Optogenetics and Optical Tools in Automated Patch Clamping

2020 ◽  
pp. 311-330
Author(s):  
Kim Boddum ◽  
Peder Skafte-Pedersen ◽  
Jean-Francois Rolland ◽  
Sandra Wilson
Keyword(s):  
Patch Clamping ◽  
Optical Tools

Novel processing for a polymer patch clamping system

2009 ◽  
Author(s):  
S. Wilson ◽  
W. Pfleging ◽  
M. Bruns ◽  
A. Welle ◽  
P.B. Kirby
Keyword(s):  
Patch Clamping ◽  
Clamping System ◽  
Novel Processing

scholarly journals Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts

2008 ◽  
Vol 2008 (6) ◽  
pp. pdb.prot5014-pdb.prot5014 ◽  
Author(s):  
W. Zhang ◽  
S. E. Nilson ◽  
S. M. Assmann
Keyword(s):  
Guard Cell ◽  
Patch Clamping ◽  
Whole Cell ◽  
Guard Cell Protoplasts

The photonics project web based electro-optical tools

2021 ◽  
Author(s):  
John W. Devitt ◽  
Ron Driggers ◽  
Anjali Singh ◽  
Orges Furxhi ◽  
Mel Friedman ◽  
...  
Keyword(s):  
Web Based ◽  
Optical Tools

Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording

Lab on a Chip ◽  
2009 ◽  
Vol 9 (16) ◽  
pp. 2370 ◽  
Author(s):  
Chang-Yu Chen ◽  
Ting-Yuan Tu ◽  
Chang-Hung Chen ◽  
De-Shien Jong ◽  
Andrew M. Wo
Keyword(s):  
Ion Channel ◽  
High Yield ◽  
Patch Clamping

scholarly journals Optical tools for ocean monitoring and research

Ocean Science ◽  
2009 ◽  
Vol 5 (4) ◽  
pp. 661-684 ◽  
Author(s):  
C. Moore ◽  
A. Barnard ◽  
P. Fietzek ◽  
M. R. Lewis ◽  
H. M. Sosik ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Ocean Color ◽  
Analytical Techniques ◽  
Optical Measurements ◽  
Physical Parameters ◽  
Optical Tools ◽  
Wide Range ◽  
Radiance Distribution ◽  
Ocean Monitoring ◽  
Methane Carbon

Abstract. Requirements for understanding the relationships between ocean color and suspended and dissolved materials within the water column, and a rapidly emerging photonics and materials technology base for performing optical based analytical techniques have generated a diverse offering of commercial sensors and research prototypes that perform optical measurements in water. Through inversion, these tools are now being used to determine a diverse set of related biogeochemical and physical parameters. Techniques engaged include measurement of the solar radiance distribution, absorption, scattering, stimulated fluorescence, flow cytometry, and various spectroscopy methods. Selective membranes and other techniques for material isolation further enhance specificity, leading to sensors for measurement of dissolved oxygen, methane, carbon dioxide, common nutrients and a variety of other parameters. Scientists are using these measurements to infer information related to an increasing set of parameters and wide range of applications over relevant scales in space and time.

Voltage and patch clamping with microelectrodes

1985 ◽  
Vol 10 (12) ◽  
pp. 494
Author(s):  
Julian I. Schroeder
Keyword(s):  
Patch Clamping

scholarly journals Evaluation of the role of cholinergic and nitrergic myenteric neurons in colonic motility using genetically‐encoded optical tools

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Thomas W. Gould ◽  
Camilla Fedi ◽  
Claire DeAngeli ◽  
Dante J. Heredia
Keyword(s):  
Colonic Motility ◽  
Myenteric Neurons ◽  
Optical Tools

scholarly journals Toxoplasma gondii injected neurons localize to the cortex and striatum and have altered firing

2021 ◽  
Author(s):  
Oscar A. Mendez ◽  
Emiliano Flores Machado ◽  
Jing Lu ◽  
Anita A. Koshy
Keyword(s):  
Toxoplasma Gondii ◽  
Single Neuron ◽  
Latent Infection ◽  
Ex Vivo ◽  
Medium Spiny Neurons ◽  
Patch Clamping ◽  
Spiny Neurons ◽  
The Brain ◽  
Mapping Program

AbstractToxoplasma gondii is an intracellular parasite that causes a long-term latent infection of neurons. Using a custom MATLAB-based mapping program in combination with a mouse model that allows us to permanently mark neurons injected with parasite proteins, we found that Toxoplasma-injected neurons (TINs) are heterogeneously distributed in the brain, primarily localizing to the cortex followed by the striatum. Using immunofluorescence co-localization assays, we determined that cortical TINs are commonly (>50%) excitatory neurons (FoxP2+) and that striatal TINs are often (>65%) medium spiny neurons (MSNs) (FoxP2+). As MSNs have highly characterized electrophysiology, we used ex vivo slices from infected mice to perform single neuron patch-clamping on striatal TINs and neighboring uninfected MSNs (bystander MSNs). These studies demonstrated that TINs have highly abnormal electrophysiology, while the electrophysiology of bystander MSNs was akin to that of MSNs from uninfected mice. Collectively, these data offer new neuroanatomic and electrophysiologic insights into CNS toxoplasmosis.

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