Measuring During Proliferative Cell

2020 ◽  
pp. 19-42
Author(s):  
Claudia Hinze ◽  
Kieran McGourty ◽  
Emmanuel Boucrot
Keyword(s):  
Proliferative Cell

scholarly journals Phloretin Ameliorates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by Regulating the Inflammatory Response, Oxidative Stress and Apoptosis

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 743
Author(s):  
Chao Yu Hsu ◽  
Yi Sheng Lin ◽  
Wei Chun Weng ◽  
Lauren Panny ◽  
Hsiang Lai Chen ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Weight Ratio ◽  
Nuclear Antigen ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Body Weight Ratio ◽  
Proliferative Cell Nuclear Antigen ◽  
Prostate Weight ◽  
Prostatic Enlargement ◽  
Inflammatory Proteins ◽  
Proliferative Cell

The inflammatory process is proposed to be one of the factors to benign prostatic enlargement (BPH), and this is the first study examining the anti-inflammatory ability of phloretin in treating rats with testosterone-induced BPH. BPH would be induced by testosterone (10 mg/kg/day testosterone subcutaneously for 28 days), and the other groups of rats were treated with phloretin 50 mg/kg/day or 100 mg/kg/day orally (phr50 or phr100 group) after induction. Prostate weight and prostate weight to body weight ratio were significantly reduced in the Phr100 group. Reduced dihydrotestosterone without interfering with 5α-reductase was observed in the phr100 group. In inflammatory proteins, reduced IL-6, IL-8, IL-17, NF-κB, and COX-2 were seen in the phr100 group. In reactive oxygen species, malondialdehyde was reduced, and superoxide dismutase and glutathione peroxidase were elevated in the phr100 group. In apoptotic assessment, elevated cleaved caspase-3 was observed in rats of the phr100 group. Enhanced pro-apoptotic Bax and reduced anti-apoptotic Bc1-2 could be seen in the phr100 group. In histological stains, markedly decreased glandular hyperplasia and proliferative cell nuclear antigen were observed with reduced expression in the phr100 group. Meanwhile, positive cells of terminal deoxynucleotidyl transferase dUTP nick end labeling were increased in the phr100 group. In conclusion, the treatment of phloretin 100 mg/kg/day could ameliorate testosterone-induced BPH.

The Proliferative Cell Fraction in Cytology Specimens: A Study of Human Esophageal Carcinoma

1992 ◽  
Vol 98 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Hironobu Sasano ◽  
Shukichi Miyazaki ◽  
Tetsurou Nishihira ◽  
Takashi Sawai ◽  
Hiroshi Nagura
Keyword(s):  
Esophageal Carcinoma ◽  
Cell Fraction ◽  
Proliferative Cell

Proton magnetic resonance spectroscopy in pituitary macroadenomas: preliminary results

2008 ◽  
Vol 109 (2) ◽  
pp. 306-312 ◽  
Author(s):  
Andreas Stadlbauer ◽  
Michael Buchfelder ◽  
Christopher Nimsky ◽  
Wolfgang Saeger ◽  
Erich Salomonowitz ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Mr Spectroscopy ◽  
Spectroscopy Data ◽  
Cell Index ◽  
Volume Of Interest ◽  
Metabolite Concentrations ◽  
The Magnetic Field ◽  
Proliferative Cell ◽  
Mib 1 ◽  
Pituitary Macroadenomas

Object The aim of this study was to correlate proton MR (1H-MR) spectroscopy data with histopathological and surgical findings of proliferation and hemorrhage in pituitary macroadenomas. Methods Quantitative 1H-MR spectroscopy was performed on a 1.5-T unit in 37 patients with pituitary macroadenomas. A point-resolved spectroscopy sequence (TR 2000 msec, TE 135 msec) with 128 averages and chemical shift selective pulses for water suppression was used. Voxel dimensions were adapted to ensure that the volume of interest was fully located within the lesion and to obtain optimal homogeneity of the magnetic field. In addition, water-unsuppressed spectra (16 averages) were acquired from the same volume of interest for eddy current correction, absolute quantification of metabolite signals, and determination of full width at half maximum of the unsuppressed water peak (FWHMwater). Metabolite concentrations of choline-containing compounds (Cho) were computed using the LCModel program and correlated with MIB-1 as a proliferative cell index from a tissue specimen. Results In 16 patients harboring macroadenomas without hemorrhage, there was a strong positive linear correlation between metabolite concentrations of Cho and the MIB-1 proliferative cell index (R = 0.819, p < 0.001). The metabolite concentrations of Cho ranged from 1.8 to 5.2 mM, and the FWHMwater was 4.4–11.7 Hz. Eleven patients had a hemorrhagic adenoma and showed no assignable metabolite concentration of Cho, and the FWHMwater was 13.4–24.4 Hz. In 10 patients the size of the lesion was too small (< 20 mm in 2 directions) for the acquisition of MR spectroscopy data. Conclusions Quantitative 1H-MR spectroscopy provided important information on the proliferative potential and hemorrhaging of pituitary macroadenomas. These data may be useful for noninvasive structural monitoring of pituitary macroadenomas. Differences in the FWHMwater could be explained by iron ions of hemosiderin, which lead to worsened homogeneity of the magnetic field.

scholarly journals Cardiorespiratory disorders in preschool aged children associated with aerogenic impact of benzene, phenol and formaldehyde

2019 ◽  
Vol 95 (1) ◽  
pp. 70-74
Author(s):  
Olga A. Maklakova ◽  
S. L. Valina
Keyword(s):  
Qt Interval ◽  
Respiratory Diseases ◽  
Creatine Phosphokinase ◽  
Reference Group ◽  
Cell Activity ◽  
Glutathione S Transferase ◽  
Chronic Respiratory Diseases ◽  
School Aged Children ◽  
Proliferative Cell ◽  
Airway Conductance

There was performed an examination of 437 pre-school aged children living in the conditions of the pollution of atmospheric air with benzene, phenol andformaldehyde. Children with the elevated blood content of benzene, phenol andformaldehyde were detected to be diagnosed as having chronic respiratory diseases and asteno-neurotic syndrome by 3 and 1.3 times more often, respectively, in contrast to the reference group. Cardiorespiratory disorders in children with the elevated content of benzene, phenol and formaldehyde are manifested by restrictive and mixed disorders of airway conductance followed by the increase in markers of proliferative cell activity (CA-72-4 and M 20), changes in the electrophysiologicalprocesses in cardiac muscle pronounced by the decreased electric ventricular systole (QT interval) followed by the decline of the content in blood of glutathione-S-transferase, zAMF, nitrogen oxide and the increase in the lipids hydroperoxide level, and creatine phosphokinase activity.

High-density induction of a quiescent cell state in Physarum polycephalum

1977 ◽  
Vol 25 (1) ◽  
pp. 179-190
Author(s):  
L.E. McAlister ◽  
V.F. Allison ◽  
J.R. Jeter ◽  
C. Nations
Keyword(s):  
Physarum Polycephalum ◽  
Rna Synthesis ◽  
High Density ◽  
Mitochondrial Morphology ◽  
Nuclear Actin ◽  
Active Growth ◽  
Cell State ◽  
Cytoplasmic Organization ◽  
Protein Complement ◽  
Proliferative Cell

The non-histone protein complement of Physarum polycephalum changes rapidly when microplasmodia are subjected to conditions of high density. The changes in these proteins induced by high density are similar to the changes observed during starvation-induced encystment. A 50% decrease in DNA synthesis, observed after 7 h of starvation, is observed after only 1 h of high density. High density also results in a decrease in RNA synthesis comparable to decreases induced by prolonged starvation. Total heterochromatin increases in response to either high density or starvation. Increased heterochromatization is preceded by an increase in nuclear actin. Mitochondrial morphology and cytoplasmic organization are also similarly altered by starvation and high density. These observations suggest the possibility of a generalized mechanism for cellular transition from active growth to a non-proliferative cell state.

scholarly journals A low-temperature method for the isolation of small-intestinal epithelium along the crypt-villus axis

1991 ◽  
Vol 280 (2) ◽  
pp. 331-334 ◽  
Author(s):  
N Flint ◽  
F L Cove ◽  
G S Evans
Keyword(s):  
Small Intestine ◽  
Low Temperature ◽  
Intestinal Epithelium ◽  
Sequential Fractionation ◽  
Potential Value ◽  
Temperature Method ◽  
Small Intestinal ◽  
The Comparative Study ◽  
Cell Phenotypes ◽  
Proliferative Cell

A variety of enzymic and non-enzymic methods to isolate epithelium from the small intestine have been previously published. Sequential fractionation of cells from the villus to the crypt has been reported in some of these papers, which allows the comparative study of terminally differentiated and proliferative cell phenotypes. However, these methods often involve the incubation of tissues at 37 degrees C, which may affect the structural and biochemical integrity of the cells. We have developed a rapid low-temperature (4 degrees C) method for isolating purified populations of crypt and villus cells from mouse and rat intestines. The fractionated cells have been partially characterized, and the potential value of the procedure has been indicated by the ability to analyse the comparative protein and mRNA expression along the crypt-villus axis.

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