Purification of α-Sarcin and an Antifungal Protein fromAspergillus giganteusby Blue Sepharose CL-6B Affinity Chromatography

1998 ◽  
Vol 14 (2) ◽  
pp. 295-301 ◽  
Author(s):  
Jian-Jiang Hao ◽  
Yong-zhen Xu ◽  
Chuan-dong Geng ◽  
Wang-Yi Liu ◽  
En-duo Wang ◽  
...  
Keyword(s):  
Affinity Chromatography ◽  
Antifungal Protein ◽  
Blue Sepharose

Dye affinity chromatography of ricin subunits

1986 ◽  
Vol 6 (12) ◽  
pp. 1035-1040 ◽  
Author(s):  
Simonetta Sperti ◽  
Lucio Montanaro ◽  
Fioretta Rambelli
Keyword(s):  
Affinity Chromatography ◽  
Simple Method ◽  
Blue Dextran ◽  
A Chain ◽  
Dye Affinity Chromatography ◽  
Blue Sepharose

A rapid and simple method for the isolation of the two chains of ricin is described. The method involves two chromatographic runs, the first on blue-Sepharose and the second on blue dextran-Sepharose. It is moreover shown that the A chain of ricin, but not the B chain, binds to poly(U)-Sepharose.

Mitogenic activity of foetal calf serum is completely removed by affinity chromatography using Blue Sepharose

1986 ◽  
Vol 14 (6) ◽  
pp. 1088-1088
Author(s):  
PAMELA O. LAMINA ◽  
PETER KNOX
Keyword(s):  
Affinity Chromatography ◽  
Calf Serum ◽  
Mitogenic Activity ◽  
Foetal Calf Serum ◽  
Blue Sepharose

scholarly journals Identification of albumin as the plasma carrier for zinc absorption by perfused rat intestine

1979 ◽  
Vol 184 (3) ◽  
pp. 627-633 ◽  
Author(s):  
K T Smith ◽  
M L Failla ◽  
R J Cousins
Keyword(s):  
Affinity Chromatography ◽  
Gel Electrophoresis ◽  
Serum Zinc ◽  
Zinc Binding ◽  
Zinc Absorption ◽  
Rat Intestine ◽  
Protein Carrier ◽  
Mucosal Cells ◽  
Intestinal Mucosal Cells ◽  
Blue Sepharose

The isolated vascularly perfused rat intestine exhibits an obligatory need for a protein carrier in order to absorb zinc. Therefore this system is ideal for use as a model to identify the plasma carrier during zinc absorption. Affinity chromatography on Blue Sepharose CL-6B was employed to separate the major serum zinc-binding proteins in the portal effluent of the perfused intestine. It was found that 94% of newly absorbed 65Zn was transported in the portal serum-containing perfusate as an albumin-65Zn complex. The identity of albumin as the plasma carrier was confirmed by polyacrylamide-slab-gel electrophoresis. This evidence suggests that albumin is the plasma protein that is involved in removal of zinc from intestinal-mucosal cells and subsequent transport of the metal in portal blood to the liver.

scholarly journals Affinity chromatography of endo-1,4-.BETA.-xylanase from mid-gut gland of apple snail on blue sepharose CL-6B.

1989 ◽  
Vol 53 (10) ◽  
pp. 2809-2810
Author(s):  
Manpei SUZUKI ◽  
Katsuro ASHIDA
Keyword(s):  
Affinity Chromatography ◽  
Apple Snail ◽  
Blue Sepharose

Purification of d-amino acid oxidase apoenzyme by affinity chromatography on Cibacron Blue Sepharose

1985 ◽  
Vol 347 ◽  
pp. 316-319 ◽  
Author(s):  
Joëlle Léonil ◽  
Sylvie Langrené ◽  
Sames Sicsic ◽  
François Le Goffic
Keyword(s):  
Amino Acid ◽  
Affinity Chromatography ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Cibacron Blue ◽  
Blue Sepharose

scholarly journals Enzymic synthesis of sym-homospermidine in Lathyrus sativus (grass pea) seedlings

1980 ◽  
Vol 190 (2) ◽  
pp. 461-464 ◽  
Author(s):  
K S Srivenugopal ◽  
P R Adiga
Keyword(s):  
Enzyme Activity ◽  
Affinity Chromatography ◽  
Specific Activity ◽  
Lathyrus Sativus ◽  
Santalum Album ◽  
Grass Pea ◽  
Enzymic Synthesis ◽  
Crude Extracts ◽  
Pea Seedlings ◽  
Blue Sepharose

An enzyme catalysing the synthesis of sym-homospermidine from putrescine and NAD+ with concomitant liberation of NH3 was purified 100-fold from Lathyrus sativus (grass pea) seedlings by affinity chromatography on Blue Sepharose. This thiol enzyme had an apparent mol.wt. of 75000 and exhibited Michelis-Menten kinetics with Km 3.0mM for putrescine. The same enzyme activity could also be demonstrated in the crude extracts of sandal (Santalum album) leaves, but with a specific activity 15-fold greater than that in L. sativus seedlings.

scholarly journals Purification of .BETA.-lactamase from Streptomyces cellulosae by affinity chromatography on blue sepharose.

1979 ◽  
Vol 32 (12) ◽  
pp. 1328-1335 ◽  
Author(s):  
HIROSHI OGAWARA ◽  
SUSUMU HORIKAWA
Keyword(s):  
Affinity Chromatography ◽  
Beta Lactamase ◽  
Blue Sepharose
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