Activity of trisodium phosphate compared with sodium hydroxide wash solutions against Listeria monocytogenes attached to chicken skin during refrigerated storage

2002 ◽  
Vol 19 (1) ◽  
pp. 57-63 ◽  
Author(s):  
R Capita ◽  
C Alonso-Calleja ◽  
M del Camino Garcı́a-Fernández ◽  
B Moreno
Keyword(s):  
Listeria Monocytogenes ◽  
Sodium Hydroxide ◽  
Trisodium Phosphate ◽  
Refrigerated Storage ◽  
Chicken Skin

Efficacy of Trisodium Phosphate Solutions in Reducing Listeria monocytogenes Populations on Chicken Skin during Refrigerated Storage

2001 ◽  
Vol 64 (10) ◽  
pp. 1627-1630 ◽  
Author(s):  
ROSA CAPITA ◽  
CARLOS ALONSO-CALLEJA ◽  
CAMINO GARCÍA-FERNÁNDEZ ◽  
BENITO MORENO
Keyword(s):  
Listeria Monocytogenes ◽  
Trisodium Phosphate ◽  
Refrigerated Storage ◽  
Sterile Water ◽  
Water Control ◽  
Microbial Monitoring ◽  
Chicken Skin ◽  
Phosphate Solutions ◽  
Sampling Times ◽  
Skin Samples

Chicken skin inoculated with 108 CFU/ml of Listeria monocytogenes was dipped for 15 min in sterile water (control) and in 8, 10, or 12% trisodium phosphate (TSP) solutions. Skin samples were stored at 2°C for 5 days, with microbial monitoring on days 0, 1, 3, and 5 after treatment. Compared to the water dip, all TSP treatments significantly (P < 0.05) reduced L. monocytogenes populations on chicken skin. The concentration of the TSP was a significant factor in reducing the populations of the bacteria at days 0, 1, 3, and 5 of refrigerated storage. For all sampling times, the best outcomes were attained with the highest TSP concentration studied (12%). Bacterial reductions in counts during the first day of storage were between 1.52 and 2.70 log10 cycles for 8 and 12% TSP-treated samples, respectively. Significantly greater reductions were observed from the third day of refrigerated storage onward. This occurred largely because populations of L. monocytogenes on control samples increased somewhat, but on TSP-treated samples the pathogen remained practically constant. Differences between L. monocytogenes counts in skin samples immersed in water and those treated with TSP ranged from 2.10 (8% TSP-treated samples) and 3.63 (12% TSP-treated samples) log10 cycles on day 5 of storage. These results indicated that TSP is effective against L. monocytogenes in chicken meat, especially after several days of refrigerated storage.

Effectiveness of Trisodium Phosphate against Listeria monocytogenes on Excised and Nonexcised Chicken Skin

2003 ◽  
Vol 66 (1) ◽  
pp. 61-64 ◽  
Author(s):  
ROSA CAPITA ◽  
CARLOS ALONSO-CALLEJA ◽  
MIGUEL PRIETO ◽  
MARÍA del CAMINO GARCÍA-FERNÁNDEZ ◽  
BENITO MORENO
Keyword(s):  
Listeria Monocytogenes ◽  
Tap Water ◽  
Trisodium Phosphate ◽  
Sample Type ◽  
Refrigerated Storage ◽  
Water Control ◽  
Chicken Skin ◽  
Storage Times ◽  
And Storage ◽  
Skin Samples

The influence of sample type (i.e., excised versus nonexcised chicken skin) on the efficiency of trisodium phosphate (TSP) solutions in reducing Listeria monocytogenes populations and inhibiting their growth during refrigerated storage was studied. Whole chicken legs and excised chicken leg skin fragments inoculated with 108 CFU of L. monocytogenes per ml were dipped for 15 min in sterile tap water (control) or in a solution containing 8, 10, or 12% TSP. L. monocytogenes counts were determined after 0, 1, 3, and 5 days of refrigerated storage (2°C). The decontamination effect of TSP was greater for excised skin than for whole legs. Microbial differences between control and TSP-treated samples were significantly larger for excised skin than for whole legs for 9 (75%) of 12 tested combinations of TSP concentrations and storage times. These differences varied from 1.05 ± 0.26 log10 cycles (day 1) to 3.30 ± 0.14 log10 cycles (day 5) for nonexcised-skin samples (whole legs) and from 1.54 ± 0.48 log10 cycles (day 1) to 4.28 ± 0.86 log10 cycles (day 5) for excised-skin samples. Significantly larger reductions were observed from the third day of refrigerated storage onward. The TSP concentration was a significant factor in the reduction of L. monocytogenes populations. These results suggest that bacteria are more readily accessible to TSP in excised than in nonexcised chicken skin and that the type of sample used to ascertain the efficacy of antimicrobial surface treatments may influence the findings of this type of study.

Note. Effect of trisodium phosphate on mesophilic and psychrotrophic bacterial flora attached to the skin of chicken carcasses during refrigerated storage Nota. Efecto del fosfato trisódico en los microorganismos mesófilos y psicrotrofos presentes en la piel de canales de pollo durante su almacenamiento en refrigeración

2000 ◽  
Vol 6 (4) ◽  
pp. 345-350 ◽  
Author(s):  
R. Capita ◽  
C. Alonso-Calleja ◽  
M.T. García Arias ◽  
B. Moreno ◽  
M.C. García-Fernández
Keyword(s):  
Tap Water ◽  
Bacterial Flora ◽  
Trisodium Phosphate ◽  
Refrigerated Storage ◽  
Water Control ◽  
Bacterial Populations ◽  
Ph Values ◽  
Popula Tions ◽  
Chicken Skin ◽  
Plate Counts

The potential for using trisodium phosphate (TSP) to reduce mesophilic and psychrotrophic popula tions on the skin of chicken carcasses was explored. Skin samples were immersed in sterile tap water (control) or an 8%, 10% or 12% solution of TSP at 20 °C for 15 min. Surface pH values and mesophilic and psychrotrophic plate counts were determined after 0, 1, 3 and 5 days of storage at 2° C. After washing, bacterial populations were significantly smaller in the samples treated with TSP than in the controls. The concentration of the TSP solution was a significant factor in reducing the populations of the bacteria on chicken skin. Before storage, the reduction in the presence of bacteria achieved in treated samples with respect to controls ranged between 0.95 log10 cycles and 1.78 log10 cycles in the case of mesophilic microorganisms, and 0.92 log10 cycles and 1.94 log10 cycles in the case of psychrotrophic strains. These differences between the concentrations of bacteria in samples immersed in water and those treated with TSP increased over time, ranging from 2.35 log 10 cycles to 3.08 log10 cycles (mesophilic microorganisms), and from 2.79 log10 cycles to 4.09 log10 cycles (psychrotrophic microorganisms) on day 5 of storage. The pH of the skin remained more or less constant throughout the study period, ranging between 8 and 9 in skin treated with TSP, depending on the concentration, while it was two units lower in the control samples.

Trisodium phosphate enhanced phage lysis of Listeria monocytogenes growth on fresh-cut produce

LWT ◽  
2017 ◽  
Vol 86 ◽  
pp. 312-317 ◽  
Author(s):  
Adebola O. Oladunjoye ◽  
S. Singh ◽  
Oluwatosin A. Ijabadeniyi
Keyword(s):  
Listeria Monocytogenes ◽  
Trisodium Phosphate ◽  
Fresh Cut

scholarly journals A comparison of dynamic tertiary and competition models for describing the fate of Listeria monocytogenes in Minas fresh cheese during refrigerated storage

2019 ◽  
Vol 79 ◽  
pp. 48-60 ◽  
Author(s):  
Vasco A.P. Cadavez ◽  
Fernanda B. Campagnollo ◽  
Rosicléia A. Silva ◽  
Clara M. Duffner ◽  
Donald W. Schaffner ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Refrigerated Storage ◽  
Competition Models ◽  
Fresh Cheese

scholarly journals Listeria monocytogenes Control using Clean-Label Ingredients

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
E. Heintz ◽  
K. Glass ◽  
J. Lim
Keyword(s):  
Listeria Monocytogenes ◽  
Meat Products ◽  
Culture Collection ◽  
Refrigerated Storage ◽  
Food Research Institute ◽  
Food Research ◽  
Cooked Meat ◽  
Inhibitive Action ◽  
One Way Anova

ObjectivesWorld’s largest outbreak of listeriosis in South Africa last year, remind us that Listeria monocytogenes contamination and growth is still of major concern in refrigerated RTE meats. The same time customers demand for clean label food safety solutions. Provian NDV, a fermented vinegar based powder, was developed to provide a clean label solution that inhibits Listeria monocytogenes during long term refrigerated storage. This document describes the effect of chemical derived acetates and Provian NDV, a novel vinegar based product, on the inhibition of Listeria monocytogenes in a cooked meat applicationMaterials and MethodsFive treatments of cured deli-style ham were tested. The pork ham contained 72–74% (w/w) moisture, 1.75 ± 0.1% (w/w) salt, and pH 6.2–6.4, 156 mg/kg sodium nitrite and 547 mg/kg sodium erythorbate. The treatments included a control without antimicrobials and different concentrations of a chemically derived acetates (0.5% and 0.75%) and Provian® NDV (0.5%, 0.65%). Cooked products were surface-inoculated with 3-log10 CFU/g of a cocktail of 5 strains of Listeria monocytogenes from the culture collection of Food research institute, Wisconsin University including serotypes 4b, 1/2a, and 1/2b. All strains were isolated from RTE- cooked meat products. Inoculated slices (100 g/package) were vacuum-packaged and stored at 4°C and 7°C for 8 to 12 wk. Per treatment triplicate samples were assayed by enumerating on modified Oxford Agar. One way ANOVA was used to analyze significance, p < 0.05. Except from the triplicate repeat, this study was conducted twice independently (trial 1, 5 treatments in triplicate and trial 2 including same treatments, also in triplicate.)ResultsControl Ham supported > 1 log increase of L. monocytogenes at 4- and 2-weeks storage at 4 and 7°C, respectively. In contrast, hams supplemented with 0.5 or 0.75% chemical acetates or 0.65% Provian® NDV inhibited the Listeria growth for 12 and 8 wk at 4 and 7°C, respectively. Inhibition of Listeria on ham supplemented with 0.5% Provian®NDV was further affected by pH and moisture. Ham supplemented with 0.5% Provian® NDV in the trial 1 (71.5% moisture, pH 6.2) delayed Listeria for 12 wk storage at 4°C, whereas individual samples of trial 1 (72.9% moisture, pH 6.3) supported growth (> 1 log increase) at 8 wk. Similar trends were observed at 7°C. The images below reflect the results of trial 1 only.ConclusionThis study confirms the efficacy of acetates on the inhibition of Listeria monocytogenes. Next, this study shows that a product based on natural fermented vinegar, Provian NDV, has a comparable growth inhibitive action in a cured ready-to eat ham. This illustrates that most relevant serotypes (4b, 1/2b and 1/2a) of Listeria moncytogenes can be controlled using an ingredient based on natural fermented vinegar.Figure 4.

scholarly journals Direct Comparison of the N-Acetyl-L-Cysteine-Sodium Hydroxide and the Trisodium Phosphate Digestion Methods for the Culture of Mycobacteria

1968 ◽  
Vol 16 (3) ◽  
pp. 506-508 ◽  
Author(s):  
Margaret E. Martin ◽  
Charles L. Horton ◽  
A. Leonard Sheffner ◽  
James D. Solomon
Keyword(s):  
Sodium Hydroxide ◽  
Trisodium Phosphate ◽  
Digestion Methods

Evaluation of Nisin-Coated Cellulose Casings for the Control of Listeria monocytogenes Inoculated onto the Surface of Commercially Prepared Frankfurters†‡

2004 ◽  
Vol 67 (5) ◽  
pp. 1017-1021 ◽  
Author(s):  
JOHN B. LUCHANSKY ◽  
JEFFREY E. CALL
Keyword(s):  
Listeria Monocytogenes ◽  
Surface Area ◽  
Internal Surface ◽  
Refrigerated Storage ◽  
Internal Surface Area ◽  
Appreciable Increase ◽  
Potassium Lactate ◽  
Selective Agar ◽  
Peptone Water ◽  
Sodium Diacetate

Commercially prepared frankfurters were formulated with and without ~1.4% potassium lactate and 0.1% sodium diacetate and were subsequently processed in cellulose casings coated with and without nisin (~50,000 IU per square inch of internal surface area) to control the outgrowth of Listeria monocytogenes during refrigerated storage. The frankfurters were inoculated with ~5 log CFU per package of a five-strain mixture of L. monocytogenes and then vacuum sealed before being stored at 4° C for 60 to 90 days. Surviving organisms were recovered and enumerated by rinsing each package with 18 ml of sterile 0.1% peptone water and plating onto MOX selective agar. The data for each of two trials were averaged. In packages that contained frankfurters formulated with potassium lactate and sodium diacetate and prepared in nisin-coated casings, L. monocytogenes levels decreased by 1.15 log CFU per package after 90 days of storage. L. monocytogenes levels decreased by 0.95 log CFU per package in frankfurters that were prepared in casings that were not coated with nisin. In packages of frankfurters that were formulated without potassium lactate and sodium diacetate and prepared in nisin-coated casings, L. monocytogenes levels decreased by 0.88 log CFU per package after 15 days of storage but then increased appreciablythereafter over a 60-day period of refrigerated storage. There was also an appreciable increase in pathogen numbers during 60 days of storage in otherwise similar frankfurters formulated without potassium lactate and sodium diacetate prepared in casings that were not coated with nisin. These data confirm that potassium lactate and sodium diacetate display listeriostatic activity as an ingredient of commercial frankfurters. These data also establish that cellulose casings coated with nisin display only moderate antilisterial activity in vacuum-sealed packages of commercially prepared frankfurters during storage at 4° C.

Thermal Inactivation of Listeria monocytogenes in Chicken Gravy

1992 ◽  
Vol 55 (7) ◽  
pp. 492-496 ◽  
Author(s):  
I-PING D. HUANG ◽  
AHMED E. YOUSEF ◽  
ELMER H. MARTH ◽  
M. EILEEN MATTHEWS
Keyword(s):  
Heat Treatment ◽  
Listeria Monocytogenes ◽  
Heat Resistance ◽  
Thermal Inactivation ◽  
Refrigerated Storage ◽  
Department Of Agriculture ◽  
Large Numbers ◽  
Z Value ◽  
D Values ◽  
The U.S

Heat resistance of Listeria monocytogenes strains V7 and Scott A in chicken gravy and changes in heat resistance during refrigerated storage were studied. After chicken gravy was made, it was cooled to 40°C, inoculated with 105 CFU L. monocytogenes per ml of gravy, and then stored at 7°C for 10 d. Gravy was heated at 50, 55, 60, and 65°C immediately after inoculation and after 1, 3, 5, and 10 d of refrigerated storage. The D values for strains Scott A and V7 in gravy heated at 50°C at day 0 were 119 and 195 min and at day 10 they were 115 and 119 min, respectively, whereas at 65°C comparable values at day 0 were 0.48 and 0.19 min and at day 10 they were 0.014 and 0.007 min. Heat resistance (expressed as D values) was greater at day 0 than at the end of refrigerated storage. The z values ranged from 3.41 to 6.10°C and were highest at the early stages of chill storage and then decreased at the later stages. Strain V7 was more heat resistant than Scott A at 50°C. Strain Scott A always had a higher z value than did strain V7 at the same storage interval. A heat treatment greater than the 4-D process recommended by the U.S. Department of Agriculture was required to inactivate the large numbers of L. monocytogenes that developed in chicken gravy during refrigerated storage.

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