Protein Delivery by Pseudomonas Type III Secretion System: Ex Vivo Complementation of p67phox-Deficient Chronic Granulomatous Disease

ABSTRACT Twelve Pseudomonas aeruginosa cystic fibrosis isolates that are not able to exert a type III secretion system (TTSS)-dependent cytotoxicity towards phagocytes have been further studied. The strains, although possessing TTSS genes and exsA, which encodes a positive regulator of the TTSS regulon, showed no transcriptional activation of the exsCBA regulatory operon. The expression of exsA in trans restored the in vitro secretion of TTSS proteins and ex vivo cytotoxicity.

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Bacterial type III secretion system as a protein delivery tool for a broad range of biomedical applications

Biotechnology Advances ◽

10.1016/j.biotechadv.2018.01.016 ◽

2018 ◽

Vol 36 (2) ◽

pp. 482-493 ◽

Cited By ~ 13

Author(s):

Fang Bai ◽

Zhenpeng Li ◽

Akihiro Umezawa ◽

Naohiro Terada ◽

Shouguang Jin

Keyword(s):

Type Iii Secretion ◽

Protein Delivery ◽

Biomedical Applications ◽

Type Iii Secretion System ◽

Secretion System ◽

Type Iii ◽

Bacterial Type

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Delivery of Heterologous Proteins, Enzymes, and Antigens via the Bacterial Type III Secretion System

Microorganisms ◽

10.3390/microorganisms8050777 ◽

2020 ◽

Vol 8 (5) ◽

pp. 777 ◽

Cited By ~ 1

Author(s):

Heather A. Pendergrass ◽

Aaron E. May

Keyword(s):

Type Iii Secretion ◽

Protein Delivery ◽

Type Iii Secretion System ◽

Secretion System ◽

Host Cells ◽

Heterologous Proteins ◽

Communication Tool ◽

Type Iii ◽

Heterologous Protein Secretion ◽

Multimeric Protein

The Type III Secretion System (T3SS) is a multimeric protein complex composed of over 20 different proteins, utilized by Gram-negative bacteria to infect eukaryotic host cells. The T3SS has been implicated as a virulence factor by which pathogens cause infection and has recently been characterized as a communication tool between bacteria and plant cells in the rhizosphere. The T3SS has been repurposed to be used as a tool for the delivery of non-native or heterologous proteins to eukaryotic cells or the extracellular space for a variety of purposes, including drug discovery and drug delivery. This review covers the methodology of heterologous protein secretion as well as multiple cases of utilizing the T3SS to deliver heterologous proteins or artificial materials. The research covered in this review will serve to outline the scope and limitations of utilizing the T3SS as a tool for protein delivery.

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The Animal Pathogen-Like Type III Secretion System Is Required for the Intracellular Survival of Burkholderia mallei within J774.2 Macrophages

Infection and Immunity ◽

10.1128/iai.01939-05 ◽

2006 ◽

Vol 74 (7) ◽

pp. 4349-4353 ◽

Cited By ~ 23

Author(s):

Wilson J. Ribot ◽

Ricky L. Ulrich

Keyword(s):

Type Iii Secretion ◽

Protein Delivery ◽

Actin Polymerization ◽

Type Iii Secretion System ◽

Secretion System ◽

Burkholderia Mallei ◽

Intracellular Growth ◽

Type Iii ◽

Membrane Protrusions ◽

Cell Spread

ABSTRACT Burkholderia mallei is a highly infectious gram-negative pathogen and is the causative agent of human and animal glanders. By generating polar mutations (disruption of bsaQ and bsaZ) in the B. mallei ATCC 23344 animal pathogen-like type III secretion system (TTS), we demonstrate that this bacterial protein delivery system is required for intracellular growth of B. mallei in J774.2 cells, formation of macrophage membrane protrusions, actin polymerization, and phagosomal escape. These findings suggest that TTS plays a role in the intracellular trafficking of B. mallei and may facilitate cell-to-cell spread via actin-based motility.

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