Large-scale Expression and Purification of a G-protein-coupled Receptor for Structure Determination – An Overview

2005 ◽  
Vol 6 (2-3) ◽  
pp. 159-163 ◽  
Author(s):  
Reinhard Grisshammer ◽  
Jim F. White ◽  
Loc B. Trinh ◽  
Joseph Shiloach
Keyword(s):  
G Protein ◽  
Structure Determination ◽  
Large Scale ◽  
Expression And Purification ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

Large-scale molecular dynamics of a G protein-coupled receptor, the human 5-HT4 serotonin receptor, in a lipid bilayer

2007 ◽  
Vol 817 (1-3) ◽  
pp. 19-26 ◽  
Author(s):  
L. Rivail ◽  
C. Chipot ◽  
B. Maigret ◽  
I. Bestel ◽  
S. Sicsic ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
G Protein ◽  
Lipid Bilayer ◽  
Large Scale ◽  
Serotonin Receptor ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4

2009 ◽  
Vol 106 (29) ◽  
pp. 11925-11930 ◽  
Author(s):  
B. L. Cook ◽  
D. Steuerwald ◽  
L. Kaiser ◽  
J. Graveland-Bikker ◽  
M. Vanberghem ◽  
...  
Keyword(s):  
G Protein ◽  
Olfactory Receptor ◽  
Large Scale ◽  
Scale Production ◽  
G Protein Coupled Receptor ◽  
Large Scale Production ◽  
G Protein Coupled

scholarly journals Expression and Purification of G Protein-Coupled Receptor (GPCR) Transmembrane Peptides

2011 ◽  
Vol 100 (3) ◽  
pp. 381a
Author(s):  
Zachary T. Britton ◽  
Elizabeth I. Hanle ◽  
Anne S. Robinson
Keyword(s):  
G Protein ◽  
Expression And Purification ◽  
G Protein Coupled Receptor ◽  
Transmembrane Peptides ◽  
G Protein Coupled

scholarly journals Control of oviductal fluid flow by the G-protein coupled receptor Adgrd1 is essential for murine embryo transit

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Enrica Bianchi ◽  
Yi Sun ◽  
Alexandra Almansa-Ordonez ◽  
Michael Woods ◽  
David Goulding ◽  
...  
Keyword(s):  
Fluid Flow ◽  
G Protein ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Cumulus Cells ◽  
First Trimester ◽  
G Protein Coupled Receptor ◽  
Embryo Transport ◽  
Oviductal Fluid ◽  
G Protein Coupled

AbstractDysfunction of embryo transport causes ectopic pregnancy which affects approximately 2% of conceptions in the US and Europe, and is the most common cause of pregnancy-related death in the first trimester. Embryo transit involves a valve-like tubal-locking phenomenon that temporarily arrests oocytes at the ampullary-isthmic junction (AIJ) where fertilisation occurs, but the mechanisms involved are unknown. Here we show that female mice lacking the orphan adhesion G-protein coupled receptor Adgrd1 are sterile because they do not relieve the AIJ restraining mechanism, inappropriately retaining embryos within the oviduct. Adgrd1 is expressed on the oviductal epithelium and the post-ovulatory attenuation of tubal fluid flow is dysregulated in Adgrd1-deficient mice. Using a large-scale extracellular protein interaction screen, we identified Plxdc2 as an activating ligand for Adgrd1 displayed on cumulus cells. Our findings demonstrate that regulating oviductal fluid flow by Adgrd1 controls embryo transit and we present a model where embryo arrest at the AIJ is due to the balance of abovarial ciliary action and the force of adovarial tubal fluid flow, and in wild-type oviducts, fluid flow is gradually attenuated through Adgrd1 activation to enable embryo release. Our findings provide important insights into the molecular mechanisms involved in embryo transport in mice.

scholarly journals Automated large-scale purification of a G protein-coupled receptor for neurotensin

FEBS Letters ◽  
2004 ◽  
Vol 564 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Jim F White ◽  
Loc B Trinh ◽  
Joseph Shiloach ◽  
Reinhard Grisshammer
Keyword(s):  
G Protein ◽  
Large Scale ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals Expression and Purification of theSaccharomyces cerevisiaeα-Factor Receptor (Ste2p), a 7-Transmembrane-segment G Protein-coupled Receptor

1997 ◽  
Vol 272 (24) ◽  
pp. 15553-15561 ◽  
Author(s):  
Nathaniel E. David ◽  
Melanie Gee ◽  
Birgitte Andersen ◽  
Fred Naider ◽  
Jeremy Thorner ◽  
...  
Keyword(s):  
G Protein ◽  
Transmembrane Segment ◽  
Expression And Purification ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled
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