Heat Shock Response and Thermal Resistance in Cultured Human Retinal Pigment Epithelium

1993 ◽  
Vol 56 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Masato Wakakura ◽  
Wallace S. Foulds
Keyword(s):  
Retinal Pigment Epithelium ◽  
Heat Shock ◽  
Thermal Resistance ◽  
Heat Shock Response ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium ◽  
Shock Response

scholarly journals Induction of Heat Shock Protein 70 in Mouse RPE as an In Vivo Model of Transpupillary Thermal Stimulation

2020 ◽  
Vol 21 (6) ◽  
pp. 2063
Author(s):  
Mooud Amirkavei ◽  
Marja Pitkänen ◽  
Ossi Kaikkonen ◽  
Kai Kaarniranta ◽  
Helder André ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Response ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
In Vivo Model ◽  
Tunel Staining ◽  
Long Duration ◽  
Shock Response

The induction of heat shock response in the macula has been proposed as a useful therapeutic strategy for retinal neurodegenerative diseases by promoting proteostasis and enhancing protective chaperone mechanisms. We applied transpupillary 1064 nm long-duration laser heating to the mouse (C57Bl/6J) fundus to examine the heat shock response in vivo. The intensity and spatial distribution of heat shock protein (HSP) 70 expression along with the concomitant probability for damage were measured 24 h after laser irradiation in the mouse retinal pigment epithelium (RPE) as a function of laser power. Our results show that the range of heating powers for producing heat shock response while avoiding damage in the mouse RPE is narrow. At powers of 64 and 70 mW, HSP70 immunostaining indicates 90 and 100% probability for clearly elevated HSP expression while the corresponding probability for damage is 20 and 33%, respectively. Tunel staining identified the apoptotic regions, and the estimated 50% damaging threshold probability for the heating (ED50) was ~72 mW. The staining with Bestrophin1 (BEST1) demonstrated RPE cell atrophy with the most intense powers. Consequently, fundus heating with a long-duration laser provides an approachable method to develop heat shock-based therapies for the RPE of retinal disease model mice.

scholarly journals MCP-1–Activated Monocytes Induce Apoptosis in Human Retinal Pigment Epithelium

2011 ◽  
Vol 52 (8) ◽  
pp. 6026 ◽  
Author(s):  
Dongli Yang ◽  
Susan G. Elner ◽  
Xun Chen ◽  
Matthew G. Field ◽  
Howard R. Petty ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium ◽  
Activated Monocytes

scholarly journals Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium

2015 ◽  
Vol 14 (11) ◽  
pp. 1983-1990 ◽  
Author(s):  
Leopold Adler ◽  
Nicholas P. Boyer ◽  
David M. Anderson ◽  
Jeffrey M. Spraggins ◽  
Kevin L. Schey ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Central Area ◽  
Human Retinal Pigment Epithelium ◽  
Peripheral Areas

The levels of the bis-retinoid A2E cannot account for the high levels of lipofuscin fluorescence found in the central area of the human retinal pigment epithelium.

Production of interleukin-6 by human retinal pigment epithelium in vitro and its regulation by other cytokines

1992 ◽  
Vol 11 (sup1) ◽  
pp. 173-179 ◽  
Author(s):  
Mark T. Benson ◽  
Lynda Shepherd ◽  
Robert C. Rees ◽  
Ian G. Rennie
Keyword(s):  
Retinal Pigment Epithelium ◽  
Interleukin 6 ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium

Opticin production is reduced by hypoxia and VEGF in human retinal pigment epithelium via MMP-2 activation

Cytokine ◽  
2012 ◽  
Vol 59 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Jin Ma ◽  
Tie Pei Zhu ◽  
Morten C. Moe ◽  
Panpan Ye ◽  
Ke Yao
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium

scholarly journals CNTF Mediates Neurotrophic Factor Secretion and Fluid Absorption in Human Retinal Pigment Epithelium

PLoS ONE ◽  
2011 ◽  
Vol 6 (9) ◽  
pp. e23148 ◽  
Author(s):  
Rong Li ◽  
Rong Wen ◽  
Tina Banzon ◽  
Arvydas Maminishkis ◽  
Sheldon S. Miller
Keyword(s):  
Retinal Pigment Epithelium ◽  
Neurotrophic Factor ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Fluid Absorption ◽  
Human Retinal Pigment Epithelium ◽  
Factor Secretion
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