Genetic Variation in the Timing of Larval Mortality and Plant Tissue Responses Associated with Tree Resistance against Galling Adelgids

To distinguish the taxa at the specific and varietal levels a range of DNA fingerprinting techniques are being employed. The objective of the current study was to establish the genetic correlation between the Trigonella-Melilotus complex i.e. M. indica, M. alba and Trigonella polyceratia using ten Consensus Chloroplast Microsatellite Primers (CCMPs). The polymorphism between the two genera indicated that they are intimately related and symbolize novel incongruity. Owing to less significant level of genetic variation, detected through CCMP, the differences between the two genera could be accredited to gene mutation or inconsequential chromosomal alterations. Plant Tissue Cult. & Biotech. 23(1): 59-66, 2013 (June)

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Regeneration and Genetic Variation in Plant Tissue Cultures

Clonal Forestry I ◽

10.1007/978-3-642-84175-0_7 ◽

1993 ◽

pp. 87-100 ◽

Cited By ~ 3

Author(s):

Z. Chen ◽

M. R. Ahuja

Keyword(s):

Genetic Variation ◽

Plant Tissue ◽

Tissue Cultures ◽

Plant Tissue Cultures

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Freeze-fracture, freeze-etch complementary pairs of virus-infected cells reveal value of etching even when relatively high concentrations of cryoprotectants are used

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100081541 ◽

1978 ◽

Vol 36 (2) ◽

pp. 136-137

Author(s):

Russell L. Steere ◽

Eric F. Erbe

Keyword(s):

Plant Tissue ◽

Phosphate Buffer ◽

Infected Plant ◽

Freeze Fracture ◽

Distilled Water ◽

Virus Infected Plant ◽

Infected Cells ◽

High Concentrations

It has been assumed by many involved in freeze-etch or freeze-fracture studies that it would be useless to etch specimens which were cryoprotected by more than 15% glycerol. We presumed that the amount of cryoprotective material exposed at the surface would serve as a contaminating layer and prevent the visualization of fine details. Recent unexpected freeze-etch results indicated that it would be useful to compare complementary replicas in which one-half of the frozen-fractured specimen would be shadowed and replicated immediately after fracturing whereas the complement would be etched at -98°C for 1 to 10 minutes before being shadowed and replicated.Standard complementary replica holders (Steere, 1973) with hinges removed were used for this study. Specimens consisting of unfixed virus-infected plant tissue infiltrated with 0.05 M phosphate buffer or distilled water were used without cryoprotectant. Some were permitted to settle through gradients to the desired concentrations of different cryoprotectants.

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Freeze-Fracture Studies of Membranes in Developing Sieve Elements in a Plant Tissue Culture

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002798 ◽

1980 ◽

Vol 38 ◽

pp. 636-637

Author(s):

R. D. Sjolund ◽

C. Y. Shih

Keyword(s):

Plant Tissue ◽

Cultured Cells ◽

Freeze Fracture ◽

Tissue Cultures ◽

Sieve Elements ◽

Intact Plants ◽

Plant Tissue Cultures ◽

Whole Plants ◽

Energy Dependent ◽

Similar Elements

The differentiation of phloem in plant tissue cultures offers a unique opportunity to study the development and structure of sieve elements in a manner that avoids the injury responses associated with the processing of similar elements in intact plants. Short segments of sieve elements formed in tissue cultures can be fixed intact while the longer strands occuring in whole plants must be cut into shorter lengths before processing. While iyuch controversy surrounds the question of phloem function in tissue cultures , sieve elements formed in these cultured cells are structurally similar to those of Intact plants. We are particullarly Interested In the structure of the plasma membrane and the peripheral ER in these cells because of their possible role in the energy-dependent active transport of sucrose into the sieve elements.

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