Production of Biofuel-Related Isoprenoids Derived from Botryococcus braunii Algae

2015 ◽  
pp. 141-152
Author(s):  
William A. Muzika ◽  
Nymul E. Khan ◽  
Lauren M. Jackson ◽  
Nicholas Winograd ◽  
Wayne R. Curtis
Keyword(s):  
Botryococcus Braunii

Pengaruh Senyawa Merkuri Klorida (HgCl2) Terhadap Pertumbuhan dan Kandungan Pigmen Klorofil Mikroalga Botryococcus braunii

2017 ◽  
Vol 4 (2) ◽  
pp. 23
Author(s):  
Grace Simanjuntak ◽  
Desy Mantiri ◽  
Kurniati Kemer
Keyword(s):  
Botryococcus Braunii

Penelitian ini bertujuan untuk mengetahui pertumbuhan populasi Botryococcus braunii dengan pemberian senyawa merkuri klorida (HgCl2) serta untuk mengetahui konsentrasi pigmen klorofil dari ekstrak pigmen total yang telah diberi senyawa merkuri klorida (HgCl2) terhadap mikroalga Botryococcus braunii. Stok mikroalga yang digunakan diperoleh dari Pusat Penelitian Dan Pengembangan Daya Saing Produk Dan Bioteknologi Kelautan Dan Perikanan, di Jalan KS Tubun- Pertamburan VI, Slipi, Jakarta Pusat. Stok mikroalga yang telah ada dikeluarkan dari cool box (kotak pendingin). Selanjutnya stok tersebut dibuat medium yang telah diisi oleh air laut dan media conway, setelah itu diberi aerator kemudian di kulturisasi di ruang kultur bersuhu 25oC dengan penerangan lampu tabung 40 watt selama 24 jam dan dikontrol. Kulturisasi dan perlakuan senyawa merkuri klorida dilakukan di Laboratorium Biologi Molekur dan Farmasitika Laut Fakultas Perikanan dan Ilmu Kelautan, serta untuk mengetahui panjang gelombang dan kandungan pigmennya menggunakan alat spektrofotometri dilakukan di Laboratorium Farmasi  FMIPA. Berdasarkan hasil penelitian yang telah dilakuan didapatkan senyawa merkuri klorida (HgCl2) dengan konsentrasi 2 ppm mengalami penurunan jumlah sel pada hari kedelapan, jumlah selnya 1,9 sel/ml Botryococcus braunii. Artinya senyawa yang tinggi dapat menurunkan konsentrasi pigmen sehingga menghalangi terjadinya proses fotosinteisis dengan menghambat pembelahan sel.

scholarly journals Metabolic response of Botryococcus braunii to high bicarbonate dosages and other conditions: analysis of photosynthetic performance, productivity, and lipidomic profile

2021 ◽  
Author(s):  
Néstor David Giraldo ◽  
Sandra Marcela Correa ◽  
Andrés Arbeláez ◽  
Felix L. Figueroa ◽  
Rigoberto Ríos-Estepa ◽  
...  
Keyword(s):  
Nutrient Limitation ◽  
Metabolic Response ◽  
Neutral Lipids ◽  
Biomass Productivity ◽  
Botryococcus Braunii ◽  
Photosynthetic Performance ◽  
Lipid Productivity ◽  
Cellular Growth ◽  
Adaptive Responses ◽  
Nitrogen And Phosphorus

AbstractIn this study the metabolic responses of Botryococcus braunii were analyzed upon different inorganic carbon dosages and nutrient limitation conditions in terms of lipid and biomass productivity, as well as photosynthetic performance. The nutritional schemes evaluated included different levels of sodium bicarbonate and nitrogen and phosphorus starvation, which were contrasted against standard cultures fed with CO2. Bicarbonate was found to be an advantageous carbon source since high dosages caused a significant increase in biomass and lipid productivity, in addition to an enhanced photosynthetic quantum yield and neutral lipids abundance. This contrasts to the commonly used approach of microalgae nutrient limitation, which leads to high lipid accumulation at the expense of impaired cellular growth, causing a decline in overall lipid productivity. The lipidome analysis served to hypothesize about the influence of the nutritional context on B. braunii structural and storage lipid metabolism, besides the adaptive responses exhibited by cells that underwent nutrient stress.

scholarly journals Large-scale screening of natural genetic resource in the hydrocarbon-producing microalga Botrycoccus braunii identified novel fast-growing strains

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Koji Kawamura ◽  
Suzune Nishikawa ◽  
Kotaro Hirano ◽  
Ardianor Ardianor ◽  
Rudy Agung Nugroho ◽  
...  
Keyword(s):  
Large Scale ◽  
Biomass Productivity ◽  
Botryococcus Braunii ◽  
Algal Biofuel ◽  
Fast Growing ◽  
Wild Strains ◽  
Glass Tubes ◽  
The Tropics ◽  
Large Scale Screening ◽  
Hydrocarbon Productivity

AbstractAlgal biofuel research aims to make a renewable, carbon–neutral biofuel by using oil-producing microalgae. The freshwater microalga Botryococcus braunii has received much attention due to its ability to accumulate large amounts of petroleum-like hydrocarbons but suffers from slow growth. We performed a large-scale screening of fast-growing strains with 180 strains isolated from 22 ponds located in a wide geographic range from the tropics to cool-temperate. A fast-growing strain, Showa, which recorded the highest productivities of algal hydrocarbons to date, was used as a benchmark. The initial screening was performed by monitoring optical densities in glass tubes and identified 9 wild strains with faster or equivalent growth rates to Showa. The biomass-based assessments showed that biomass and hydrocarbon productivities of these strains were 12–37% and 11–88% higher than that of Showa, respectively. One strain, OIT-678 established a new record of the fastest growth rate in the race B strains with a doubling time of 1.2 days. The OIT-678 had 36% higher biomass productivity, 34% higher hydrocarbon productivity, and 20% higher biomass density than Showa at the same cultivation conditions, suggesting the potential of the new strain to break the record for the highest productivities of hydrocarbons.

Botryoxanthin B and α-botryoxanthin A from the green microalga Botryococcus braunii Kawaguchi-1

1998 ◽  
Vol 47 (6) ◽  
pp. 1111-1115 ◽  
Author(s):  
Shigeru Okada ◽  
Izumi Tonegawa ◽  
Hisashi Matsuda ◽  
Masahiro Murakami ◽  
Katsumi Yamaguchi
Keyword(s):  
Botryococcus Braunii ◽  
Green Microalga

Enhancement of Hydrocarbon Productivity of Botryococcus braunii by an Adenine Type Phytohormone

2012 ◽  
Vol 512-515 ◽  
pp. 397-400
Author(s):  
Jun Zhi Liu ◽  
Ya Ming Ge ◽  
Guang Ming Tian
Keyword(s):  
Growth Rate ◽  
Low Dose ◽  
Algal Growth ◽  
Botryococcus Braunii ◽  
Biofuel Production ◽  
Carotenoid Content ◽  
Algae Biofuels ◽  
Hydrocarbon Content ◽  
Algal Growth Rate ◽  
Hydrocarbon Productivity

This study examined the effects of an adenine-type cytokinin 6-benzylaminopurine (6-BA) on the growth and metabolism characteristics of Botryococcus braunii, one of the most promising oil-rich algae for biofuel production. The results showed that 6-BA of low dose (0.1-1.0 mg L-1) would enhance the algal growth rate and biochemical synthesis, whereas too much (5.0 mg L-1) would be lethally toxic for B. braunii. Noticingly, though the maximum algal growth rate, chlorophyll and β-carotenoid content were observed in the treatment with 0.5 and/or 1.0 mg L-1 6-BA, both the maximum algal hydrocarbon content and the highest hydrocarbon productivity were observed in the treatment with 0.1 mg L-1 6-BA, which were respectively 2.45 and 3.48 times of the control (39.1% vs. 16.0%, 546 mg L-1 vs. 157 mg L-1). This finding has great implications for improving algae biofuels production by phytohormone.

scholarly journals Development of a screening procedure for the characterization of Botryococcus braunii strains for biofuel application

2016 ◽  
Vol 51 (11) ◽  
pp. 1855-1865 ◽  
Author(s):  
Benjamin Moutel ◽  
Olivier Gonçalves ◽  
Fabienne Le Grand ◽  
Marc Long ◽  
Philippe Soudant ◽  
...  
Keyword(s):  
Botryococcus Braunii ◽  
Screening Procedure

scholarly journals Similar morphological and chemical variations of Gloeocapsomorpha prisca in Ordovician sediments and cultured Botryococcus braunii as a response to changes in salinity

1992 ◽  
Vol 19 (4-6) ◽  
pp. 299-313 ◽  
Author(s):  
S. Derenne ◽  
P. Metzger ◽  
C. Largeau ◽  
P.F. Van Bergen ◽  
J.P. Gatellier ◽  
...  
Keyword(s):  
Botryococcus Braunii

Outdoor pilot-scale production of Botryococcus braunii in panel reactors

2012 ◽  
Vol 24 (6) ◽  
pp. 1353-1360 ◽  
Author(s):  
Jazmin Bazaes ◽  
Claudia Sepulveda ◽  
F. Gabriel Acién ◽  
Juan Morales ◽  
Leonel Gonzales ◽  
...  
Keyword(s):  
Botryococcus Braunii ◽  
Pilot Scale ◽  
Scale Production ◽  
Pilot Scale Production

scholarly journals Release of single cells from the colonial oil-producing alga Botryococcus braunii by chemical treatments

PROTOPLASMA ◽  
2013 ◽  
Vol 251 (1) ◽  
pp. 191-199 ◽  
Author(s):  
Liyuan Hou ◽  
Hyunsun Park ◽  
Shigeru Okada ◽  
Takeshi Ohama
Keyword(s):  
Single Cells ◽  
Botryococcus Braunii ◽  
Chemical Treatments
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