Identification of a High-Molecular-Weight Cellular Protein Complex Containing the Adenovirus DNA Binding Protein

Virology ◽  
1994 ◽  
Vol 202 (2) ◽  
pp. 715-723 ◽  
Author(s):  
Joseph W. Ricigliano ◽  
Douglas E. Brough ◽  
Daniel F. Klessig
Keyword(s):  
Molecular Weight ◽  
Dna Binding ◽  
High Molecular Weight ◽  
Protein Complex ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Cellular Protein

Single-Stranded DNA-Binding Protein Complex from Helicobacter pylori Suggests an ssDNA-Binding Surface

2009 ◽  
Vol 388 (3) ◽  
pp. 508-519 ◽  
Author(s):  
Kun-Wei Chan ◽  
Yi-Juan Lee ◽  
Chia-Hung Wang ◽  
Haimei Huang ◽  
Yuh-Ju Sun
Keyword(s):  
Helicobacter Pylori ◽  
Dna Binding ◽  
Protein Complex ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Stranded Dna ◽  
Ssdna Binding ◽  
Binding Surface

scholarly journals Interaction between anti-DNA and anti-DNA-binding protein autoantibodies in cryoglobulins from sera of patients with systemic lupus erythematosus.

1986 ◽  
Vol 164 (4) ◽  
pp. 1029-1042 ◽  
Author(s):  
W H Reeves ◽  
N Chiorazzi
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Dna Binding ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Protein Complex ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Systemic Lupus ◽  
Variable Regions ◽  
Dna Antibodies

We have previously shown that sera from some patients with SLE and related disorders contain autoantibodies to a DNA-binding protein complex designated p70/p80. The present study shows that anti-p70/p80 autoantibodies are frequently accompanied by anti-DNA antibodies and cryoglobulins. When the cryoglobulins were isolated, they were found to be specifically enriched in both anti-p70/p80 and anti-DNA activities. The anti-p70/p80 and anti-DNA antibodies were found to be distinct populations of autoantibodies rather than a single crossreactive species, since they could be separated from one another by chromatography on DNA-cellulose. Certain human anti-DNA mAbs could inhibit the binding of autoimmune polyclonal anti-p70/p80 antibodies to p70/p80, suggesting that anti-DNA antibodies might also associate with the variable regions of some anti-p70/p80 antibodies in the cryoglobulins. Binding of one murine anti-p70/p80 mAb (111-12) also was inhibited by certain human anti-DNA mAbs, but the binding of another murine mAb (162-11) to a different epitope of p70/p80 was not. These studies suggest that certain anti-DNA antibodies may interact with the variable regions of a population of anti-p70/p80 antibodies. The cryoglobulins found in the sera containing both anti-p70/p80 and anti-DNA antibodies may represent immune complexes consisting, in part, of idiotype and antiidiotype.

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