescence in situ hybridization technique. Notably, early metastatic disease may be detectable in blood specimens using polymerase chain reaction or reverse-transcription polymerase chain reaction techniques.

Enhanced Detection of HIV-1 Sequences Using Polymerase Chain Reaction and a Liquid Hybridization Technique

Vox Sanguinis ◽  
1991 ◽  
Vol 61 (1) ◽  
pp. 24-29 ◽  
Author(s):  
S.M. Bruisten ◽  
M.H.G.M. Koppelmann ◽  
C.L. van der Poel ◽  
J.G. Huisman
Keyword(s):  
Polymerase Chain Reaction ◽  
Hybridization Technique ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Hiv 1
Sign in / Sign up

Export Citation Format

Share Document