The Receptor-Mediated Uptake, Survival, Replication, and Drug Sensitivity of Mycobacterium tuberculosis within the Macrophage-like Cell Line THP-1: A Comparison with Human Monocyte-Derived Macrophages

1999 ◽  
Vol 197 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Richard W. Stokes ◽  
Daniel Doxsee
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Cell Line ◽  
Drug Sensitivity ◽  
Human Monocyte

A requirement for cholesterol for phorbol ester-induced adhesion of a human monocyte-like cell line

1987 ◽  
Vol 144 (3) ◽  
pp. 1167-1174 ◽  
Author(s):  
M. Esfahani ◽  
S. Hathotuwegama ◽  
A. Kalenak ◽  
L. Scerbo ◽  
H.M. Brown
Keyword(s):  
Cell Line ◽  
Phorbol Ester ◽  
Human Monocyte

scholarly journals Varicella-Zoster Virus Activates Inflammatory Cytokines in Human Monocytes and Macrophages via Toll-Like Receptor 2

2005 ◽  
Vol 79 (20) ◽  
pp. 12658-12666 ◽  
Author(s):  
Jennifer P. Wang ◽  
Evelyn A. Kurt-Jones ◽  
Ok S. Shin ◽  
Michael D. Manchak ◽  
Myron J. Levin ◽  
...  
Keyword(s):  
Varicella Zoster Virus ◽  
Cell Line ◽  
Human Monocyte ◽  
Human Cell Line ◽  
Mouse Cell ◽  
Human Monocytes ◽  
Toll Like Receptor ◽  
Herpes Simplex Viruses ◽  
Varicella Zoster ◽  
Toll Like Receptor 2

ABSTRACT The pattern recognition receptor Toll-like receptor 2 (TLR2) has been implicated in the response to several human viruses, including herpes simplex viruses (types 1 and 2) and cytomegalovirus. We demonstrated that varicella-zoster virus (VZV) activates inflammatory cytokine responses via TLR2. VZV specifically induced interleukin-6 (IL-6) in human monocytes via TLR2-dependent activation of NF-κB, and small interfering RNA designed to suppress TLR2 mRNA reduced the IL-6 response to VZV in human monocyte-derived macrophages. Unlike other herpesviruses, the cytokine response to VZV was species specific. VZV did not induce cytokines in murine embryonic fibroblasts or in a mouse cell line, although VZV did activate NF-κB in a human cell line expressing a murine TLR2 construct. Together, these results suggest that TLR2 may play a role in the inflammatory response to VZV infection.

scholarly journals Matching anticancer compounds and tumor cell lines by neural networks with ranking loss

2022 ◽  
Vol 4 (1) ◽  
Author(s):  
Paul Prasse ◽  
Pascal Iversen ◽  
Matthias Lienhard ◽  
Kristina Thedinga ◽  
Chris Bauer ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Cancer Cell ◽  
Drug Sensitivity ◽  
Inhibitory Concentration ◽  
Cancer Cell Line ◽  
Tumor Cell Line ◽  
Ranking Problem ◽  
Ranking Loss

ABSTRACT Computational drug sensitivity models have the potential to improve therapeutic outcomes by identifying targeted drug components that are likely to achieve the highest efficacy for a cancer cell line at hand at a therapeutic dose. State of the art drug sensitivity models use regression techniques to predict the inhibitory concentration of a drug for a tumor cell line. This regression objective is not directly aligned with either of these principal goals of drug sensitivity models: We argue that drug sensitivity modeling should be seen as a ranking problem with an optimization criterion that quantifies a drug’s inhibitory capacity for the cancer cell line at hand relative to its toxicity for healthy cells. We derive an extension to the well-established drug sensitivity regression model PaccMann that employs a ranking loss and focuses on the ratio of inhibitory concentration and therapeutic dosage range. We find that the ranking extension significantly enhances the model’s capability to identify the most effective anticancer drugs for unseen tumor cell profiles based in on in-vitro data.

scholarly journals Tuberculous Meningitis: The Microbiological Laboratory Diagnosis and Its Drug Sensitivity Patterns

2019 ◽  
Vol 3 (2) ◽  
pp. 35
Author(s):  
Titiek Sulistyowati ◽  
Deby Kusumaningrum ◽  
Eko Budi Koendhori ◽  
Ni Made Mertaniasih
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Tuberculous Meningitis ◽  
Drug Sensitivity ◽  
Mycobacterium Tuberculosis Complex ◽  
Laboratory Diagnosis ◽  
Clinical Microbiology Laboratory ◽  
Tuberculosis Complex ◽  
First Line ◽  
Sensitivity Pattern ◽  
Microbiological Laboratory

Background: Tuberculosis continues one of the major challenges to global health. Mycobacterium tuberculosis complex can affect any organ other than the lung parenchyma, include central nervous system. The mortality rate of tuberculous meningitis (TBM) are high worldwide with up to half of survivors suffering irreversible sequelae. Diagnosis of TBM is difficult due to paucibacillary, various clinical manifestation, and invasive procedure to appropriate specimens. Objective: The objectiveis to study the positivity rate of microbiological laboratory diagnosis and its drug sensitivity patterns of TBM patients in Dr. Soetomo Hospital Surabaya during October 2015 until September 2016. Methods: Specimens were cerebrospinal fluids. Identification and drug anti TB sensitivity test were done by BACTEC MGIT 960 system in Clinical Microbiology Laboratory Dr. Soetomo Hospital Surabaya. Result: Most patients with TBM were women (54.29%). Based on age groups, most dominant was adult population (65.71%). Proportion percentage of positive M. tuberculosis complex among 180 specimens were 19.44%. First line anti TB drug sensitivity pattern of 35 isolates were 1 monoresistant, 1 poly-resistant, no multiple drug resistant (MDR), and 33 pan-susceptible. Conclusion: Positivity rate of Mycobacterium tuberculosis complex laboratory diagnosis from TBM suspect patients were low. There was no MDR TB in this study, but mono-resistant and poly-resistant. Microbiological diagnosis was important to give information of active disease and drug sensitivity pattern. Resistance to first line anti TB drugs is alarming to properly manage TBM patients.

Human monocyte cell line (U937) releases suppressive IgG-binding factor(s)

1986 ◽  
Vol 16 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Charles Félix Calvo ◽  
Shinichiro Watanabe ◽  
Didier Mètivier ◽  
Anna Senik
Keyword(s):  
Cell Line ◽  
Human Monocyte ◽  
Igg Binding ◽  
Binding Factor ◽  
Human Monocyte Cell Line ◽  
Monocyte Cell

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

10.3791/62652 ◽  
2021 ◽  
Author(s):  
Katharina M. Scheurlen ◽  
Dylan L. Snook ◽  
Sarah A. Gardner ◽  
Maurice R. Eichenberger ◽  
Susan Galandiuk
Keyword(s):  
Cell Line ◽  
Leukemia Cell ◽  
Human Monocyte ◽  
Leukemia Cell Line ◽  
Macrophage Differentiation

Spoligotyping and Drug Sensitivity of Mycobacterium tuberculosis isolated from Pulmonary Tuberculosis Patients in the Arsi Zone of South Eastern Ethiopia

2019 ◽  
Author(s):  
Belete Haile Nega ◽  
Ketema Tafess ◽  
Aboma Zewude ◽  
Bazezew Yenew ◽  
Gilman SIU ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Drug Sensitivity ◽  
Scientific Information ◽  
Rpob Gene ◽  
Drug Susceptibility ◽  
Strain Identification ◽  
First Line ◽  
Eastern Ethiopia ◽  
Arsi Zone ◽  
Mycobacterium Africanum

Abstract Background Tuberculosis (TB) is one of the leading disease causing morbidity and mortality in different zones of Ethiopia including the Arsi Zone. However, little or no scientific information is available on the strains of Mycobacterium tuberculosis and their drug sensitivity profiles in this Zone. This study was conducted to identify the strains of M. tuberculosis and evaluate their drug sensitivity profiles. Methodology A total of 111 clinical isolates of M. tuberculosis from patients with pulmonary TB in the Arsi Zone were used for this study. The region of difference 9 (RD 9)-based polymerase chain reaction (PCR)and spoligotyping methods were used for speciation and strain identification of Mycobacterium tuberculosis respectively.The spoligotyping patterns were compared with the international SpolDB4 (SITVIT) and Run TB-Lineage used for the identification of lineages. The phenotypic drug susceptibility patterns were confirmed by BD BactecMGIT 960 SIRE test and GenoType MTBDRplus line probe assays were used for the detection the drug resistance-conferring mutations of the isolates. Result The spoligotype patterns of 83% (92/111) of the isolates were interpretable and 56 different patterns were identified. Twenty-two of these patterns were shared types while the remaining 34 were orphans. The predominant shared types were spoligotype international type (SIT) 149 and SIT53, each consisting of 12 and 11 isolates, respectively. The lineages identified were Euro-American, East-African-Indian, Mycobacterium-africanum, and Indo-Oceanic in descending order. Phenotypically, 17.2% of the 64 tested isolates were resistant to any of the four first-line drugs while 3.1% of them were multi-drug resistant (MDR). Higher (6.2%) monoresistance was observed to Streptomycin followed by Isoniazid (3.1%) while no resistance was observed either to Rifampicin or to Ethambutol. Genotypically, five (5.4%) isolates were resistant to Isoniazid and mutated at codon S315T1 of katG. On the other hand, only 1.1% of the isolates was resistant to Rifampicin and mutated at codon S531L of rpoB gene. Conclusion The proportion of orphan strains isolated in this study was high, which could suggest the presence of new strains in the Zone. Moreover, the study showed relatively high percentage of mono-resistance to any four first-line drugs warranting for the need to strengthen the control efforts.

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