Induced Systemic Resistance as a Mechanism of Disease Suppression by Rhizobacteria

2005 ◽  
pp. 39-66 ◽  
Author(s):  
L.C. Van Loon ◽  
P.A.H.M. Bakker
Keyword(s):  
Induced Systemic Resistance ◽  
Disease Suppression ◽  
Systemic Resistance

scholarly journals Evidence of Induced Systemic Resistance Against Botrytis elliptica in Lily

2008 ◽  
Vol 98 (7) ◽  
pp. 830-836 ◽  
Author(s):  
Yi-Hung Liu ◽  
Chien-Jui Huang ◽  
Chao-Ying Chen
Keyword(s):  
Induced Systemic Resistance ◽  
Disease Incidence ◽  
Field Studies ◽  
Defense Responses ◽  
Disease Suppression ◽  
Systemic Resistance ◽  
Fatty Acid Profiles ◽  
Plant Defense Responses ◽  
Gene Expression Analyses ◽  
Glycine Rich Protein

Lily leaf blight, caused by Botrytis elliptica, is an important fungal disease in Taiwan. In order to identify an effective, nonfungicide method to decrease disease incidence in Lilium formosanum, the efficacy of rhizobacteria eliciting induced systemic resistance (ISR) was examined in this study. Over 300 rhizobacteria were isolated from the rhizosphere of L. formosanum healthy plants and 63 were identified by the analysis of fatty acid profiles. Disease suppressive ability of 13 strains was demonstrated by soil drench application of bacterial suspensions to the rhizosphere of L. formosanum seedlings. Biocontrol experiments were carried out with Bacillus cereus and Pseudomonas putida strains on L. formosanum and Lilium Oriental hybrid cvs. Acapulco and Star Gazer in greenhouse and field studies. Plants treated with B. cereus strain C1L showed that protection against B. elliptica on L. formosanum could last for at least 10 days and was consistent with high populations of B. cereus on lily roots. Analysis of the expression of LfGRP1 and LsGRP1, encoding glycine-rich protein associated with L. formosanum and cv. Star Gazer, respectively, revealed different responses induced by B. cereus or by the pathogen B. elliptica, suggesting that plant defense responses elicited by each follows a different signaling pathway. According to the results of biocontrol assays and LfGRP1/LsGRP1 gene expression analyses with culture filtrates of B. cereus strain C1L, we propose that eliciting factors of ISR are generated by B. cereus and some of them exhibit thermostable and heat-tolerant traits. This is the first report about ISR-eliciting rhizobacteria and factors effective for foliar disease suppression in lily.

scholarly journals Control of Fusarium Wilt of Radish by Combining Pseudomonas putida Strains that have Different Disease-Suppressive Mechanisms

2003 ◽  
Vol 93 (5) ◽  
pp. 626-632 ◽  
Author(s):  
Marjan de Boer ◽  
Peter Bom ◽  
Frodo Kindt ◽  
Joost J. B. Keurentjes ◽  
Ientse van der Sluis ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Fusarium Wilt ◽  
Plant Pathogens ◽  
Induced Systemic Resistance ◽  
Disease Suppression ◽  
Systemic Resistance ◽  
Single Application ◽  
Multiple Traits ◽  
Additional Mechanism ◽  
Single Strain

Biological control of soilborne plant pathogens in the field has given variable results. By combining specific strains of microorganisms, multiple traits antagonizing the pathogen can be combined and this may result in a higher level of protection. Pseudomonas putida WCS358 suppresses Fusarium wilt of radish by effectively competing for iron through the production of its pseudobactin siderophore. However, in some bioassays pseudobactin-negative mutants of WCS358 also suppressed disease to the same extent as WCS358, suggesting that an, as yet unknown, additional mechanism may be operative in this strain. P. putida strain RE8 induced systemic resistance against fusarium wilt. When WCS358 and RE8 were mixed through soil together, disease suppression was significantly enhanced to approximately 50% as compared to the 30% reduction for the single strain treatments. Moreover, when one strain failed to suppress disease in the single application, the combination still resulted in disease control. The enhanced disease suppression by the combination of P. putida strains WCS358 and RE8 is most likely the result of the combination of their different disease-suppressive mechanisms. These results demonstrate that combining biocontrol strains can lead to more effective, or at least, more reliable biocontrol of fusarium wilt of radish.

scholarly journals Role of Iron in Rhizobacteria-Mediated Induced Systemic Resistance of Cucumber

2001 ◽  
Vol 91 (6) ◽  
pp. 593-598 ◽  
Author(s):  
C. M. Press ◽  
J. E. Loper ◽  
J. W. Kloepper
Keyword(s):  
Serratia Marcescens ◽  
Induced Systemic Resistance ◽  
Iron Concentration ◽  
Iron Chelator ◽  
Disease Suppression ◽  
Systemic Resistance ◽  
Wild Type ◽  
Rhizosphere Bacterium ◽  
Population Sizes ◽  
External Population

Seed treatment with the rhizosphere bacterium Serratia marcescens strain 90-166 suppressed anthracnose of cucumber, caused by Colleto-trichum orbiculare, through induced systemic resistance (ISR). When the iron concentration of a planting mix was decreased by addition of an iron chelator, suppression of cucumber anthracnose by strain 90-166 was significantly improved. Strain 90-166 produced 465 ± 70 mg/liter of catechol siderophore, as determined by the Rioux assay in deferrated King's medium B. The hypothesis that a catechol siderophore produced by strain 90-166 may be responsible for induction of systemic resistance by this strain was tested by evaluating disease suppression by a mini-Tn5-phoA mutant deficient in siderophore production. Sequence analysis of genomic DNA flanking the mini-Tn5-phoA insertion identified the target gene as entA, which encodes an enzyme in the catechol siderophore biosynthetic pathways of several bacteria. Severity of anthracnose of cucumbers treated with the entA mutant was not significantly different (P = 0.05) from the control, whereas plants treated with wild-type 90-166 had significantly less disease (P = 0.05) than the control. Total (internal and external) population sizes of 90-166 and the entA mutant on roots did not differ significantly (P = 0.05) at any sample time, whereas internal population sizes of the entA mutant were significantly lower (P = 0.05) than those of the wild-type strain at two sampling times. These data suggest that catechol siderophore biosynthesis genes in Serratia marcescens 90-166 are associated with ISR but that this role may be indirect via a reduction in internal root populations.

scholarly journals Induced Systemic Resistance by Fluorescent Pseudomonas spp.

2007 ◽  
Vol 97 (2) ◽  
pp. 239-243 ◽  
Author(s):  
Peter A. H. M. Bakker ◽  
Corné M. J. Pieterse ◽  
L. C. van Loon
Keyword(s):  
Induced Systemic Resistance ◽  
Plant Diseases ◽  
Disease Suppression ◽  
Systemic Resistance ◽  
Lytic Enzymes ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Two Populations

Fluorescent Pseudomonas spp. have been studied for decades for their plant growth-promoting effects through effective suppression of soilborne plant diseases. The modes of action that play a role in disease suppression by these bacteria include siderophore-mediated competition for iron, antibiosis, production of lytic enzymes, and induced systemic resistance (ISR). The involvement of ISR is typically studied in systems in which the Pseudomonas bacteria and the pathogen are inoculated and remain spatially separated on the plant, e.g., the bacteria on the root and the pathogen on the leaf, or by use of split root systems. Since no direct interactions are possible between the two populations, suppression of disease development has to be plant-mediated. In this review, bacterial traits involved in Pseudomonas-mediated ISR will be discussed.

scholarly journals Bacillus Cyclic Lipopeptides Iturin and Fengycin Control Rice Blast Caused by Pyricularia oryzae in Potting and Acid Sulfate Soils by Direct Antagonism and Induced Systemic Resistance

2021 ◽  
Vol 9 (7) ◽  
pp. 1441
Author(s):  
Van Bach Lam ◽  
Thibault Meyer ◽  
Anthony Arguelles Arias ◽  
Marc Ongena ◽  
Feyisara Eyiwumi Oni ◽  
...  
Keyword(s):  
Rice Blast ◽  
Induced Systemic Resistance ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Systemic Resistance ◽  
Acid Sulfate Soils ◽  
Acid Sulfate ◽  
Wide Range ◽  
Bacillus Spp ◽  
Sulfate Soils

Rice monoculture in acid sulfate soils (ASSs) is affected by a wide range of abiotic and biotic constraints, including rice blast caused by Pyricularia oryzae. To progress towards a more sustainable agriculture, our research aimed to screen the biocontrol potential of indigenous Bacillus spp. against blast disease by triggering induced systemic resistance (ISR) via root application and direct antagonism. Strains belonging to the B. altitudinis and B. velezensis group could protect rice against blast disease by ISR. UPLC–MS and marker gene replacement methods were used to detect cyclic lipopeptide (CLiP) production and construct CLiPs deficient mutants of B. velezensis, respectively. Here we show that the CLiPs fengycin and iturin are both needed to elicit ISR against rice blast in potting soil and ASS conditions. The CLiPs surfactin, iturin and fengycin completely suppressed P. oryzae spore germination resulting in disease severity reduction when co-applied on rice leaves. In vitro microscopic assays revealed that iturin and fengycin inhibited the mycelial growth of the fungus P. oryzae, while surfactin had no effect. The capacity of indigenous Bacillus spp. to reduce rice blast by direct and indirect antagonism in ASS conditions provides an opportunity to explore their usage for rice blast control in the field.

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