The Normal Salivary Gland Aspirate

2008 ◽  
pp. 41-55
Keyword(s):  
Salivary Gland ◽  
Normal Salivary Gland

Expression of TdT in Myoepithelial Cells: Investigation in Breasts, Sweat Glands, and Salivary Lesions Emphasizing the Never-Documented Immunohistochemical Findings

2020 ◽  
Vol 28 (7) ◽  
pp. 711-720
Author(s):  
Jun Zhou ◽  
Haimin Xu ◽  
Hong Zeng ◽  
Hongjun Ma ◽  
Jingjing Yu ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Myoepithelial Cells ◽  
Cross Reactivity ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Sweat Glands ◽  
Routine Practice ◽  
Cell Adenoma ◽  
Normal Salivary Gland ◽  
Hidradenoma Papilliferum ◽  
Myoepithelial Differentiation

Background. The expression of terminal deoxynucleotidyl transferase (TdT) in myoepithelial cells (MECs) within the breast was recently incidentally observed in our routine practice. This study aimed to elucidate the expression of TdT in MECs. Methods. TdT immunostaining was performed on 180 mammary, 89 cutaneous, and 94 salivary tissues or lesions. Other myoepithelial markers, including P63, calponin, and SMA as well as double staining for TdT and calponin, were also evaluated in some cases. Selected lesions with basal or myoid differentiation were also included in the investigation. Results. MECs were positive for TdT in mammary lesions that contained MECs (132/135) but negative when they did not contain MECs (45/45). MECs in sweat glands (24/30) and their neoplastic counterparts, including those in hidradenoma papilliferum (2/9), spiradenoma (6/6), and cutaneous mixed tumor (9/9), showed weak to moderate TdT positivity. MECs were variably immunolabeled for TdT in salivary or salivary gland–type tumors with myoepithelial differentiation (pleomorphic adenoma, 24/25; basal cell adenoma, 6/7; adenoid cystic carcinoma, 7/7; Warthin tumor, 0/6; mucoepidermoid carcinoma, 0/8; acinic cell carcinoma, 0/4), but MECs in normal salivary gland barely stained for TdT (30/32). Conclusions. Our findings indicate that TdT may be eligible as an additional auxiliary immunohistochemical marker as P63, but not a surrogate, to identify the MECs in the breast with limited cross-reactivity, particularly in lesions with a prominent proportion of MECs. Positivity for TdT, along with other relevant markers, in a subset of sweat gland lesions and salivary tumors may contribute to their diagnosis.

scholarly journals Histochemical Observations on Aminopeptidase Activity in Normal Salivary Gland

1960 ◽  
Vol 19 (3) ◽  
pp. 509-517 ◽  
Author(s):  
Kensaku KAWAKATSU ◽  
Masahiko MORI ◽  
Tsuneo MIZUSHIMA ◽  
Masaru MURAKAMI
Keyword(s):  
Salivary Gland ◽  
Aminopeptidase Activity ◽  
Normal Salivary Gland

The evaluation of salivary gland tumours using proliferating cell nuclear antigen

1997 ◽  
Vol 111 (6) ◽  
pp. 551-555 ◽  
Author(s):  
N. J. Trendell-Smith ◽  
J. Oates ◽  
J. Crocker
Keyword(s):  
Salivary Gland ◽  
Proliferating Cell Nuclear Antigen ◽  
Prognostic Value ◽  
Positive Control ◽  
Negative Control ◽  
Anaplastic Carcinoma ◽  
Nuclear Antigen ◽  
Salivary Gland Tumours ◽  
Normal Salivary Gland ◽  
Proliferating Cell

AbstractIn order to assess its discriminating and prognostic value, we studied the immunoreactivity for proliferating cell nuclear antigen (PCNA) in tissue from 52 human salivary gland tumours using the murine monoclonal antibody PC10.The PCNA percentage count, namely, the average number of positive nuclei counted per 100 randomly selected tumour cells was recorded for each tumour. Anaplastic carcinoma was used as a positive control and histologically ‘normal’ salivary gland and tonsil served as a negative control.A PCNA count of 30 per cent was postulated to predict malignancy within a given salivary gland tumour i.e. a PCNA count of 30 per cent or above would indicate malignant potential. This gave a sensitivity of 96.9 per cent and a specificity of 95.2 per cent and a positive predictive value of determining malignancy of 96.8 per cent.We conclude that PCNA immunoreactivity is useful in discriminating between benign and malignant salivary gland tumours and that it may have prognostic value in this diverse group of neoplasms.

Constitutive (HO-2) and inducible (HO-1) haem oxygenase in pleomorphic adenomas of the human parotid: an immunocytochemical study

2005 ◽  
Vol 119 (3) ◽  
pp. 179-183 ◽  
Author(s):  
Stephen Lo ◽  
Silvana Di Palma ◽  
Hafsa Yusuf ◽  
Andrew W McCombe
Keyword(s):  
Blood Flow ◽  
Salivary Gland ◽  
Heat Shock ◽  
Tumour Cells ◽  
Positive Staining ◽  
Immunocytochemical Study ◽  
Pleomorphic Adenomas ◽  
Normal Salivary Gland ◽  
Ductal Differentiation ◽  
Haem Oxygenase

This study examines the expression HO-1 and HO-2 isozymes in human parotid pleomorphic adenomas. They are members of the heat shock protein family, and are thought to play a role in the regulation of tumoral blood flow. Immunocytochemistry using antibodies specific for HO-1 and HO-2 were undertaken in 12 pleomorphic adenoma specimens, all sections of which contained adjacent normal salivary tissue. Normal salivary gland acini and ducts displayed significantly stronger immunoreactivity for HO-2 compared to tumour cells (p < 0.001). Expression for HO-1 was minimal in both normal salivary gland acini and tumour cells with no difference (p = 1.000). However, positive staining for HO-1 was seen in normal salivary ducts and in pleomorphic adenomas showing ductal differentiation. In conclusion, this is the first study to examine the expression of HO-1 and HO-2 within normal salivary glands and pleomorphic adenomas. Our findings suggest that HO may be implicated in the pathogenesis of salivary pleomorphic adenomas.

scholarly journals Cancer Secretome May Influence BSP and DSP Expression in Human Salivary Gland Cells

2016 ◽  
Vol 65 (3) ◽  
pp. 139-151 ◽  
Author(s):  
Samantha Lynn Hamilton ◽  
Blake Ferando ◽  
Asha Sarah Eapen ◽  
Jennifer Chian Yu ◽  
Anita Rose Joy
Keyword(s):  
Salivary Gland ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Migration And Invasion ◽  
Salivary Gland Cancer ◽  
Cancer Tissue ◽  
Human Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Normal Salivary Gland

One of the biggest challenges in managing head and neck cancers, especially salivary gland cancers, is the identification of secreted biomarkers of the disease that can be evaluated noninvasively. A relevant source of enriched tumor markers could potentially be found in the tumor secretome. Although numerous studies have evaluated secretomes from various cancers, the influence of the cancer secretome derived from salivary gland cancers on the behavior of normal cells has not yet been elucidated. Our data indicate that secretome derived from salivary gland cancer cells can influence the expression of two potential biomarkers of oral cancer—namely, bone sialoprotein (BSP) and dentin sialoprotein (DSP)—in normal salivary gland cells. Using routine immunohistochemistry, immunofluorescence, and immunoblotting techniques, we demonstrate an enrichment of BSP and DSP in human salivary gland (HSG) cancer tissue, unique localizations of BSP and DSP in HSG cancer cells, and enriched expression of BSP and DSP in normal salivary gland cells exposed to a cancer secretome. The secretome domain of the cancer microenvironment could alter signaling cascades responsible for normal cell proliferation, migration, and invasion, thus enhancing cancer cell survival and the potential for cancer progression. The cancer secretome may be critical in maintaining and stimulating “cancer-ness,” thus potentially promoting specific hallmarks of metastasis.

Salivary gland choristoma of the middle ear: case treated with KTP laser

2000 ◽  
Vol 114 (7) ◽  
pp. 528-532 ◽  
Author(s):  
Pakpoom Supiyaphun ◽  
Kornkiat Snidvongs ◽  
Shanob Shuangshoti
Keyword(s):  
Salivary Gland ◽  
Facial Nerve ◽  
Middle Ear ◽  
Proximal Part ◽  
Ossicular Chain ◽  
Branchial Arch ◽  
Surgical Removal ◽  
Ktp Laser ◽  
Surgical Biopsy ◽  
Normal Salivary Gland

Salivary gland choristoma of the middle ear is rare. It consists of non-malignant, non-growing, normal salivary gland tissue in the middle ear. It is a developmental abnormality that occurs around the proximal part of the second branchial arch before the fourth month of intrauterine life.The authors found the 25th recorded case in our centre and another 24 reported cases from a review of the literature between 1961 and 1999. Intratympanic salivary gland choristoma frequently occurs during the first and second decades of life and with a female preponderance (56 per cent). Nearly all the patients (96 per cent) in our review presented with a hearing loss, that had begun since birth, in infancy, or during childhood. Tinnitus (28 per cent), and serous otitis media (24 per cent) were also commonly present. One case complained of otorrhoea. Intratympanic and extratympanic anomalies were found in 96.2 per cent and 34.6 per cent of cases respectively. Of these anomalies, ossicular chain (88.5 per cent), facial nerve (65.4 per cent), middle-ear muscles (30.8 per cent) and labyrinthine windows (23 per cent) were the four most common sites. Therefore, salivary gland choristoma may represent a manifestation of a congenital ear anomaly.Diagnosis of salivary gland choristoma is generally not documented pre-operatively, but is based on surgical biopsy and histopathological investigations. Treatment of this rare lesion depends on the size, location and extent of the mass, degree of anatomical abnormality and expertise of the surgeon. In difficult cases where the mass is attached to the dehiscent or inferiorly placed facial nerve, only biopsy is recommended. However, complete surgical removal is advocated for a mass that is easy to remove. KTP laser use via a 200 micron fibreoptic light carrier can facilitate removal especially in cases with ossicular chain involvement.

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