Macromolecular Assemblage of Aminoacyl-tRNA Synthetases: Quantitative Analysis of Protein-Protein Interactions and Mechanism of Complex Assembly

2000 ◽  
Vol 304 (5) ◽  
pp. 983-994 ◽  
Author(s):  
Jean-Charles Robinson ◽  
Pierre Kerjan ◽  
Marc Mirande
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Complex Assembly ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases

scholarly journals Heat Shock Protein 90 Mediates Protein-protein Interactions between Human Aminoacyl-tRNA Synthetases

2000 ◽  
Vol 275 (41) ◽  
pp. 31682-31688 ◽  
Author(s):  
Jeongwoo Kang ◽  
Taeho Kim ◽  
Young-Gyu Ko ◽  
Seung Bae Rho ◽  
Sang Gyu Park ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Interactions ◽  
Heat Shock Protein 90 ◽  
Protein Protein Interactions ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases

Macromolecular assemblage of aminoacyl-tRNA synthetases: identification of protein-protein interactions and characterization of a core protein 1 1Edited by J. Karn

1999 ◽  
Vol 285 (1) ◽  
pp. 183-195 ◽  
Author(s):  
Sophie Quevillon ◽  
Jean-Charles Robinson ◽  
Eric Berthonneau ◽  
Miroslawa Siatecka ◽  
Marc Mirande
Keyword(s):  
Protein Interactions ◽  
Core Protein ◽  
Protein Protein Interactions ◽  
Trna Synthetases ◽  
Aminoacyl Trna Synthetases

scholarly journals Androgen signaling uses a writer and a reader of ADP-ribosylation to regulate protein complex assembly

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Chun-Song Yang ◽  
Kasey Jividen ◽  
Teddy Kamata ◽  
Natalia Dworak ◽  
Luke Oostdyk ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Interactions ◽  
Prostate Cancer Cells ◽  
Protein Protein Interactions ◽  
Post Translational Modification ◽  
Complex Assembly ◽  
Adp Ribosylation ◽  
Signaling Axis ◽  
Regulate Protein ◽  
Protein Complex Assembly

AbstractAndrogen signaling through the androgen receptor (AR) directs gene expression in both normal and prostate cancer cells. Androgen regulates multiple aspects of the AR life cycle, including its localization and post-translational modification, but understanding how modifications are read and integrated with AR activity has been difficult. Here, we show that ADP-ribosylation regulates AR through a nuclear pathway mediated by Parp7. We show that Parp7 mono-ADP-ribosylates agonist-bound AR, and that ADP-ribosyl-cysteines within the N-terminal domain mediate recruitment of the E3 ligase Dtx3L/Parp9. Molecular recognition of ADP-ribosyl-cysteine is provided by tandem macrodomains in Parp9, and Dtx3L/Parp9 modulates expression of a subset of AR-regulated genes. Parp7, ADP-ribosylation of AR, and AR-Dtx3L/Parp9 complex assembly are inhibited by Olaparib, a compound used clinically to inhibit poly-ADP-ribosyltransferases Parp1/2. Our study reveals the components of an androgen signaling axis that uses a writer and reader of ADP-ribosylation to regulate protein-protein interactions and AR activity.

scholarly journals Engineering bromodomains with a photoactive amino acid by engaging ‘Privileged’ tRNA synthetases

2020 ◽  
Vol 56 (25) ◽  
pp. 3641-3644
Author(s):  
Shana Wagner ◽  
Babu Sudhamalla ◽  
Philip Mannes ◽  
Sushma Sappa ◽  
Sam Kavoosi ◽  
...  
Keyword(s):  
Amino Acid ◽  
Chemical Synthesis ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
Site Specific ◽  
Trna Synthetases

An improved chemical synthesis, site-specific incorporation and enhanced photo-crosslinking ability of tmdF have been demonstrated in the context of protein–protein interactions.

Micropatterning for quantitative analysis of protein-protein interactions in living cells

2008 ◽  
Vol 5 (12) ◽  
pp. 1053-1060 ◽  
Author(s):  
Michaela Schwarzenbacher ◽  
Martin Kaltenbrunner ◽  
Mario Brameshuber ◽  
Clemens Hesch ◽  
Wolfgang Paster ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions

scholarly journals Quantitative analysis of dynamic protein-protein interactions in planta by a floated-leaf luciferase complementation imaging (FLuCI) assay using binary Gateway vectors

2011 ◽  
Vol 67 (3) ◽  
pp. 542-553 ◽  
Author(s):  
Christian Gehl ◽  
David Kaufholdt ◽  
Domenica Hamisch ◽  
Rolf Bikker ◽  
Jörg Kudla ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
In Planta ◽  
Protein Protein Interactions ◽  
Gateway Vectors

scholarly journals Highly Modular Protein Micropatterning Sheds Light on the Role of Clathrin-Mediated Endocytosis for the Quantitative Analysis of Protein-Protein Interactions in Live Cells

Biomolecules ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 540 ◽  
Author(s):  
Peter Lanzerstorfer ◽  
Ulrike Müller ◽  
Klavdiya Gordiyenko ◽  
Julian Weghuber ◽  
Christof M. Niemeyer
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Spatial Arrangement ◽  
Interaction Partner ◽  
Live Cells ◽  
Protein Protein Interactions ◽  
Large Area ◽  
Contact Printing ◽  
Prey Protein ◽  
Protein Micropatterning

Protein micropatterning is a powerful tool for spatial arrangement of transmembrane and intracellular proteins in living cells. The restriction of one interaction partner (the bait, e.g., the receptor) in regular micropatterns within the plasma membrane and the monitoring of the lateral distribution of the bait’s interaction partner (the prey, e.g., the cytosolic downstream molecule) enables the in-depth examination of protein-protein interactions in a live cell context. This study reports on potential pitfalls and difficulties in data interpretation based on the enrichment of clathrin, which is a protein essential for clathrin-mediated receptor endocytosis. Using a highly modular micropatterning approach based on large-area micro-contact printing and streptavidin-biotin-mediated surface functionalization, clathrin was found to form internalization hotspots within the patterned areas, which, potentially, leads to unspecific bait/prey protein co-recruitment. We discuss the consequences of clathrin-coated pit formation on the quantitative analysis of relevant protein-protein interactions, describe controls and strategies to prevent the misinterpretation of data, and show that the use of DNA-based linker systems can lead to the improvement of the technical platform.

Quantitative Analysis of Protein–Protein Interactions by Native Page/Fluorimaging

2005 ◽  
Vol 15 (4) ◽  
pp. 469-473 ◽  
Author(s):  
Kylie M. Wagstaff ◽  
Manisha M. Dias ◽  
Gualtiero Alvisi ◽  
David A. Jans
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Protein Protein Interactions ◽  
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