Methods for Investigating Cell Division Mechanisms in C. elegans

2022 ◽  
pp. 19-35
Author(s):  
Ian D. Wolff ◽  
Nikita S. Divekar ◽  
Sarah M. Wignall
Keyword(s):  
Cell Division ◽  
C Elegans

scholarly journals Cortical localization of the G  protein GPA-16 requires RIC-8function during C. elegans asymmetric cell division

Development ◽  
2005 ◽  
Vol 132 (20) ◽  
pp. 4449-4459 ◽  
Author(s):  
K. Afshar ◽  
F. S. Willard ◽  
K. Colombo ◽  
D. P. Siderovski ◽  
P. Gonczy
Keyword(s):  
Cell Division ◽  
G Protein ◽  
Asymmetric Cell Division ◽  
C Elegans ◽  
Cortical Localization

scholarly journals Extracellular control of PAR protein localization during asymmetric cell division in the C. elegans embryo

Development ◽  
2010 ◽  
Vol 137 (19) ◽  
pp. 3337-3345 ◽  
Author(s):  
Y. Arata ◽  
J.-Y. Lee ◽  
B. Goldstein ◽  
H. Sawa
Keyword(s):  
Cell Division ◽  
Asymmetric Cell Division ◽  
Protein Localization ◽  
C Elegans

scholarly journals Regulation of anterior cell-specific mec-3 expression during asymmetric cell division in C. elegans

1992 ◽  
Vol 194 (4) ◽  
pp. 289-302 ◽  
Author(s):  
Jeffrey C. Way ◽  
Jin-Quan Run ◽  
Alice Y. Wang
Keyword(s):  
Cell Division ◽  
Asymmetric Cell Division ◽  
C Elegans

scholarly journals C. elegans Nucleostemin Is Required for Larval Growth and Germline Stem Cell Division

PLoS Genetics ◽  
2008 ◽  
Vol 4 (8) ◽  
pp. e1000181 ◽  
Author(s):  
Michelle M. Kudron ◽  
Valerie Reinke
Keyword(s):  
Stem Cell ◽  
Cell Division ◽  
Larval Growth ◽  
Germline Stem Cell ◽  
C Elegans ◽  
Stem Cell Division

scholarly journals Suspended Animation Extends Survival Limits of Caenorhabditis elegans and Saccharomyces cerevisiae at Low Temperature

2010 ◽  
Vol 21 (13) ◽  
pp. 2161-2171 ◽  
Author(s):  
Kin Chan ◽  
Jesse P. Goldmark ◽  
Mark B. Roth
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Cell Division ◽  
Caenorhabditis Elegans ◽  
Cell Division Cycle ◽  
Wild Type ◽  
Suspended Animation ◽  
C Elegans ◽  
High Viability ◽  
Cold Sensitive

The orderly progression through the cell division cycle is of paramount importance to all organisms, as improper progression through the cycle could result in defects with grave consequences. Previously, our lab has shown that model eukaryotes such as Saccharomyces cerevisiae, Caenorhabditis elegans, and Danio rerio all retain high viability after prolonged arrest in a state of anoxia-induced suspended animation, implying that in such a state, progression through the cell division cycle is reversibly arrested in an orderly manner. Here, we show that S. cerevisiae (both wild-type and several cold-sensitive strains) and C. elegans embryos exhibit a dramatic decrease in viability that is associated with dysregulation of the cell cycle when exposed to low temperatures. Further, we find that when the yeast or worms are first transitioned into a state of anoxia-induced suspended animation before cold exposure, the associated cold-induced viability defects are largely abrogated. We present evidence that by imposing an anoxia-induced reversible arrest of the cell cycle, the cells are prevented from engaging in aberrant cell cycle events in the cold, thus allowing the organisms to avoid the lethality that would have occurred in a cold, oxygenated environment.

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