Allelic Variation at Glutenin Subunit and Gliadin Loci, Glu-1, Glu-3 and Gli-1, of Common Wheats. I. Its Additive and Interaction Effects on Dough Properties

The low-molecular weight glutenin subunit (LMW-GS) composition of wheat (Triticum aestivum) flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. In this study, we used an optimized reversed-phase HPLC method and proteomics approach comprising 2-D gels coupled with liquid chromatography–tandem mass spectrometry (MS/MS) to discriminate individual LMW-GSs corresponding to alleles encoded by the Glu-A3, Glu-B3, and Glu-D3 loci in the ‘Aroona’ cultivar and 12 ‘Aroona’ near-isogenic lines (ARILs), which contain unique LMW-GS alleles in the same genetic background. The LMW-GS separation patterns for ‘Aroona’ and ARILs on chromatograms and 2-D gels were consistent with those from a set of 10 standard wheat cultivars for Glu-3. Furthermore, 12 previously uncharacterized spots in ‘Aroona’ and ARILs were excised from 2-D gels, digested with chymotrypsin, and subjected to MS/MS. We identified their gene haplotypes and created a 2-D gel map of LMW-GS alleles in the germplasm for breeding and screening for desirable LMW-GS alleles for wheat quality improvement.

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Allelic variation and genetic diversity of high molecular weight glutenin subunit in Chinese endemic wheats (Triticum aestivum L.)

Euphytica ◽

10.1007/s10681-008-9812-4 ◽

2008 ◽

Vol 166 (2) ◽

pp. 177-182 ◽

Cited By ~ 15

Author(s):

Jingye Fang ◽

Yong Liu ◽

Jie Luo ◽

Yuesheng Wang ◽

Peter R. Shewry ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Weight ◽

Triticum Aestivum ◽

High Molecular Weight ◽

Allelic Variation ◽

Triticum Aestivum L ◽

Glutenin Subunit

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The association between high molecular weight glutenin subunit compositions and the bread-making quality of Chinese and Japanese hexaploid wheats

Australian Journal of Agricultural Research ◽

10.1071/ar99065 ◽

2000 ◽

Vol 51 (3) ◽

pp. 371 ◽

Cited By ~ 1

Author(s):

H. Nakamura

Keyword(s):

Molecular Weight ◽

Genetic Variation ◽

High Molecular Weight ◽

Hexaploid Wheat ◽

Allelic Variation ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Glutenin Subunit ◽

Wheat Varieties ◽

Hmw Glutenin

Variation in the electrophoretic banding patterns of high molecular weight (HMW) glutenin subunits of 274 hexaploid wheat (Triticum aestivum) varieties from China was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Twenty-seven different major glutenin HMW subunits were identified. Each variety contained 3–5 subunits and 29 different glutenin subunit patterns were segregated. Seventeen alleles were identified based on comparison of subunit mobilities with those previously found for hexaploid wheat. Chinese hexaploid wheats exhibited particular allelic variation in glutenin HMW subunit composition and this variation differed from that found in wheats from Japanese and other countries. Average Glu-1 quality scores of 274 Chinese wheat varieties in the present study have been shown to be higher than that of Japanese wheats. Considerable genetic variation in the HMW glutenin subunit compositions of the Chinese wheats was observed in the present study and previously. Alleles from Chinese hexaploid wheat varieties have not been extensively introduced into Japan and other countries. The present data may indicate possible applications of Chinese germplasm in wheat breeding programs. To improve the wheat quality, genetic variation should be attempted through the introduction of genes of Chinese varieties into varieties in Japan and other countries.

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The high-molecular-weight glutenin subunit composition of Japanese hexaploid wheat landraces

Australian Journal of Agricultural Research ◽

10.1071/ar99155 ◽

2000 ◽

Vol 51 (6) ◽

pp. 673 ◽

Cited By ~ 8

Author(s):

H. Nakamura

Keyword(s):

Molecular Weight ◽

High Molecular Weight ◽

Hexaploid Wheat ◽

Storage Proteins ◽

Allelic Variation ◽

Polyacrylamide Gel Electrophoresis ◽

Sodium Dodecyl ◽

Glutenin Subunit ◽

Subunit Composition ◽

Wheat Landraces

The endosperm storage proteins of 174 Japanese wheat (Triticum aestivum) landraces were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis to determine their high-molecular-weight (HMW) glutenin subunit composition. These are alleles for complex gene loci, Glu-A1, Glu-B1, and Glu-D1, that are present in Japanese hexaploid wheat landraces. These were identified by comparison with the subunit mobility previously found in hexaploid wheat. Twenty-four different, major glutenin HMW subunits were identified. Each landrace contained 3–5 subunits, and 17 different glutenin subunit patterns were observed for 13 alleles in Japanese landraces. Japanese landraces showed specific allelic variation in glutenin HMW subunits, different from those in non-Japanese hexaploid wheats.

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