3D Electrochemical Sensor and Microstructuration Using Aerosol Jet Printing

Electrochemical sensors are attracting great interest for their different applications. To improve their performances, basic research focuses on two main issues: improve their metrological characteristics (e.g., repeatability, reusability and sensitivity) and investigate innovative fabrication processes. In this work, we demonstrate an innovative microstructuration technique aimed at increasing electrochemical sensor sensitivity to improve electrode active area by an innovative fabrication technique. The process is empowered by aerosol jet printing (AJP), an additive-manufacturing and non-contact printing technique that allows depositing functional inks in precise patterns such as parallel lines and grids. The 3D printed microstructures increased the active surface area by up to 130% without changing the substrate occupancy. Further, electrochemical detection of ferro/ferri-cyanide was used to evaluate the sensitivity of the electrodes. This evaluation points out a sensitivity increase of 2.3-fold on average between bare and fully microstructured devices. The increase of surface area and sensitivity are well linearly correlated as expected, verifying the fitness of our production process. The proposed microstructuration is a viable solution for many applications that requires high sensitivity, and the proposed technique, since it does not require masks or complex procedures, turns out to be flexible and applicable to infinite construction geometries.

Development of a highly controlled system for large-area, directional printing of quasi-1D nanomaterials

Printing is a promising method for the large-scale, high-throughput, and low-cost fabrication of electronics. Specifically, the contact printing approach shows great potential for realizing high-performance electronics with aligned quasi-1D materials. Despite being known for more than a decade, reports on a precisely controlled system to carry out contact printing are rare and printed nanowires (NWs) suffer from issues such as location-to-location and batch-to-batch variations. To address this problem, we present here a novel design for a tailor-made contact printing system with highly accurate control of printing parameters (applied force: 0–6 N ± 0.3%, sliding velocity: 0–200 mm/s, sliding distance: 0–100 mm) to enable the uniform printing of nanowires (NWs) aligned along 93% of the large printed area (1 cm2). The system employs self-leveling platforms to achieve optimal alignment between substrates, whereas the fully automated process minimizes human-induced variation. The printing dynamics of the developed system are explored on both rigid and flexible substrates. The uniformity in printing is carefully examined by a series of scanning electron microscopy (SEM) images and by fabricating a 5 × 5 array of NW-based photodetectors. This work will pave the way for the future realization of highly uniform, large-area electronics based on printed NWs.

Development of a Lateral Flow Immunoassay (LFIA) to Screen for the Release of the Endocrine Disruptor Bisphenol A from Polymer Materials and Products

One of the most important chemicals used in the production of polymer plastics and coatings is bisphenol A. However, despite the large number of studies on the toxicity and hormonal activity of BPA, there are still open questions and thus considerable media attention regarding BPA toxicity. Hence, it is necessary to develop a sensitive, simple, cost-efficient, specific, portable, and rapid method for monitoring bisphenol A and for high sample throughput and on-site screening analysis. Lateral flow immunoassays have potential as rapid tests for on-site screening. To meet sensitivity criteria, they must be carefully optimized. A latex microparticle-based LFIA for detection of BPA was developed. The sensitivity of the assay was improved by non-contact printing of spot grids as the control and test lines with careful parameter optimization. Results of the test could be visually evaluated within 10 min with a visual cut-off of 10 µg/L (vLOD). Alternatively, photographs were taken, and image analysis performed to set up a calibration, which allowed for a calculated limit of detection (cLOD) of 0.14 µg/L. The method was validated for thermal paper samples against ELISA and LC–MS/MS as reference methods, showing good agreement with both methods.

Rapid fabrication of hydrogel micropatterns by projection stereolithography for studying self-organized developmental patterning

Self-organized patterning of mammalian embryonic stem cells on micropatterned surfaces has previously been established as an in vitro platform for early mammalian developmental studies, complimentary to in vivo studies. Traditional micropatterning methods, such as micro-contact printing (μCP), involve relatively complicated fabrication procedures, which restricts widespread adoption by biologists. Here, we demonstrate a rapid method of micropatterning by printing hydrogel micro-features onto a glass-bottomed culture vessel. The micro-features are printed using a projection stereolithography bioprinter yielding hydrogel structures that geometrically restrict the attachment of cells or proteins. Compared to traditional and physical photomasks, a digitally tunable virtual photomask is used in the projector to generate blue light patterns that enable rapid iteration with minimal cost and effort. We show that a protocol that makes use of this method together with LN521 coating, an extracellular matrix coating, creates a surface suitable for human embryonic stem cell (hESC) attachment and growth with minimal non-specific adhesion. We further demonstrate that self-patterning of hESCs following previously published gastrulation and ectodermal induction protocols achieves results comparable with those obtained with commercially available plates.

A model study on controlling dealloying corrosion attack by lateral modification of surfactant inhibitors

Detrimental corrosion is an ever-concerning challenge for metals and alloys. One possible remedy is to apply organic corrosion inhibitors. Despite progress in molecular assembly and inhibitor research, better mechanistic insight on the molecular level is needed. Here we report on the behavior of well-defined artificial molecular interfaces created by micro-contact printing of thiol-inhibitor molecules and subsequent backfilling. The obtained heterogeneity and defects trigger localized dealloying-corrosion of well-defined Cu3Au surfaces. The stability of applied inhibitor molecules depends on alloy surface morphology and on intermolecular forces of the molecular layers. On extended terraces, dealloying preferentially starts at the boundary between areas composed of the two different chain-length inhibitor molecules. Inside of the areas hardly any nucleation of initial pits is visible. Step density strongly influences the morphology of the dealloying attack, while film heterogeneity avoids cracking and controls molecular-scale corrosion attack. The presented surface-science approach, moreover, will ultimately allow to verify the acting mechanisms of inhibitor-cocktails to develop recipes to stabilize metallic alloy surfaces.

A full catalogue and analysis of Indian painted photographs at Royal Ontario Museum's South Asian photographic collection

This thesis is based on a photographic collection of Indian painted photographs from the South Asian Photographic collection at the Royal Ontario Museum (ROM). There are fifty-four photographic objects created by various makers and photographers with dates ranging from the 1880's to the 1990's. For the most part the objects are examples of studio portraiture. Most of them are photographic images with applied colour, however, there are some examples of paintings in this group that were executed in the tradition of photographic studio portraiture, but have no evidence of a photosensitive material underneath. The paintings, as well as the painted photographs, employ different media, such as watercolour, gouache and oil paints. The objects I investigated and catalogued fall under three categories: prints made by contact printing, by enlargement, and finally paintings produced using a photograph as a model. Tinting of photographs was a well-known Western tradition in the nineteenth century, while the process that Indian artists developed was a synthesis of their long practiced tradition of miniature painting and the newly developed technology of photography. Finally this thesis unveils the means of production of Indian painted photographs, and tries to find the reason for Indian artists employing opaque medium in their colourings.

A full catalogue and analysis of Indian painted photographs at Royal Ontario Museum's South Asian photographic collection

This thesis is based on a photographic collection of Indian painted photographs from the South Asian Photographic collection at the Royal Ontario Museum (ROM). There are fifty-four photographic objects created by various makers and photographers with dates ranging from the 1880's to the 1990's. For the most part the objects are examples of studio portraiture. Most of them are photographic images with applied colour, however, there are some examples of paintings in this group that were executed in the tradition of photographic studio portraiture, but have no evidence of a photosensitive material underneath. The paintings, as well as the painted photographs, employ different media, such as watercolour, gouache and oil paints. The objects I investigated and catalogued fall under three categories: prints made by contact printing, by enlargement, and finally paintings produced using a photograph as a model. Tinting of photographs was a well-known Western tradition in the nineteenth century, while the process that Indian artists developed was a synthesis of their long practiced tradition of miniature painting and the newly developed technology of photography. Finally this thesis unveils the means of production of Indian painted photographs, and tries to find the reason for Indian artists employing opaque medium in their colourings.