A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules

2021 ◽  
pp. 79-87
Author(s):  
Qiaojing Li ◽  
Hideaki Yoshimura ◽  
Takeaki Ozawa
Keyword(s):  
Cell Fusion ◽  
Bioactive Molecules ◽  
Split Luciferase

scholarly journals Using a split luciferase assay (SLA) to measure the kinetics of cell–cell fusion mediated by herpes simplex virus glycoproteins

Methods ◽  
2015 ◽  
Vol 90 ◽  
pp. 68-75 ◽  
Author(s):  
Wan Ting Saw ◽  
Zene Matsuda ◽  
Roselyn J. Eisenberg ◽  
Gary H. Cohen ◽  
Doina Atanasiu
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cell Fusion ◽  
Luciferase Assay ◽  
Simplex Virus ◽  
Kinetics Of ◽  
Split Luciferase ◽  
Cell Cell

A robust split-luciferase-based cell fusion screening for discovering myogenesis-promoting molecules

The Analyst ◽  
2018 ◽  
Vol 143 (14) ◽  
pp. 3472-3480 ◽  
Author(s):  
Qiaojing Li ◽  
Hideaki Yoshimura ◽  
Maki Komiya ◽  
Ken Tajiri ◽  
Motonari Uesugi ◽  
...  
Keyword(s):  
Cell Fusion ◽  
Split Luciferase

Screening by the luciferase complementation-based cell fusion assay discovered two myogenesis-promoting chemicals.

Bifluoride Ion Mediated SuFEx Trifluoromethylation of Sulfonyl Fluorides and Iminosulfur Oxydifluorides

2018 ◽  
Author(s):  
Christopher J. Smedley ◽  
Bing Gao ◽  
Suhua Li ◽  
Qinheng Zheng ◽  
Andrew Molino ◽  
...  
Keyword(s):  
Breast Cancer Cells ◽  
Catalytic Mechanism ◽  
Bioactive Molecules ◽  
Chromatographic Purification ◽  
Rapid Exchange ◽  
Nucleophilic Displacement ◽  
Sulfur Fluoride ◽  
Reactions With Nucleophiles ◽  
New Generation ◽  
Selection Of

Sulfur-Fluoride Exchange (SuFEx) is the new generation click chemistry transformation exploiting the unique properties of S-F bonds and their ability to undergo near-perfect reactions with nucleophiles. We report here the first SuFEx based protocol for the efficient synthesis of pharmaceutically important triflones and bis(trifluoromethyl)sulfur oxyimines from the corresponding sulfonyl fluorides and iminosulfur oxydifluorides, respectively. The new protocol involves the rapid exchange of the S-F bond with trifluoromethyltrimethylsilane (TMSCF<sub>3</sub>) upon activation with potassium bifluoride in anhydrous DMSO. The reaction tolerates a wide selection of substrates and proceeds under mild conditions without need for chromatographic purification. A tentative catalytic mechanism is proposed supported by DFT calculations, involving formation of the free trifluoromethyl anion followed by nucleophilic displacement of the S-F through a five-coordinate intermediate. The preparation of a benzothiazole derived bis(trifluoromethyl)sulfur oxyimine with cytotoxic selectivity for MCF7 breast cancer cells demonstrates the utility of this methodology for the late-stage functionalization of bioactive molecules.<br>

Reductive Cleavage of Secondary Sulfonamides: Converting Terminal Functional Groups into Versatile Synthetic Handles

2019 ◽  
Author(s):  
Patrick Fier ◽  
Suhong Kim ◽  
Kevin M. Maloney
Keyword(s):  
Functional Groups ◽  
Late Stage ◽  
Reductive Cleavage ◽  
Bioactive Molecules ◽  
General Method

Sulfonamides are pervasive in drugs and agrochemicals, yet are typically considered as terminal functional groups rather than synthetic handles. To enable the general late-stage functionalization of secondary sulfonamides, we have developed a mild and general method to reductively cleave the N-S bonds of sulfonamides to generate sulfinates and amines, components which can further react <i>in-situ</i> to access a variety of other medicinally relevant functional groups. The utility of this platform is highlighted by the selective manipulation of several complex bioactive molecules.

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