Chromatin Immunoprecipitation Protocol for Circadian Clock Proteins

2021 ◽  
pp. 135-150
Author(s):  
Sally Adams ◽  
Isabelle A. Carré
Keyword(s):  
Circadian Clock ◽  
Chromatin Immunoprecipitation ◽  
Clock Proteins ◽  
Circadian Clock Proteins

scholarly journals Circadian Clock Proteins in Prokaryotes: Hidden Rhythms?

2010 ◽  
Vol 1 ◽  
Author(s):  
Maria Loza-Correa ◽  
Laura Gomez-Valero ◽  
Carmen Buchrieser
Keyword(s):  
Circadian Clock ◽  
Clock Proteins ◽  
Circadian Clock Proteins

scholarly journals Attenuated SIRT1 Activity Leads to PER2 Cytoplasmic Localization and Dampens the Amplitude of Bmal1 Promoter-Driven Circadian Oscillation

2021 ◽  
Vol 15 ◽  
Author(s):  
Atsushige Ashimori ◽  
Yasukazu Nakahata ◽  
Toshiya Sato ◽  
Yuichiro Fukamizu ◽  
Takaaki Matsui ◽  
...  
Keyword(s):  
Subcellular Localization ◽  
Circadian Clock ◽  
Posttranslational Modifications ◽  
Cultured Cells ◽  
Circadian Oscillation ◽  
Clock Proteins ◽  
Circadian Clock Proteins ◽  
Circadian Physiology ◽  
And Behavior ◽  
Robust Systems

The circadian clock possesses robust systems to maintain the rhythm approximately 24 h, from cellular to organismal levels, whereas aging is known to be one of the risk factors linked to the alternation of circadian physiology and behavior. The amount of many metabolites in the cells/body is altered with the aging process, and the most prominent metabolite among them is the oxidized form of nicotinamide adenine dinucleotide (NAD+), which is associated with posttranslational modifications of acetylation and poly-ADP-ribosylation status of circadian clock proteins and decreases with aging. However, how low NAD+ condition in cells, which mimics aged or pathophysiological conditions, affects the circadian clock is largely unknown. Here, we show that low NAD+ in cultured cells promotes PER2 to be retained in the cytoplasm through the NAD+/SIRT1 axis, which leads to the attenuated amplitude of Bmal1 promoter-driven luciferase oscillation. We found that, among the core clock proteins, PER2 is mainly affected in its subcellular localization by NAD+ amount, and a higher cytoplasmic PER2 localization was observed under low NAD+ condition. We further found that NAD+-dependent deacetylase SIRT1 is the regulator of PER2 subcellular localization. Thus, we anticipate that the altered PER2 subcellular localization by low NAD+ is one of the complex changes that occurs in the aged circadian clock.

scholarly journals 2PT229 The regulation of cyanobacterial circadian clock proteins KaiB-KaiC interaction by ATP(The 50th Annual Meeting of the Biophysical Society of Japan)

2012 ◽  
Vol 52 (supplement) ◽  
pp. S143
Author(s):  
Risa Mutoh ◽  
Atsuhito Nishimura ◽  
So Yasui ◽  
Kiyoshi Onai ◽  
Masahiro Ishiura
Keyword(s):  
Circadian Clock ◽  
Annual Meeting ◽  
Clock Proteins ◽  
Biophysical Society ◽  
Circadian Clock Proteins

scholarly journals Relationship between HIF-1 and Circadian Clock Proteins in Obstructive Sleep Apnea Patients—Preliminary Study

2020 ◽  
Vol 9 (5) ◽  
pp. 1599 ◽  
Author(s):  
Agata Gabryelska ◽  
Marcin Sochal ◽  
Szymon Turkiewicz ◽  
Piotr Białasiewicz
Keyword(s):  
Obstructive Sleep Apnea ◽  
Sleep Apnea ◽  
Circadian Clock ◽  
Protein Level ◽  
Hypoxia Inducible Factor ◽  
Apnea Hypopnea Index ◽  
Clock Proteins ◽  
Obstructive Sleep ◽  
Healthy Control ◽  
Circadian Clock Proteins

Obstructive sleep apnea (OSA) is characterized by intermittent hypoxia and associated with the disruption of circadian rhythm. The study aimed to assess the relationship between hypoxia-inducible factor (HIF) subunits, circadian clock proteins, and polysomnography (PSG) variables, in healthy individuals and severe OSA patients. The study included 20 individuals, who underwent PSG and were divided into severe OSA group (n = 10; AHI ≥ 30) and healthy control (n = 10; AHI < 5) based on apnea-hypopnea index (AHI). All participants had their peripheral blood collected in the evening before and the morning after the PSG. HIF-1α, HIF-1β, BMAL1, CLOCK, CRY1, and PER1 protein concertation measurements were performed using ELISA. In a multivariate general linear model with the concentration of all circadian clock proteins as dependent variables, evening HIF-1α protein level was the only significant covariant (p = 0.025). Corrected models were significant for morning and evening PER1 (p = 0.008 and p = 0.006, respectively), evening (p = 0.043), and evening BMAL protein level (p = 0.046). In corrected models, evening HIF-1α protein level had an influence only on the evening PER1 protein level. Results suggest that OSA patients are at risk for developing circadian clock disruption. This process might be mediated by subunit α of HIF-1, as its increased protein level is associated with overexpression of circadian clock proteins.

scholarly journals SnapShot: Circadian Clock Proteins

Cell ◽  
2008 ◽  
Vol 135 (2) ◽  
pp. 368-368.e1 ◽  
Author(s):  
Elizabeth E. Hamilton ◽  
Steve A. Kay
Keyword(s):  
Circadian Clock ◽  
Clock Proteins ◽  
Circadian Clock Proteins

Structural and biochemical characterization of Drosophila and mammalian circadian clock proteins

2007 ◽  
Vol 2007 (Fall) ◽  
Author(s):  
Eva Wolf ◽  
Sven Hennig ◽  
Alex Berndt ◽  
Helena Breitkreuz ◽  
Özkan Yildiz ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Biochemical Characterization ◽  
Clock Proteins ◽  
Circadian Clock Proteins

Modulation of glucocorticoid receptor induction properties by core circadian clock proteins

2014 ◽  
Vol 383 (1-2) ◽  
pp. 170-180 ◽  
Author(s):  
Dong-Hee Han ◽  
Yeon-Ju Lee ◽  
Kyungjin Kim ◽  
Chang-Ju Kim ◽  
Sehyung Cho
Keyword(s):  
Glucocorticoid Receptor ◽  
Circadian Clock ◽  
Clock Proteins ◽  
Circadian Clock Proteins

scholarly journals Circadian clock proteins regulate neuronal redox homeostasis and neurodegeneration

2013 ◽  
Vol 123 (12) ◽  
pp. 5389-5400 ◽  
Author(s):  
Erik S. Musiek ◽  
Miranda M. Lim ◽  
Guangrui Yang ◽  
Adam Q. Bauer ◽  
Laura Qi ◽  
...  
Keyword(s):  
Circadian Clock ◽  
Redox Homeostasis ◽  
Clock Proteins ◽  
Circadian Clock Proteins
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