Recombinant Expression and Purification of Cannabinoid Receptor CB2, a G Protein-Coupled Receptor

2021 ◽  
pp. 61-76
Author(s):  
Alexei A. Yeliseev
Keyword(s):  
G Protein ◽  
Cannabinoid Receptor ◽  
Recombinant Expression ◽  
Expression And Purification ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals G protein-coupled receptor GPR55 promotes colorectal cancer and has opposing effects to cannabinoid receptor 1

2017 ◽  
Vol 142 (1) ◽  
pp. 121-132 ◽  
Author(s):  
Carina Hasenoehrl ◽  
David Feuersinger ◽  
Eva M Sturm ◽  
Thomas Bärnthaler ◽  
Ellen Heitzer ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
G Protein ◽  
Cannabinoid Receptor ◽  
G Protein Coupled Receptor ◽  
Cannabinoid Receptor 1 ◽  
Opposing Effects ◽  
G Protein Coupled

scholarly journals Interactions of the G protein-coupled receptor-associated sorting proteins (GASP) 1 and 2 with the novel cannabinoid receptor GPR55

2008 ◽  
Vol 8 (Suppl 1) ◽  
pp. A16
Author(s):  
Julia Kargl ◽  
Nariman Balenga ◽  
Lene Martini ◽  
Jennifer Whistler ◽  
Maria Waldhoer
Keyword(s):  
G Protein ◽  
Cannabinoid Receptor ◽  
The Novel ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals SGIP1 modulates kinetics and interactions of the cannabinoid receptor 1 and G protein‐coupled receptor kinase 3 signalosome

2021 ◽  
Author(s):  
Matej Gazdarica ◽  
Judith Noda ◽  
Oleh Durydivka ◽  
Vendula Novosadova ◽  
Ken Mackie ◽  
...  
Keyword(s):  
G Protein ◽  
Cannabinoid Receptor ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
Cannabinoid Receptor 1 ◽  
G Protein Coupled

Scotopic vision in the monkey is modulated by the G protein-coupled receptor 55

2016 ◽  
Vol 33 ◽  
Author(s):  
JOSEPH BOUSKILA ◽  
VANESSA HARRAR ◽  
PASHA JAVADI ◽  
CHRISTIAN CASANOVA ◽  
YOSHIO HIRABAYASHI ◽  
...  
Keyword(s):  
G Protein ◽  
Cannabinoid Receptor ◽  
Vervet Monkeys ◽  
G Protein Coupled Receptor ◽  
Full Field ◽  
Scotopic Vision ◽  
Cone System ◽  
Photopic Erg ◽  
Endogenous Cannabinoid ◽  
G Protein Coupled

AbstractThe endogenous cannabinoid system plays important roles in the retina of mice and monkeys via their classic CB1 and CB2 receptors. We have previously reported that the G protein-coupled receptor 55 (GPR55), a putative cannabinoid receptor, is exclusively expressed in rod photoreceptors in the monkey retina, suggesting its possible role in scotopic vision. To test this hypothesis, we recorded full-field electroretinograms (ERGs) after the intravitreal injection of the GPR55 agonist lysophosphatidylglucoside (LPG) or the selective GPR55 antagonist CID16020046 (CID), under light- and dark-adapted conditions. Thirteen vervet monkeys (Chlorocebus sabaeus) were used in this study: four controls (injected with the vehicle dimethyl sulfoxide, DMSO), four injected with LPG and five with CID. We analyzed amplitudes and latencies of the a-wave (photoreceptor responses) and the b-wave (rod and cone system responses) of the ERG. Our results showed that after injection of LPG, the amplitude of the scotopic b-wave was significantly higher, whereas after the injection of CID, it was significantly decreased, compared to the vehicle (DMSO). On the other hand, the a-wave amplitude, and the a-wave and b-wave latencies, of the scotopic ERG responses were not significantly affected by the injection of either compound. Furthermore, the photopic ERG waveforms were not affected by either drug. These results support the hypothesis that GPR55 plays an instrumental role in mediating scotopic vision.

scholarly journals Expression and Purification of G Protein-Coupled Receptor (GPCR) Transmembrane Peptides

2011 ◽  
Vol 100 (3) ◽  
pp. 381a
Author(s):  
Zachary T. Britton ◽  
Elizabeth I. Hanle ◽  
Anne S. Robinson
Keyword(s):  
G Protein ◽  
Expression And Purification ◽  
G Protein Coupled Receptor ◽  
Transmembrane Peptides ◽  
G Protein Coupled
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