Identification and Gene Structure of a Novel Human PLZF-Related Transcription Factor Gene, TZFP

1999 ◽  
Vol 264 (3) ◽  
pp. 789-795 ◽  
Author(s):  
Wen-chang Lin ◽  
Chun-Hung Lai ◽  
Chieh-Ju C. Tang ◽  
Chang-Jen Huang ◽  
Tang K. Tang
Keyword(s):  
Transcription Factor ◽  
Gene Structure ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Related Transcription Factor

scholarly journals Molecular cloning of doublesex and mab-3-related transcription factor 1, forkhead transcription factor gene 2, and two types of cytochrome P450 aromatase in Southern catfish and their possible roles in sex differentiation

2007 ◽  
Vol 194 (1) ◽  
pp. 223-241 ◽  
Author(s):  
Zhihao Liu ◽  
Fengrui Wu ◽  
Baowei Jiao ◽  
Xiuyue Zhang ◽  
Chongjiang Hu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Cytochrome P450 ◽  
Sex Differentiation ◽  
Treated Group ◽  
Transcription Factor Gene ◽  
Forkhead Transcription Factor ◽  
Factor Gene ◽  
Related Transcription Factor ◽  
Transcription Factor 1 ◽  
The Brain

To address the roles of doublesex and mab-3-related transcription factor 1 (Dmrt1), forkhead transcription factor gene 2 (Foxl2), and aromatase in sex differentiation of Southern catfish, the cDNA sequences of these genes were isolated from the gonads. Dmrt1a and Dmrt1b were found to be expressed in the gonads, being higher in the testis. A low expression level of Dmrt1b was also detected in the intestine and kidney of the male. Foxl2 was found to be expressed extensively in the brain (B), pituitary (P), gill and gonads (G), with the highest level in the ovary, indicating the possible involvement of Foxl2 in the B–P–G axis. Cytochrome P450 (Cyp)19b was found to be expressed in the brain, spleen, and gonads, while Cyp19a was only expressed in the gonads and spleen. All-female Southern catfish fry were treated with fadrozole (F), tamoxifen (TAM), and 17β-estradiol (E2) respectively, from 5 to 25 days after hatching (dah). The expression levels of these genes were measured at 65 dah. In the F-, TAM-, and FTAM-treated groups, Dmrt1a and Dmrt1b were up-regulated in the gonad, whereas Foxl2 and Cyp19a were down-regulated, while the expression of Cyp19b in the gonad remained unchanged. Furthermore, down-regulation of Foxl2 and Cyp19b was also detected in the brain. In the E2-treated group, Dmrt1a and Dmrt1b were down-regulated to an undetectable level in the gonad, whereas Foxl2 and Cyp19b were up-regulated in the brain. Consistent with the observed changes in the expressions of these genes, 56, 70, and 80% sex-reversed male individuals were obtained in the F-, TAM-, and F + TAM-treated groups respectively. These results indicate the significant roles of Dmrt1, Foxl2, and Cyp19 in the sex differentiation of Southern catfish.

The HIP116 SNF2/SW12-Related Transcription Factor Gene (SNF2L3) Is Located on Human Chromosome 3q25.1-q26.1

Genomics ◽  
1995 ◽  
Vol 27 (2) ◽  
pp. 381-382 ◽  
Author(s):  
Ying Lin ◽  
Philip L. Sheridan ◽  
Katherine A. Jones ◽  
Glen A. Evans
Keyword(s):  
Transcription Factor ◽  
Human Chromosome ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Related Transcription Factor

scholarly journals Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Desyanti Saulina Sinaga ◽  
Shin-Lon Ho ◽  
Chung-An Lu ◽  
Su-May Yu ◽  
Li-Fen Huang
Keyword(s):  
Transcription Factor ◽  
Recombinant Proteins ◽  
Cultured Cells ◽  
Cell Suspension Cultures ◽  
Genetically Engineered ◽  
Transcription Factor Gene ◽  
Suspension Cells ◽  
Factor Gene ◽  
Related Transcription Factor ◽  
Macrophage Colony

Abstract Background Transgenic plant suspension cells show economic potential for the production of valuable bioproducts. The sugar starvation-inducible rice αAmy3 promoter, together with its signal peptide, is widely applied to produce recombinant proteins in rice suspension cells. The OsMYBS2 transcription factor was shown recently to reduce activation of the αAmy3 promoter by competing for the binding site of the TA box of the αAmy3 promoter with the potent OsMYBS1 activator. In this study, rice suspension cells were genetically engineered to silence OsMYBS2 to enhance the production of recombinant proteins. Results The mouse granulocyte–macrophage colony-stimulating factor (mGM-CSF) gene was controlled by the αAmy3 promoter and expressed in OsMYBS2-silenced transgenic rice suspension cells. Transcript levels of the endogenous αAmy3 and the transgene mGM-CSF were increased in the OsMYBS2-silenced suspension cells. The highest yield of recombinant mGM-CSF protein attained in the OsMYBS2-silenced transgenic suspension cells was 69.8 µg/mL, which is 2.5-fold that of non-silenced control cells. The yield of recombinant mGM-CSF was further increased to 118.8 µg/mL in cultured cells derived from homozygous F5 seeds, which was 5.1 times higher than that of the control suspension cell line. Conclusions Our results demonstrate that knockdown of the transcription factor gene OsMYBS2 increased the activity of the αAmy3 promoter and improved the yield of recombinant proteins secreted in rice cell suspension cultures.

scholarly journals Global Analysis of WOX Transcription Factor Gene Family in Brassica napus Reveals Their Stress- and Hormone-Responsive Patterns

2018 ◽  
Vol 19 (11) ◽  
pp. 3470 ◽  
Author(s):  
Mang-Mang Wang ◽  
Ming-Ming Liu ◽  
Feng Ran ◽  
Peng-Cheng Guo ◽  
Yun-Zhuo Ke ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Brassica Napus ◽  
Gene Family ◽  
Gene Structure ◽  
Stress Responses ◽  
Expression Profiles ◽  
Global Analysis ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Transcription Factor Gene Family

The plant-specific WUSCHEL-related homeobox (WOX) transcription factor gene family is important for plant growth and development but little studied in oil crops. We identified and characterized 58 putative WOX genes in Brassica napus (BnWOXs), which were divided into three major clades and nine subclades based on the gene structure and conserved motifs. Collinearity analysis revealed that most BnWOXs were the products of allopolyploidization and segmental duplication events. Gene structure analysis indicated that introns/exons and protein motifs were conserved in each subclade and RNA sequencing revealed that BnWOXs had narrow expression profiles in major tissues and/or organs across different developmental stages. The expression pattern of each clade was highly conserved and similar to that of the sister and orthologous pairs from Brassica rapa and Brassica oleracea. Quantitative real-time polymerase chain reaction showed that members of the WOX4 subclade were induced in seedling roots by abiotic and hormone stresses, indicating their contribution to root development and abiotic stress responses. 463 proteins were predicted to interact with BnWOXs, including peptides regulating stem cell homeostasis in meristems. This study provides insights into the evolution and expression of the WOX gene family in B. napus and will be useful in future gene function research.

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