scholarly journals Practical applications of atomic force microscopy in biomedicine

STEMedicine ◽  
2020 ◽  
Vol 1 (2) ◽  
pp. e15
Author(s):  
Nicola Galvanetto
Keyword(s):  
Atomic Force Microscopy ◽  
Drug Discovery ◽  
Membrane Proteins ◽  
Biological Samples ◽  
Live Imaging ◽  
Proof Of Concept ◽  
Living Matter ◽  
Practical Applications ◽  
Force Microscopy ◽  
Atomic Force

The last thirty years of progress of atomic force microscopy (AFM) applied to living matter is reviewed with a focus on potential uses in drug discovery or screening of patient samples. AFM-based technologies are still at Proof of Concept level - or below, however, they are particularly promising for i) live imaging of unlabeled membrane proteins and ii) nanomechanical screening of biological samples, e.g. cancer biopsies.

Imaging of Xenopus laevis Oocyte Plasma Membrane in Physiological-Like Conditions by Atomic Force Microscopy

2013 ◽  
Vol 19 (5) ◽  
pp. 1358-1363 ◽  
Author(s):  
Massimo Santacroce ◽  
Federica Daniele ◽  
Andrea Cremona ◽  
Diletta Scaccabarozzi ◽  
Michela Castagna ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Resolution ◽  
Membrane Proteins ◽  
Xenopus Laevis ◽  
Functional Study ◽  
Xenopus Laevis Oocyte ◽  
Force Microscopy ◽  
Atomic Force ◽  
Afm Imaging

AbstractXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X. laevis oocytes. In particular, we developed and optimized a new sample preparation protocol, based on the purification of plasma membranes by ultracentrifugation on a sucrose gradient, to perform a high-resolution AFM imaging of X. laevis oocyte plasma membrane in physiological-like conditions. Reproducible AFM topographs allowed visualization and dimensional characterization of membrane patches, whose height corresponds to a single lipid bilayer, as well as the presence of nanometer structures embedded in the plasma membrane and identified as native membrane proteins. The described method appears to be an applicable tool for performing high-resolution AFM imaging of X. laevis oocyte plasma membrane in a physiological-like environment, thus opening promising perspectives for studying in situ cloned membrane proteins of relevant biomedical/pharmacological interest expressed in this biological system.

scholarly journals 3pM_K3Non-invasive Live-imaging of Plasma Membrane and Cortical Actin Dynamics in Endocytic Process by High-speed Atomic Force Microscopy

Microscopy ◽  
2018 ◽  
Vol 67 (suppl_2) ◽  
pp. i10-i10
Author(s):  
Aiko Yoshida ◽  
Yoshitsuna Itagaki ◽  
Yuki Suzuki ◽  
Shige H. Yoshimura
Keyword(s):  
Atomic Force Microscopy ◽  
Plasma Membrane ◽  
High Speed ◽  
Live Imaging ◽  
Actin Dynamics ◽  
Cortical Actin ◽  
Force Microscopy ◽  
Atomic Force

Quantitative mechanical analysis of indentations on layered, soft elastic materials

Soft Matter ◽  
2019 ◽  
Vol 15 (8) ◽  
pp. 1776-1784 ◽  
Author(s):  
Bryant L. Doss ◽  
Kiarash Rahmani Eliato ◽  
Keng-hui Lin ◽  
Robert Ros
Keyword(s):  
Atomic Force Microscopy ◽  
Elastic Modulus ◽  
Cell Mechanics ◽  
Biological Samples ◽  
Mechanical Analysis ◽  
Elastic Materials ◽  
Mechanical Heterogeneity ◽  
Popular Method ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy (AFM) is becoming an increasingly popular method for studying cell mechanics, however the existing analysis tools for determining the elastic modulus from indentation experiments are unable to quantitatively account for mechanical heterogeneity commonly found in biological samples.

Probing Single Membrane Proteins by Atomic Force Microscopy

2009 ◽  
pp. 449-485
Author(s):  
S. Scheuring ◽  
K. Tanuj Sapra ◽  
Daniel J. Müller
Keyword(s):  
Atomic Force Microscopy ◽  
Membrane Proteins ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Membrane

scholarly journals Molecular imaging of membrane proteins and microfilaments using atomic force microscopy

2010 ◽  
Vol 42 (9) ◽  
pp. 597 ◽  
Author(s):  
Se-Hui Jung ◽  
Donghyun Park ◽  
Jae Hyo Park ◽  
Young-Myeong Kim ◽  
Kwon-Soo Ha
Keyword(s):  
Atomic Force Microscopy ◽  
Molecular Imaging ◽  
Membrane Proteins ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy contact, tapping, and jumping modes for imaging biological samples in liquids

2004 ◽  
Vol 69 (3) ◽  
Author(s):  
F. Moreno-Herrero ◽  
J. Colchero ◽  
J. Gómez-Herrero ◽  
A. M. Baró
Keyword(s):  
Atomic Force Microscopy ◽  
Biological Samples ◽  
Force Microscopy ◽  
Atomic Force

Tapping, pulling, probing: atomic force microscopy in drug discovery

2004 ◽  
Vol 1 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Justin Legleiter ◽  
Tomasz Kowalewski
Keyword(s):  
Atomic Force Microscopy ◽  
Drug Discovery ◽  
Force Microscopy ◽  
Atomic Force
Sign in / Sign up

Export Citation Format

Share Document