Mechanical Unfolding and Refolding of Single Membrane Proteins by Atomic Force Microscopy

2020 ◽  
pp. 359-372
Author(s):  
Noah Ritzmann ◽  
Johannes Thoma
Keyword(s):  
Atomic Force Microscopy ◽  
Membrane Proteins ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Membrane

Probing Single Membrane Proteins by Atomic Force Microscopy

2009 ◽  
pp. 449-485
Author(s):  
S. Scheuring ◽  
K. Tanuj Sapra ◽  
Daniel J. Müller
Keyword(s):  
Atomic Force Microscopy ◽  
Membrane Proteins ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Membrane

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

scholarly journals Molecular imaging of membrane proteins and microfilaments using atomic force microscopy

2010 ◽  
Vol 42 (9) ◽  
pp. 597 ◽  
Author(s):  
Se-Hui Jung ◽  
Donghyun Park ◽  
Jae Hyo Park ◽  
Young-Myeong Kim ◽  
Kwon-Soo Ha
Keyword(s):  
Atomic Force Microscopy ◽  
Molecular Imaging ◽  
Membrane Proteins ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals Practical applications of atomic force microscopy in biomedicine

STEMedicine ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. e15
Author(s):  
Nicola Galvanetto
Keyword(s):  
Atomic Force Microscopy ◽  
Drug Discovery ◽  
Membrane Proteins ◽  
Biological Samples ◽  
Live Imaging ◽  
Proof Of Concept ◽  
Living Matter ◽  
Practical Applications ◽  
Force Microscopy ◽  
Atomic Force

The last thirty years of progress of atomic force microscopy (AFM) applied to living matter is reviewed with a focus on potential uses in drug discovery or screening of patient samples. AFM-based technologies are still at Proof of Concept level - or below, however, they are particularly promising for i) live imaging of unlabeled membrane proteins and ii) nanomechanical screening of biological samples, e.g. cancer biopsies.

scholarly journals Characterization of the motion of membrane proteins using high-speed atomic force microscopy

2012 ◽  
Vol 7 (8) ◽  
pp. 525-529 ◽  
Author(s):  
Ignacio Casuso ◽  
Jonathan Khao ◽  
Mohamed Chami ◽  
Perrine Paul-Gilloteaux ◽  
Mohamed Husain ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Membrane Proteins ◽  
High Speed ◽  
Force Microscopy ◽  
Atomic Force
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