Screening of Actinobacteria for Anti-TB Activity by Microplate Alamar Blue Assay (MABA)

2022 ◽  
pp. 401-403
Author(s):  
Shuai Wei ◽  
Shucheng Liu ◽  
Ramachandran Chelliah ◽  
Deog-Hwan Oh
Keyword(s):  
Alamar Blue Assay ◽  
Alamar Blue

scholarly journals The Influence of Astaxanthin on the Proliferation of Adipose-derived Mesenchymal Stem Cells in Gelatin-Methacryloyl (GelMA) Hydrogels

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2416 ◽  
Author(s):  
Bo Young Choi ◽  
Elna Paul Chalisserry ◽  
Myoung Hwan Kim ◽  
Hyun Wook Kang ◽  
Il-Whan Choi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cellular Proliferation ◽  
Three Dimensional ◽  
Confocal Imaging ◽  
Alamar Blue Assay ◽  
Ratio Test ◽  
Alamar Blue ◽  
Chemical Structures

Recently, astaxanthin, a red lipophilic pigment belonging to the xanthophyllic family of carotenoids, has shown the feasibility of its uses in tissue engineering and regenerative medicine, due to its excellent antioxidant activities and its abilities to enhance the self-renewal potency of stem cells. In this study, we demonstrate the influence of astaxanthin on the proliferation of adipose-derived mesenchymal stem cells in tissue-engineered constructs. The tissue engineered scaffolds were fabricated using photopolymerizable gelatin methacryloyl (GelMA) with different concentrations of astaxanthin. The effects of astaxanthin on cellular proliferation in two-dimensional environments were assessed using alamar blue assay and reverse transcription polymerase chain reaction (RT-PCR). Then, rheological properties, chemical structures and the water absorption of the fabricated astaxanthin-incorporated GelMA hydrogels were characterized using NMR analysis, rheological analysis and a swelling ratio test. Finally, the influence in three-dimensional environments of astaxanthin-incorporated GelMA hydrogels on the proliferative potentials of adipose-derived stem cells was assessed using alamar blue assay and the confocal imaging with Live/dead staining. The experimental results of the study indicate that an addition of astaxanthin promises to induce stem cell potency via proliferation, and that it can be a useful tool for a three-dimensional culture system and various tissue engineering applications.

scholarly journals Synthesis, Spectral Characterization and Evaluation of Pharmacodynamic Activity of Copper and Nickel Complexes of Ethambutol Dihydrochloride

2006 ◽  
Vol 3 (4) ◽  
pp. 274-277 ◽  
Author(s):  
M. Mathrusri Annapurna ◽  
M. E. Bhanoji Rao ◽  
B. V. V. Ravi Kumar
Keyword(s):  
Metal Ion ◽  
Copper Complexes ◽  
Nickel Complexes ◽  
Octahedral Geometry ◽  
Spectral Characterization ◽  
Alamar Blue Assay ◽  
Alamar Blue ◽  
Thermal Data ◽  
Lowenstein Jensen ◽  
Assay Methods

Nickel and copper complexes of ethambutol [EB] were synthesized and characterized. On the basis of spectral and thermal data an octahedral geometry was assigned to the complexes with molecular formulae [Cu(C10H24N2O2)2Cl2] 5H2O and [Ni(C10H24N2O2)2Cl2] 2H2O. Coordination of ethylene diamine derivative to the metal ion was proposed. The anti-tubercular activity of the complexes was evaluated using Lowenstein–Jensen medium and MABA [Microplate Alamar Blue Assay] methods.

Assessment of the Alamar Blue assay for cellular growth and viability in vitro

1997 ◽  
Vol 204 (2) ◽  
pp. 205-208 ◽  
Author(s):  
Grace R Nakayama ◽  
Maureen C Caton ◽  
Michael P Nova ◽  
Zahra Parandoosh
Keyword(s):  
Cellular Growth ◽  
Alamar Blue Assay ◽  
Alamar Blue

Propidium iodide-based methods for monitoring drug action in the kinetoplastidae: Comparison with the Alamar Blue assay

2008 ◽  
Vol 382 (2) ◽  
pp. 87-93 ◽  
Author(s):  
Matthew K. Gould ◽  
Xuan Lan Vu ◽  
Thomas Seebeck ◽  
Harry P. de Koning
Keyword(s):  
Propidium Iodide ◽  
Drug Action ◽  
Alamar Blue Assay ◽  
Alamar Blue ◽  
Monitoring Drug

scholarly journals Synthesis andIn VitroEvaluation of New Thiosemicarbazone Derivatives as Potential Antimicrobial Agents

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Zafer Asım Kaplancıklı ◽  
Mehlika Dilek Altıntop ◽  
Belgin Sever ◽  
Zerrin Cantürk ◽  
Ahmet Özdemir
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Aromatic Aldehydes ◽  
Antitubercular Activity ◽  
Alamar Blue Assay ◽  
Alamar Blue ◽  
Inhibitory Effects ◽  
Microdilution Method ◽  
Broth Microdilution Method

In an effort to develop potent antimicrobial agents, new thiosemicarbazone derivatives were synthesizedviathe reaction of 4-[4-(trifluoromethyl)phenyl]thiosemicarbazide with aromatic aldehydes. The compounds were evaluated for their inhibitory effects on pathogenic bacteria and yeasts using the CLSI broth microdilution method. Microplate Alamar Blue Assay was also carried out to determine the antimycobacterial activities of the compounds againstMycobacterium tuberculosisH37Rv. Among these derivatives, compounds5and11were more effective againstEnterococcus faecalis(ATCC 29212) than chloramphenicol, whereas compounds1,2, and12and chloramphenicol showed the same level of antibacterial activity againstE. faecalis. Moreover, compound2and chloramphenicol exhibited the same level of antibacterial activity againstStaphylococcus aureus. On the other hand, the most potent anticandidal derivatives were found as compounds2and5. These derivatives and ketoconazole exhibited the same level of antifungal activity againstCandida glabrata. According to the Microplate Alamar Blue Assay, the tested compounds showed weak to moderate antitubercular activity.

scholarly journals Rapid, Low-Technology MIC Determination with Clinical Mycobacterium tuberculosis Isolates by Using the Microplate Alamar Blue Assay

1998 ◽  
Vol 36 (2) ◽  
pp. 362-366 ◽  
Author(s):  
Scott G. Franzblau ◽  
Richard S. Witzig ◽  
James C. McLaughlin ◽  
Patricia Torres ◽  
Guillermo Madico ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Low Cost ◽  
Multidrug Resistant ◽  
Appropriate Technology ◽  
Alamar Blue Assay ◽  
Alamar Blue ◽  
Solid Media ◽  
Resistant Strains ◽  
Stable Reagent ◽  
Initial Results

A colorimetric, microplate-based Alamar Blue assay (MABA) method was used to determine the MICs of isoniazid (INH), rifampin, streptomycin (SM), and ethambutol (EMB) for 34 PeruvianMycobacterium tuberculosis isolates (including both pansensitive and multidrug-resistant strains) and the H37Rv strain by using bacterial suspensions prepared directly from solid media. Results for all isolates were available within 8 days. Discordant results were observed on initial tests for 3 of 16 INH-susceptible isolates, 5 of 31 EMB-susceptible isolates, and 2 of 4 SM-resistant isolates (by the BACTEC 460 system). The overall agreements between the MICs obtained by MABA and the results obtained with the BACTEC 460 system were 87.9% for initial results and 93.6% after retesting 12 of 17 samples with discrepant results. Interpretation of MABA endpoints improved with technical experience. The MABA is a simple, rapid, low-cost, appropriate technology which does not require expensive instrumentation and which makes use of a nontoxic, temperature-stable reagent.

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